Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways

Abstract. We present the results of a native fluorescence spectroscopy study of blood plasma of rats with experimental diabetes. It was shown that the fluorescence emission band shape at 320 nm excitation is the most indicative of hyperglycemia in the blood plasma samples. We provide the interpretation of this fact based on the changes in reduced nicotinamide adenine dinucleotide phosphate concentration due to glucose-related metabolic pathways and protein fluorescent cross-linking formation following nonenzymatic glycation.

[1]  I. N. Smirnova,et al.  Application of time-domain THz spectroscopy for studying blood plasma of rats with experimental diabetes , 2014 .

[2]  Vadivel Masilamani,et al.  Fluorescence spectra of blood and urine for cervical cancer detection , 2012, Journal of biomedical optics.

[3]  H. Nielsen,et al.  Fluorescence spectroscopy as a potential metabonomic tool for early detection of colorectal cancer , 2012, Metabolomics.

[4]  N. V. Kuznetsova,et al.  Adrenocortical system activity in alloxan-resistant and alloxan-susceptible Wistar rats , 2012 .

[5]  R. Dasari,et al.  Raman Spectroscopy Provides a Powerful Diagnostic Tool for Accurate Determination of Albumin Glycation , 2012, PloS one.

[6]  N. V. Kuznetsova,et al.  Activity of the adrenocortical system in rats with experimental diabetes , 2011 .

[7]  M. Leone,et al.  Thermal aggregation of glycated bovine serum albumin. , 2010, Biochimica et biophysica acta.

[8]  Dorte Vistisen,et al.  Global healthcare expenditure on diabetes for 2010 and 2030. , 2010, Diabetes research and clinical practice.

[9]  W. Ying.  NAD+/NADH and NADP+/NADPH in cellular functions and cell death: regulation and biological consequences. , 2008, Antioxidants & redox signaling.

[10]  V. Masilamani,et al.  Cancer diagnosis by autofluorescence of blood components , 2004 .

[11]  S. Madhuri,et al.  Native Fluorescence Spectroscopy of Blood Plasma in the Characterization of Oral Malignancy ¶ , 2003, Photochemistry and photobiology.

[12]  J. Baynes,et al.  Maillard reaction products in tissue proteins: New products and new perspectives , 2003, Amino Acids.

[13]  T A Woolsey,et al.  NADH: sensor of blood flow need in brain, muscle, and other tissues , 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[14]  P. Callis,et al.  Mechanisms of tryptophan fluorescence shifts in proteins. , 2001, Biophysical journal.

[15]  H. Parving,et al.  Amadori albumin in type 1 diabetic patients: correlation with markers of endothelial function, association with diabetic nephropathy, and localization in retinal capillaries. , 1999, Diabetes.

[16]  S. Menini,et al.  Mutual interaction between glycation and oxidation during non-enzymatic protein modification. , 1997, Biochimica et biophysica acta.

[17]  D. Koteeswaran,et al.  Ultraviolet fluorescence spectroscopy of blood plasma in the discrimination of cancer from normal , 1997, Photonics West - Biomedical Optics.

[18]  J. Nyengaard,et al.  Hyperglycemic Pseudohypoxia and Diabetic Complications , 1993, Diabetes.

[19]  J. Baynes,et al.  Accumulation of Maillard Reaction Products in Skin Collagen in Diabetes and Aging a , 1992, Annals of the New York Academy of Sciences.

[20]  R R Alfano,et al.  Light sheds light on cancer--distinguishing malignant tumors from benign tissues and tumors. , 1991, Bulletin of the New York Academy of Medicine.

[21]  V. Monnier,et al.  Structure elucidation of a senescence cross-link from human extracellular matrix. Implication of pentoses in the aging process. , 1989, The Journal of biological chemistry.

[22]  A. Oronsky,et al.  Nonenzymatic glycation of bovine serum albumin by fructose (fructation). Comparison with the Maillard reaction initiated by glucose. , 1989, The Journal of biological chemistry.

[23]  O. Wolfbeis,et al.  Fluorescence topography in biology. III: Characteristic deviations of tryptophan fluorescence in sera of patients with gynecological tumors. , 1986, Clinical chemistry.

[24]  C. Elmets,et al.  Relation between complications of type I diabetes mellitus and collagen-linked fluorescence. , 1986, The New England journal of medicine.

[25]  A. Cerami,et al.  Nonenzymatic glycosylation and the pathogenesis of diabetic complications. , 1984, Annals of internal medicine.

[26]  H. Kato.  [Maillard reaction in vivo]. , 1984, Seikagaku. The Journal of Japanese Biochemical Society.

[27]  J. Casciari,et al.  The assessment of the energy metabolism in patients with chronic fatigue syndrome by serum fluorescence emission. , 2012, Alternative therapies in health and medicine.

[28]  H. Riordan,et al.  Detection of energy metabolism level in cancer patients by fluorescence emission from serum , 2003 .

[29]  David J S Birch,et al.  Non-invasive glucose monitoring by NAD(P)H autofluorescence spectroscopy in fibroblasts and adipocytes: a model for skin glucose sensing. , 2003, Diabetes technology & therapeutics.

[30]  T Zima,et al.  Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. , 2002, Physiological research.

[31]  D. Harris,et al.  Endogenous porphyrin fluorescence in tumors , 1987, Lasers in surgery and medicine.

[32]  O. Wolfbeis,et al.  Mapping of the total fluorescence of human blood serum as a new method for its characterization , 1985 .

[33]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .