Insect larval expression process is optimized by generating fusions with green fluorescent protein.
暂无分享,去创建一个
W. Bentley | G. Rao | H. Cha | V. Vakharia | N. G. Dalal | M. Pham | Hyung Joon Cha | Govind Rao | William E. Bentley
[1] W. Bentley,et al. Expression and purification of human interleukin-2 simplified as a fusion with green fluorescent protein in suspended Sf-9 insect cells. , 1999, Journal of biotechnology.
[2] W. Bentley,et al. Expression of green fluorescent protein in insect larvae and its application for heterologous protein production. , 1997, Biotechnology and bioengineering.
[3] C. Albano,et al. On-line green fluorescent protein sensor with LED excitation. , 1997, Biotechnology and bioengineering.
[4] W. Stemmer,et al. Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling , 1996, Nature Biotechnology.
[5] K. Gopinathan,et al. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. , 1995, BioTechniques.
[6] G. Schaffner,et al. Cancer vaccines: the interleukin 2 dosage effect. , 1995, Proceedings of the National Academy of Sciences of the United States of America.
[7] W. Bentley,et al. Purification of a recombinant protein produced in a baculovirus expression system by immobilized metal affinity chromatography , 1994, Biotechnology and bioengineering.
[8] M. Chalfie,et al. Green fluorescent protein as a marker for gene expression. , 1994, Science.
[9] B. Kennedy,et al. Human group II phospholipase A2 expressed in Trichoplusia ni larvae--isolation and kinetic properties of the enzyme. , 1993, Protein expression and purification.
[10] W. Dawson,et al. Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector. , 1991, Proceedings of the National Academy of Sciences of the United States of America.
[11] M. Goosen. Insect cell cultivation techniques for the production of high‐valued products , 1991 .
[12] Y. Okada,et al. Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector , 1990, FEBS letters.
[13] R. K. Evans,et al. Efficient, low-cost protein factories: expression of human adenosine deaminase in baculovirus-infected insect larvae. , 1990, Proceedings of the National Academy of Sciences of the United States of America.
[14] H. Klenk,et al. Synthesis of biologically active influenza virus hemagglutinin in insect larvae , 1989, Journal of virology.
[15] C. Rice,et al. Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. , 1989, Science.
[16] I. R. Cameron,et al. Insect cell culture technology in baculovirus expression systems , 1989 .
[17] L. Tamarkin,et al. Interleukin-2 differentially affects the proliferation of a hormone-dependent and a hormone-independent human breast cancer cell line in vitro and in vivo. , 1988, Anticancer research.
[18] P. Earl,et al. Characterization of human immunodeficiency virus gag/pol gene products expressed by recombinant vaccinia viruses. , 1988, Virology.
[19] H. Müller,et al. Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. , 1988, The Journal of general virology.
[20] J. Vlak,et al. Immunogold Detection of Polyhedrin, p10 and Virion Antigens in Autographa californica Nuclear Polyhedrosis Virus-infected Spodoptera frugiperda Cells , 1987 .
[21] M. Furusawa,et al. Hyperproduction of polyhedrin-IGF II fusion protein in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrosis virus. , 1987, The Journal of general virology.
[22] M. Furusawa,et al. Production of human α-interferon in silkworm using a baculovirus vector , 1985, Nature.
[23] M. Doyle,et al. Biological activity of recombinant human interleukin-2 produced in Escherichia coli. , 1984, Science.
[24] M. Summers,et al. Production of human beta interferon in insect cells infected with a baculovirus expression vector , 1983, Molecular and cellular biology.
[25] M. Summers,et al. Molecular Engineering of the Autographa californica Nuclear Polyhedrosis Virus Genome: Deletion Mutations Within the Polyhedrin Gene , 1983, Journal of virology.
[26] R. Matthews. Classification and Nomenclature of Viruses: 3rd Report of the International Committe on Taxonomy of Viruses , 1980 .
[27] K. Harrap. The structure of nuclear polyhedrosis viruses. I. The inclusion body. , 1972, Virology.
[28] U. K. Laemmli,et al. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.
[29] W. Bentley,et al. Purification of Human Interleukin‐2 Fusion Protein Produced in Insect Larvae Is Facilitated by Fusion with Green Fluorescent Protein and Metal Affinity Ligand , 1999, Biotechnology progress.
[30] L. King,et al. The Baculovirus Expression System , 1992 .
[31] D. O'reilly,et al. Baculovirus expression vectors: a laboratory manual. , 1992 .
[32] M. Summers,et al. Trends in the Development of Baculovirus Expression Vectors , 1988, Bio/Technology.
[33] L. Miller. Baculoviruses as gene expression vectors. , 1988, Annual review of microbiology.
[34] K. Arai,et al. Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3. , 1987, Gene.
[35] M. Perkus,et al. Vaccinia virus as an expression vector. , 1987, Methods in enzymology.