Evolutionary optimisation of enzymes.

Aside from the demonstration that individual molecular traits of enzymes can be evolutionarily optimised, the discovery that several traits can be simultaneously optimised is a major advance. The first observations of the effects of evolutionary optimisation at the structural level, through X-ray crystallography, reinforce the view that enzymes are best optimised by evolution and not by design.

[1]  W. Stemmer.  Rapid evolution of a protein in vitro by DNA shuffling , 1994, Nature.

[2]  J. Hoseki,et al.  Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. , 1999, Journal of biochemistry.

[3]  D. Herschlag,et al.  Catalytic promiscuity and the evolution of new enzymatic activities. , 1999, Chemistry & biology.

[4]  F. Arnold,et al.  A structural view of evolutionary divergence. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[5]  L. Hansson,et al.  Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling. , 1999, Journal of molecular biology.

[6]  F. Arnold,et al.  Functional Expression of Horseradish Peroxidase in E.coli by Directed Evolution , 1999, Biotechnology progress.

[7]  P. Schultz,et al.  Progress toward the evolution of an organism with an expanded genetic code. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[8]  S. Harayama,et al.  Novel family shuffling methods for the in vitro evolution of enzymes. , 1999, Gene.

[9]  U. Bornscheuer,et al.  Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay , 1999, Biological chemistry.

[10]  D. M. Brown,et al.  An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. , 1996, Journal of molecular biology.

[11]  R. Roberts,et al.  In vitro selection of nucleic acids and proteins: What are we learning? , 1999, Current opinion in structural biology.

[12]  F. Arnold,et al.  Directed evolution of biocatalysts. , 1999, Current opinion in chemical biology.

[13]  J. Minshull,et al.  Protein evolution by molecular breeding. , 1999, Current opinion in chemical biology.

[14]  R. Ward,et al.  The Amino Acid Sequence of Ribitol Dehydrogenase-F, a Mutant Enzyme with Improved Xylitol Dehydrogenase Activity , 1999, Journal of protein chemistry.

[15]  S. Gould.  The Panda's Thumb , 1980 .

[16]  Manfred T Reetz,et al.  A Method for High-Throughput Screening of Enantioselective Catalysts. , 1999, Angewandte Chemie.

[17]  F. Cross,et al.  Directed evolution to bypass cyclin requirements for the Cdc28p cyclin-dependent kinase. , 1999, Molecular cell.

[18]  F. Arnold,et al.  Directed evolution converts subtilisin E into a functional equivalent of thermitase. , 1999, Protein engineering.

[19]  W. Stemmer,et al.  DNA shuffling of a family of genes from diverse species accelerates directed evolution , 1998, Nature.

[20]  G. Winter,et al.  A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage. , 1999, Journal of molecular biology.

[21]  Gerd Folkers,et al.  Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling , 1999, Nature Biotechnology.

[22]  Palle Schneider,et al.  Directed evolution of a fungal peroxidase , 1999, Nature Biotechnology.

[23]  B. Hall.  Experimental evolution of Ebg enzyme provides clues about the evolution of catalysis and to evolutionary potential. , 1999, FEMS microbiology letters.

[24]  U. Bornscheuer,et al.  Directed evolution of an esterase: screening of enzyme libraries based on pH-indicators and a growth assay. , 1999, Bioorganic & medicinal chemistry.

[25]  M. Zaccolo,et al.  The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. , 1999, Journal of molecular biology.

[26]  Fengzhu Sun.  Modeling DNA Shuffling , 1999, J. Comput. Biol..

[27]  Andrew D. Ellington,et al.  Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase , 1999, Nature Biotechnology.

[28]  F. Arnold,et al.  A high-throughput digital imaging screen for the discovery and directed evolution of oxygenases. , 1999, Chemistry & biology.

[29]  Jon E. Ness,et al.  DNA shuffling of subgenomic sequences of subtilisin , 1999, Nature Biotechnology.

[30]  A. Okamoto,et al.  Redesigning the Substrate Specificity of an Enzyme by Cumulative Effects of the Mutations of Non-active Site Residues* , 1999, The Journal of Biological Chemistry.

[31]  W. K. Wilson,et al.  Directed Evolution To Investigate Steric Control of Enzymatic Oxidosqualene Cyclization. An Isoleucine-to-Valine Mutation in Cycloartenol Synthase Allows Lanosterol and Parkeol Biosynthesis , 1999 .

[32]  Alan R. Fersht,et al.  Directed evolution of new catalytic activity using the α/β-barrel scaffold , 2000, Nature.

[33]  F. Arnold,et al.  Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation , 1999, Nature.

[34]  M. Reetz,et al.  Superior Biocatalysts by Directed Evolution , 1999 .