Analysis of lndole-3-Acetic Acid Metabolites from Dalbergia dolichopetala by High Performance Liquid Chromatography-Mass Spectrometry'

A mixture of [2-14C,] and [P3C6Jindole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain ["4Cl,"3C6]indole-3-acetylas-partic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction 11 revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic

[1]  Jerry D. Cohen,et al.  Indole-3-acetic acid and indole-3-acetylaspartic acid isolated from seeds of Heracleum laciniatum Horn , 1991, Plant Growth Regulation.

[2]  G. Sandberg,et al.  The biosynthesis and conjugation of indole-3-acetic acid in germinating seed and seedlings ofDalbergia dolichopetala , 1988, Planta.

[3]  L. Eliasson,et al.  Levels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttings , 1991 .

[4]  T. Jenny,et al.  IAA-induced adventitious root formation in greenwood cuttings of Populus tremula and formation of 2-indolone-3-acetylaspartic acid, a new metabolite of exogeneously applied indole-3-acetic acid , 1989 .

[5]  G. Sandberg,et al.  Endogenous Hormones, Germination and Early Seedling Growth of Dalbergia dolicbopetala , 1988 .

[6]  G. Sandberg,et al.  Detection of abscisic acid, indole-3-acetic acid and indole-3-ethanol in seeds of Dalbergia dolichopetala , 1987 .

[7]  R. Bandurski,et al.  Occurrence and metabolism of 7-hydroxy-2-indolinone-3-acetic acid in Zea mays. , 1987, Phytochemistry.

[8]  S. Tsurumi,et al.  Dioxindole-3-Acetic Acid Conjugates Formation from Indole-3-Acetylaspartic Acid in Vicia Seedlings , 1986 .

[9]  G. Sandberg,et al.  Effects of sodium diethyldithiocarbamate, solvent, temperature and plant extracts on the stability of indoles , 1986 .

[10]  R. Bandurski,et al.  Transport and metabolism of indole-3-acetyl-myo-inositol-galactoside in seedlings of Zea mays. , 1986, Plant physiology.

[11]  H. Nonhebel,et al.  Oxidation of indole-3-acetic acid and oxindole-3-acetic acid to 2,3-dihydro-7-hydroxy-2-oxo-1H indole-3-acetic acid-7'-O-beta-D-glucopyranoside in Zea mays seedlings. , 1984, Plant physiology.

[12]  J. Cohen,et al.  Indole-3-acetic Acid (IAA) and IAA Conjugates Applied to Bean Stem Sections: IAA Content and the Growth Response. , 1983, Plant physiology.

[13]  J. Cohen.  Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L. , 1982, Plant physiology.

[14]  R. Pharis,et al.  Use of insoluble polyvinylpyrrolidone for purification of plant extracts and chromatography of plant hormones , 1972 .