Assessing Copy Number of MON 810 Integrations in Commercial Seed Maize Varieties by 5′ Event-Specific Real-Time PCR Validated Method Coupled to $$2^{ - \Delta \Delta CT} $$ Analysis
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G. Bellocchi | M. Querci | A. Milcamps | G. Eede | M. Aguilera | S. Pastor
[1] M. Querci,et al. A Qualitative Approach for the Assessment of the Genetic Stability of the MON 810 Trait in Commercial Seed Maize Varieties , 2008 .
[2] K. Gruden,et al. Method Validation and Quality Management in the Flexible Scope of Accreditation: An Example of Laboratories Testing for Genetically Modified Organisms , 2008 .
[3] Roberto Confalonieri,et al. Expanding Horizons in the Validation of GMO Analytical Methods: Fuzzy-based Expert Systems , 2008 .
[4] K. Gruden,et al. Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms , 2008, BMC biotechnology.
[5] V. Laval,et al. A strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa). , 2007, Journal of agricultural and food chemistry.
[6] José Luis La Paz,et al. Comparison of real-time PCR detection chemistries and cycling modes using Mon810 event-specific assays as model. , 2007, Journal of agricultural and food chemistry.
[7] P. Corbisier,et al. Toward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON 810 corn model. , 2007, Journal of agricultural and food chemistry.
[8] Sabrina Gioria,et al. Toward metrological traceability for DNA fragment ratios in GM quantification. 1. Effect of DNA extraction methods on the quantitative determination of Bt176 corn by real-time PCR. , 2007, Journal of agricultural and food chemistry.
[9] P. Corbisier,et al. Toward metrological traceability for DNA fragment ratios in GM quantification. 2. Systematic study of parameters influencing the quantitative determination of MON 810 corn by real-time PCR. , 2007, Journal of agricultural and food chemistry.
[10] L. Martinelli,et al. Development of a real-time PCR method based on duplo target plasmids for determining an unexpected genetically modified soybean intermix with feed components. , 2007, Journal of agricultural and food chemistry.
[11] Litao Yang,et al. Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule. , 2007, Journal of agricultural and food chemistry.
[12] A. Depicker,et al. Sequence stability of the T-DNA – plant junctions in tissue culture in Arabidopsis transgenic lines , 2006, Plant Cell Reports.
[13] R. Aubin,et al. Accurate Determination of Zygosity in Transgenic Rice by Real-time PCR Does Not Require Standard Curves or Efficiency Correction , 2006, Transgenic Research.
[14] Marta Hernández,et al. Current Methodology for Detection, Identification and Quantification of Genetically Modified Organisms , 2005 .
[15] I. Baldwin,et al. Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR , 2004, BMC biotechnology.
[16] Marta Hernández,et al. Development and comparison of four real-time polymerase chain reaction systems for specific detection and quantification of Zea mays L. , 2004, Journal of agricultural and food chemistry.
[17] S. Prat,et al. A Specific Real-Time Quantitative PCR Detection System for Event MON810 in Maize YieldGard® Based on the 3′-Transgene Integration Sequence , 2003, Transgenic Research.
[18] Paolo Provero,et al. Estimating the number of integrations in transformed plants by quantitative real-time PCR , 2002, BMC biotechnology.
[19] Knut Rudi,et al. 5'-Nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize , 2002 .
[20] F. Speleman,et al. Quantification of MYCN, DDX1, and NAG Gene Copy Number in Neuroblastoma Using a Real-Time Quantitative PCR Assay , 2002, Modern Pathology.
[21] Thomas D. Schmittgen,et al. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.
[22] D J Ingham,et al. Quantitative real-time PCR assay for determining transgene copy number in transformed plants. , 2001, BioTechniques.
[23] R. Twyman,et al. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns , 2000, Transgenic Research.
[24] G. Martin,et al. Chromosome 2-specific DNA clones from flow-sorted chromosomes of tomato , 1994, Molecular and General Genetics MGG.