Taxonomic status of the Japanese triploid forms of Fasciola: comparison of mitochondrial ND1 and COI sequences with F. hepatica and F. gigantica.

Because it is difficult to identify morphologically the Japanese forms of Fasciola, additional taxonomic criteria are required. In order to clarify the genetic relationships between Fasciola hepatica, Fasciola gigantica, and the Japanese triploid forms of Fasciola, we compared nucleotide sequences of mitochondrial NADH dehydrogenase subunit 1 (ND1) and cytochrome c oxidase subunit I (COI) genes. Intraspecific variation in the ND1 and COI sequences was low, except for 1 specimen of F. gigantica in the ND1 sequence. The ND1 and COI sequences of Japanese triploid forms of Fasciola were nearly identical to those found in F. gigantica but were different from those of F. hepatica. Thus, the Japanese triploid forms of Fasciola are thought to be categorized as F. gigantica.

[1]  D. McManus,et al.  Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. , 1996, International journal for parasitology.

[2]  堤 可厚,et al.  Characterization of Genetic Divergence Among Species within the Genus Fasciola by PCR-SSCP , 1995 .

[3]  D. McManus,et al.  NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. , 1993, International journal for parasitology.

[4]  S. Barker,et al.  Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea). , 1993, International journal for parasitology.

[5]  D. McManus,et al.  Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. , 1992, Molecular and biochemical parasitology.

[6]  C. Combes,et al.  Isolation and polymorphism in mitochondrial DNA from Schistosoma mansoni. , 1991, Molecular and biochemical parasitology.

[7]  F. Collins,et al.  Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. , 1991, Nucleic acids research.

[8]  D. McManus,et al.  Restriction enzyme mapping of ribosomal DNA can distinguish between fasciolid (liver fluke) species. , 1989, Molecular and biochemical parasitology.

[9]  Y. Sakaguchi Karyotype and gametogenesis of the common liver fluke, Fasciola sp., in Japan , 1980 .

[10]  H. Itagaki,et al.  Morphological study on the Japanese liver fluke, compared with the African specimens. , 1959 .