Protection of mice from infection with Streptococcus pneumoniae by anti-phosphocholine antibody

Anti-phosphocholine (PC) antibody mediated protection against many strains of Streptococcus pneumoniae, and hybridoma anti-PC antibodies protected mice from fatal infections with types 1 and 3 S. pneumoniae. Live types 1, 3, 5, 6A, and 19F S. pneumoniae had similar amounts of surface PC accessible to antibody. Furthermore, mice expressing the X-linked immunodeficiency (xid) of the CBA/N strain were found to be more susceptible to infection with S. pneumoniae of types 3, 6A, and 19F than were immunologically normal mice. The only exception to these results was with the type 5 strain, which was highly virulent for both xid and normal mice. In addition, we were unable to protect mice against infection with the type 5 strain by using anti-PC antibody.

[1]  R. Perlmutter,et al.  Immune response deficiency of BSVS mice. II. Generalized deficiency to thymus‐dependent antigens , 1979, European journal of immunology.

[2]  D. Brundish,et al.  Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. , 1968, The Biochemical journal.

[3]  S. Hudak,et al.  Anti-phosphocholine hybridoma antibodies. I. Direct evidence for three distinct families of antibodies in the murine response , 1981, The Journal of experimental medicine.

[4]  D. Briles,et al.  Mouse IgG3 antibodies are highly protective against infection with Streptococcus pneumoniae , 1981, Nature.

[5]  W. Wood,et al.  THE INHIBITION OF SURFACE PHAGOCYTOSIS BY THE CAPSULAR "SLIME LAYER" OF PNEUMOCOCCUS TYPE III , 1949, The Journal of experimental medicine.

[6]  T. Eisenstein,et al.  Biological properties of an immunogenic pneumococcal subcellular preparation , 1976, Infection and immunity.

[7]  F. Greenwood,et al.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. , 1963, The Biochemical journal.

[8]  W. Tillett.  ACTIVE AND PASSIVE IMMUNITY TO PNEUMOCOCCUS INFECTION INDUCED IN RABBITS BY IMMUNIZATION WITH R PNEUMOCOCCI , 1928, The Journal of experimental medicine.

[9]  M. Heidelberger,et al.  PREVENTION OF PNEUMOCOCCAL PNEUMONIA BY IMMUNIZATION WITH SPECIFIC CAPSULAR POLYSACCHARIDES , 1945, The Journal of experimental medicine.

[10]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[11]  D. Briles,et al.  Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 streptococcus pneumoniae , 1981, The Journal of experimental medicine.

[12]  E. Gotschlich.  A simplification of the radioactive antigen-binding test by a double label technique. , 1971, Journal of immunology.

[13]  A. Tomasz,et al.  Pneumococcal Forssman antigen. A choline-containing lipoteichoic acid. , 1973, The Journal of biological chemistry.

[14]  T. Eisenstein,et al.  Nature of the cross-protective antigen in subcellular vaccines of Streptococcus pneumoniae , 1981, Infection and immunity.