Electrospun polylactic acid nanofiber membranes as substrates for biosensor assemblies

Abstract Biotin has been successfully incorporated into polylactic acid (PLA) nanofibers through electrospinning to prepare membrane substrates for biosensors based on biotin–streptavidin specific binding. Biotin incorporated PLA nanofiber membranes were characterized with scanning electron microscopy (SEM), electron probe microanalysis (EPMA), and confocal microscopy. Under optimized conditions, small fiber size and uniform morphology were achieved for PLA nanofibers with and without biotin incorporation. Sulfur mapping indicated a non-uniform distribution of biotin on the membranes, presumably due to aggregation of biotin during the electrospinning process. Pre-blocking the membranes effectively eliminated non-specific binding between streptavidin and PLA. Preliminary biosensor assays confirmed that streptavidin immobilized on the membrane surface could capture a biotinylated DNA probe.

[1]  R. Vogel,et al.  A Biosensor-based Immunoassay for Rapid Screening of Deoxynivalenol Contamination in Wheat , 2002 .

[2]  R. W. Tock,et al.  Electrospinning of nanofibers , 2005 .

[3]  Ying Mu,et al.  A novel sandwich immunosensing method for measuring cardiac troponin I in sera. , 2003, Analytical biochemistry.

[4]  Kwangsok Kim,et al.  Structure and process relationship of electrospun bioabsorbable nanofiber membranes , 2002 .

[6]  John Layman,et al.  Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[7]  Xuesi Chen,et al.  Ultrafine fibers electrospun from biodegradable polymers , 2003 .

[8]  Antje J Baeumner,et al.  A generic sandwich-type biosensor with nanomolar detection limits , 2004, Analytical and bioanalytical chemistry.

[9]  Seeram Ramakrishna,et al.  Potential of nanofiber matrix as tissue-engineering scaffolds. , 2005, Tissue engineering.

[10]  Antje J Baeumner,et al.  A universal nucleic acid sequence biosensor with nanomolar detection limits. , 2004, Analytical chemistry.

[11]  R. Pansu,et al.  Polymer beads as nano-sensors , 1999 .

[12]  Antje J Baeumner,et al.  Biosensors for environmental pollutants and food contaminants , 2003, Analytical and bioanalytical chemistry.

[13]  K. Sawicka,et al.  Electrospun biocomposite nanofibers for urea biosensing , 2005 .

[14]  H Morgan,et al.  A surface plasmon resonance immunosensor based on the streptavidin-biotin complex. , 1992, Biosensors & bioelectronics.

[15]  A. Baeumner,et al.  A microfluidic biosensor based on nucleic acid sequence recognition , 2003, Analytical and bioanalytical chemistry.

[16]  S. J. B. Reed,et al.  Electron Microprobe Analysis , 1975 .

[17]  Andreas Greiner,et al.  Nanostructured Fibers via Electrospinning , 2001 .