Change in β-cell mass in Japanese nondiabetic obese individuals.

AIM The aim of this study was to clarify the change in β-cell mass in Japanese obese individuals. METHODS We obtained the pancreas at autopsy from 39 lean and 33 obese Japanese nondiabetic individuals (aged 47 ± 13 vs 47 ± 12 y, P = .83, body mass index 20.4 ± 1.6 vs 28.5 ± 3.9 kg/m(2), P < .01). Pancreatic sections were stained for insulin, and β-cell area (%BCA) was measured as the fraction of the β-cell area to the total pancreas area. β-Cell mass was then calculated as the product of %BCA and estimated pancreas weight. β-Cell replication and apoptosis were assessed by double staining for insulin and Ki67 and insulin and single-stranded DNA, respectively. The frequencies of insulin-positive duct cells and scattered β-cells were assessed as the surrogate markers of β-cell neogenesis. The α-cell area (%ACA) was also measured, and the %ACA to %BCA ratio was determined. RESULTS There was no increase in β-cell mass in obese individuals compared with lean individuals (0.6 ± 0.4 vs 0.7 ± 0.4 g, P = .12). β-Cell replication, β-cell neogenesis, and β-cell apoptosis were not significantly increased in the presence of obesity. There was no significant difference in %ACA to %BCA ratio between obese and lean individuals (0.91 ± 1.09 vs 0.75 ± 0.51, P = .47). CONCLUSION There was no increase in β-cell mass and no detectable change in β-cell turnover in Japanese obese individuals.

[1]  I. Shimomura,et al.  Pancreatic beta cell function in lean and obese Japanese with various degrees of glucose tolerance. , 2013, Endocrine journal.

[2]  R. Rizza,et al.  β-Cell Mass and Turnover in Humans , 2012, Diabetes Care.

[3]  K. Fujihara,et al.  High normal HbA1c levels were associated with impaired insulin secretion without escalating insulin resistance in Japanese individuals: the Toranomon Hospital Health Management Center Study 8 (TOPICS 8) , 2012, Diabetic medicine : a journal of the British Diabetic Association.

[4]  G. Maskarinec,et al.  Pathophysiologic Differences Among Asians, Native Hawaiians, and Other Pacific Islanders and Treatment Implications , 2012, Diabetes Care.

[5]  Hiroshi Ito,et al.  International clinical harmonization of glycated hemoglobin in Japan: From Japan Diabetes Society to National Glycohemoglobin Standardization Program values , 2012, Journal of diabetes investigation.

[6]  M. Yamagishi,et al.  Insulin secretion and insulin sensitivity on the oral glucose tolerance test (OGTT) in middle-aged Japanese. , 2012, Endocrine journal.

[7]  J. Henquin,et al.  Pancreatic alpha cell mass in European subjects with type 2 diabetes , 2011, Diabetologia.

[8]  H. Itoh,et al.  Beta cell dysfunction and its clinical significance in gestational diabetes. , 2010, Endocrine journal.

[9]  S. Bonner-Weir,et al.  β-Cell Growth and Regeneration: Replication Is Only Part of the Story , 2010, Diabetes.

[10]  Kohjiro Ueki,et al.  Report of the Committee on the Classification and Diagnostic Criteria of Diabetes Mellitus , 2010, Journal of diabetes investigation.

[11]  C. Cobelli,et al.  Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy , 2010, Diabetologia.

[12]  C. Cobelli,et al.  Dynamics of beta-cell turnover: evidence for beta-cell turnover and regeneration from sources of beta-cells other than beta-cell replication in the HIP rat. , 2009, American journal of physiology. Endocrinology and metabolism.

[13]  Ralph A. DeFronzo,et al.  From the Triumvirate to the Ominous Octet: A New Paradigm for the Treatment of Type 2 Diabetes Mellitus , 2009, Diabetes.

[14]  C. Sempoux,et al.  Pancreatic β‐cell mass in European subjects with type 2 diabetes , 2008, Diabetes, obesity & metabolism.

[15]  P. Butler,et al.  The replication of β cells in normal physiology, in disease and for therapy , 2007, Nature Clinical Practice Endocrinology &Metabolism.

[16]  Victor Couture,et al.  Appendix for online publication , 2007 .

[17]  R. Rizza,et al.  Modestly increased beta cell apoptosis but no increased beta cell replication in recent-onset type 1 diabetic patients who died of diabetic ketoacidosis , 2007, Diabetologia.

[18]  L. Kuller,et al.  Japanese men have larger areas of visceral adipose tissue than Caucasian men in the same levels of waist circumference in a population-based study , 2006, International Journal of Obesity.

[19]  R. Rizza,et al.  Sustained beta cell apoptosis in patients with long-standing type 1 diabetes: indirect evidence for islet regeneration? , 2005, Diabetologia.

[20]  R. Goebbels,et al.  Cellular composition of the human diabetic pancreas , 1983, Diabetologia.

[21]  I. Naruse,et al.  Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis , 2004, Histochemistry.

[22]  Jae-Hyoung Cho,et al.  Selective β-Cell Loss and α-Cell Expansion in Patients with Type 2 Diabetes Mellitus in Korea , 2003 .

[23]  Y. Ohashi,et al.  Obesity and type 2 diabetes in Japanese patients , 2003, The Lancet.

[24]  Robert A. Rizza,et al.  β-Cell Deficit and Increased β-Cell Apoptosis in Humans With Type 2 Diabetes , 2003, Diabetes.

[25]  P. Zimmet,et al.  Criteria and classification of obesity in Japan and Asia‐Oceania , 2002 .

[26]  M. Cnop,et al.  Beta-cell function is a major contributor to oral glucose tolerance in high-risk relatives of four ethnic groups in the U.S. , 2002, Diabetes.

[27]  S. Yagihashi,et al.  Reduced beta-cell mass and expression of oxidative stress-related DNA damage in the islet of Japanese Type II diabetic patients , 2002, Diabetologia.

[28]  J. Shaw,et al.  Global and societal implications of the diabetes epidemic , 2001, Nature.

[29]  G A Colditz,et al.  Impact of overweight on the risk of developing common chronic diseases during a 10-year period. , 2001, Archives of internal medicine.

[30]  T. Gill.  Cardiovascular risk in the Asia-Pacific region from a nutrition and metabolic point of view: abdominal obesity. , 2001, Asia Pacific journal of clinical nutrition.

[31]  E. Sasaki,et al.  Pancreatic volume in type 1 und type 2 diabetes mellitus , 2001, Acta Diabetologica.

[32]  T. Sugiyama,et al.  Detection of Apoptotic Cells in Human Colorectal Cancer by Two Different in situ Methods: Antibody against Single‐stranded DNA and Terminal Deoxynucleotidyl Transferase‐mediated dUTP‐biotin Nick End‐labeling (TUNEL) Methods , 1999, Japanese journal of cancer research : Gann.

[33]  T. Sugiyama,et al.  Antibody against single-stranded DNA useful for detecting apoptotic cells recognizes hexadeoxynucleotides with various base sequences. , 1998, Journal of biochemistry.

[34]  I. Ijiri,et al.  A statistical analysis of the internal organ weights of normal Japanese people. , 1997, Health physics.

[35]  G. Colditz,et al.  Weight Gain as a Risk Factor for Clinical Diabetes Mellitus in Women , 1995, Annals of Internal Medicine.

[36]  K. Polonsky,et al.  Twenty-four-hour profiles and pulsatile patterns of insulin secretion in normal and obese subjects. , 1988, The Journal of clinical investigation.

[37]  R. Bergman,et al.  Physiologic evaluation of factors controlling glucose tolerance in man: measurement of insulin sensitivity and beta-cell glucose sensitivity from the response to intravenous glucose. , 1981, The Journal of clinical investigation.