Comparison of the nucleic acid sequence of anglerfish and mammalian insulin mRNA's from cloned cDNA's.

Anglerfish (Lophius americanus) insulin complementary DNA was cloned in bacterial plasmids, and its sequence was determined. Fish insulin messenger RNA is larger (1.5 times) than the messenger RNA encoding mammalian (rat and human) insulin, in part because of a larger C peptide (an additional six amino acids or 18 nucleotides in length) but mainly because of increases in the 5' and 3' untranslated regions. Comparison of the fish, rat, and human insulin messenger RNA (from the complementary DNA) reveals that, in addition to the regions coding for the A and B peptides, sequence conservation is limited to a segment within the 5' untranslated region which may be involved in ribosomal binding, two small segments of the signal peptide, and two stretches of sequence in the 3' untranslated region.

[1]  J. Martial,et al.  Structure of cloned DNA complementary to rat prolactin messenger RNA. , 1980, The Journal of biological chemistry.

[2]  Howard M. Goodman,et al.  Sequence of the human insulin gene , 1980, Nature.

[3]  W. Rutter,et al.  Nucleotide sequence of a cDNA clone encoding human preproinsulin , 1979, Nature.

[4]  Howard M. Goodman,et al.  Isolation, cloning and sequence analysis of the cDNA for the α-subunit of human chorionic gonadotropin , 1979, Nature.

[5]  W. Rutter,et al.  Isolation and characterization of a cloned rat insulin gene , 1979, Cell.

[6]  N. Rosenthal,et al.  The structure and evolution of the two nonallelic rat preproinsulin genes , 1979, Cell.

[7]  Stanley N Cohen,et al.  Nucleotide sequence of cloned cDNA for bovine corticotropin-β-lipotropin precursor , 1979, Nature.

[8]  P. Lomedico,et al.  A bacterial clone synthesizing proinsulin. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Steitz,et al.  Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells , 1978, Cell.

[10]  Peter H. Seeburg,et al.  Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone , 1977, Nature.

[11]  P. Seeburg,et al.  Construction and analysis of recombinant DNA for human chorionic somatomammotropin , 1977, Nature.

[12]  W. Rutter,et al.  Rat insulin genes: construction of plasmids containing the coding sequences. , 1977, Science.

[13]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[14]  G. Blobel,et al.  Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[15]  I. Tinoco,et al.  Stability of ribonucleic acid double-stranded helices. , 1974, Journal of molecular biology.

[16]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.

[17]  N. Davidson,et al.  Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. , 1976, Analytical biochemistry.

[18]  J. Martial,et al.  Human growth hormone: complementary DNA cloning and expression in bacteria. , 1979, Science.