Further histochemical aspects of the development of the fetal porcine epidermis.

Summary Histochemical methods for nucleic acids and glycogen were used to study epidermis development from fetal porcine skin in fetuses of 41 days of gestation (d GA) until birth. The development of nucleic acid contents as measured densitometrically in the stratum basale reflects structural changes occurring during epidermis differentiation and is also related to epidermal thickness development. Extinction peaks are followed by an increased production of intermediate cells (about 70 d GA), and the first generation of adult-type, keratinizing epidermal cells (about 80–85 d GA), respectively, which form the first thin stratum corneum lamellae below the stratum intermedium. The glycogen content of fetal porcine epidermis likewise develops in close connection with structural maturation, i. e. it is highest in peridermal and intermediate cells just before the onset of an increased deposition of cytokeratins in these cells at about 67dGA, and later diminishes until birth. Zusammenfassung Weitere histochemische Aspekte zur Entwicklung der fetalen Epidermis des Schweines Mit histochemischen Methoden zur Darstellung von Nukleinsauren und Glykogen wurde die Entwicklung der fetalen Epidermis des Schweines untersucht (41. Ontogenesetag bis zur Geburt). Wie nach densitometrischer Auswertung festgestellt werden konnte, spiegelt die wechselnde Intensitat der Nukleinsaurenanfarbung im Stratum basale wesentliche strukturelle Veranderungen der fetalen Epidermisentwicklung des Schweines wider. Dies steht einmal in Beziehung zur Dickenentwicklung der fetalen Epidermis, zum anderen folgen den beiden Extinktionsmaxima eine wachsende Produktion von Stratum intermedium-Zellen (um den 70. Ontogenesetag) bzw. die erste Generation typischer, verhornender Zellen der adulten Epidermis (um den 80.–85. Ontogenesetag), die schlieslich die ersten dunnen Stratum corneum-Lamellen bilden. Der Glykogengehalt der fetalen Epidermis des Schweines entwickelt sich ebenfalls in enger Beziehung zur Strukturausreifung, d. h. er ist in den Periderm- und Intermedium-Zellen kurz vor dem Beginn einer gesteigerten Cytokeratinproduktion am hochsten (um den 67. Ontogenesetag) und nimmt zur Geburt hin ab.

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