Genome sequence of Phaeobacter caeruleus type strain (DSM 24564T), a surface-associated member of the marine Roseobacter clade

In 2009 Phaeobacter caeruleus was described as a novel species affiliated with the marine Roseobacter clade, which, in turn, belongs to the class Alphaproteobacteria. The genus Phaeobacter is well known for members that produce various secondary metabolites. Here we report of putative quorum sensing systems, based on the finding of six N-acyl-homoserine lactone synthetases, and show that the blue color of P. caeruleus is probably due to the production of the secondary metabolite indigoidine. Therefore, P. caeruleus might have inhibitory effects on other bacteria. In this study the genome of the type strain DSM 24564T was sequenced, annotated and characterized. The 5,344,419 bp long genome with its seven plasmids contains 5,227 protein-coding genes (3,904 with a predicted function) and 108 RNA genes.

[1]  G. Garrity,et al.  Alphaproteobacteria class. nov. , 2015 .

[2]  Natalia N. Ivanova,et al.  Genome sequence of the Leisingera aquimarina type strain (DSM 24565T), a member of the marine Roseobacter clade rich in extrachromosomal elements , 2013, Standards in genomic sciences.

[3]  Benjamin Hofner,et al.  opm: an R package for analysing OmniLog® phenotype microarray data , 2013, Bioinform..

[4]  J. Ruan [Bergey's Manual of Systematic Bacteriology (second edition) Volume 5 and the study of Actinomycetes systematic in China]. , 2013, Wei sheng wu xue bao = Acta microbiologica Sinica.

[5]  M. Göker,et al.  Extrachromosomal, extraordinary and essential—the plasmids of the Roseobacter clade , 2013, Applied Microbiology and Biotechnology.

[6]  Alexander F. Auch,et al.  Genome sequence-based species delimitation with confidence intervals and improved distance functions , 2013, BMC Bioinformatics.

[7]  Thomas S. Brettin,et al.  The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation , 2012, PloS one.

[8]  H. Brinkmann,et al.  Think pink: photosynthesis, plasmids and the Roseobacter clade. , 2012, Environmental microbiology.

[9]  T. Thomas,et al.  Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life , 2012, The ISME Journal.

[10]  A. Buchan,et al.  Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I , 2012, Applied and Environmental Microbiology.

[11]  Hans-Peter Klenk,et al.  Visualization and Curve-Parameter Estimation Strategies for Efficient Exploration of Phenotype Microarray Kinetics , 2012, PloS one.

[12]  I-Min A. Chen,et al.  The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata , 2011, Nucleic Acids Res..

[13]  G. Garrity Bergey’s Manual® of Systematic Bacteriology , 2012, Springer New York.

[14]  A. B. Soliev,et al.  Bioactive Pigments from Marine Bacteria: Applications and Physiological Roles , 2011, Evidence-based complementary and alternative medicine : eCAM.

[15]  S. Schulz,et al.  Tropodithietic Acid Production in Phaeobacter gallaeciensis Is Regulated by N-Acyl Homoserine Lactone-Mediated Quorum Sensing , 2011, Journal of bacteriology.

[16]  Anton Güntsch,et al.  The DNA bank network: the start from a german initiative. , 2011, Biopreservation and biobanking.

[17]  Jörn Petersen Phylogeny and compatibility: plasmid classification in the genomics era , 2011, Archives of Microbiology.

[18]  H. Brinkmann,et al.  Origin and evolution of a novel DnaA-like plasmid replication type in Rhodobacterales. , 2011, Molecular biology and evolution.

[19]  J. Mougous,et al.  What is type VI secretion doing in all those bugs? , 2010, Trends in microbiology.

[20]  N. Jiao,et al.  Oceanicola nitratireducens sp. nov., a marine alphaproteobacterium isolated from the South China Sea. , 2010, International journal of systematic and evolutionary microbiology.

[21]  S. Giovannoni,et al.  Genome Sequences of Oceanicola granulosus HTCC2516T and Oceanicola batsensis HTCC2597T , 2010, Journal of bacteriology.

[22]  Natalia N. Ivanova,et al.  GenePRIMP: a gene prediction improvement pipeline for prokaryotic genomes , 2010, Nature Methods.

[23]  J. Crawford,et al.  Siderophores from neighboring organisms promote the growth of uncultured bacteria. , 2010, Chemistry & biology.

[24]  Hans-Peter Klenk,et al.  Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison , 2010, Standards in genomic sciences.

[25]  Hans-Peter Klenk,et al.  Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs , 2010, Standards in genomic sciences.

[26]  I-Min A. Chen,et al.  The Genomes On Line Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata , 2007, Nucleic Acids Res..

[27]  Miriam L. Land,et al.  Trace: Tennessee Research and Creative Exchange Prodigal: Prokaryotic Gene Recognition and Translation Initiation Site Identification Recommended Citation Prodigal: Prokaryotic Gene Recognition and Translation Initiation Site Identification , 2022 .

[28]  T. Oh,et al.  Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. , 2009, International journal of systematic and evolutionary microbiology.

[29]  H. Brinkmann,et al.  Diversity and evolution of repABC type plasmids in Rhodobacterales. , 2009, Environmental microbiology.

[30]  Natalia N. Ivanova,et al.  The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes , 2009, Standards in genomic sciences.

[31]  Alexandros Stamatakis,et al.  How Many Bootstrap Replicates Are Necessary? , 2009, RECOMB.

[32]  Z. Shao,et al.  Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. , 2009, International journal of systematic and evolutionary microbiology.

[33]  P. Vandamme,et al.  Phaeobacter caeruleus sp. nov., a blue-coloured, colony-forming bacterium isolated from a marine electroactive biofilm. , 2009, International journal of systematic and evolutionary microbiology.

[34]  P. Vandamme,et al.  Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. , 2008, International journal of systematic and evolutionary microbiology.

[35]  P. de Vos,et al.  Alteromonas genovensis sp. nov., isolated from a marine electroactive biofilm and emended description of Alteromonas macleodii Baumann et al. 1972 (approved lists 1980). , 2008, International journal of systematic and evolutionary microbiology.

[36]  J. Rougemont,et al.  A rapid bootstrap algorithm for the RAxML Web servers. , 2008, Systematic biology.

[37]  Huirong Li,et al.  Phaeobacter arcticus sp. nov., a psychrophilic bacterium isolated from the Arctic. , 2008, International journal of systematic and evolutionary microbiology.

[38]  E. Birney,et al.  Velvet: algorithms for de novo short read assembly using de Bruijn graphs. , 2008, Genome research.

[39]  C. Nusbaum,et al.  ALLPATHS: de novo assembly of whole-genome shotgun microreads. , 2008, Genome research.

[40]  Chris F. Taylor,et al.  The minimum information about a genome sequence (MIGS) specification , 2008, Nature Biotechnology.

[41]  M. Simon,et al.  Diversity, ecology, and genomics of the Roseobacter clade: a short overview , 2008, Archives of Microbiology.

[42]  J. Euzéby Validation List no. 117. List of new names and new combinations previously effectively, but not validly, published. , 2007, International journal of systematic and evolutionary microbiology.

[43]  P. Chang,et al.  Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. , 2007, International journal of systematic and evolutionary microbiology.

[44]  T. Oh,et al.  Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. , 2007, International journal of systematic and evolutionary microbiology.

[45]  R. Barrangou,et al.  CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes , 2007, Science.

[46]  Jun Gu,et al.  Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. , 2007, International journal of systematic and evolutionary microbiology.

[47]  H. Grossart,et al.  Bacteria of the Roseobacter Clade Show Potential for Secondary Metabolite Production , 2007, Microbial Ecology.

[48]  Eoin L. Brodie,et al.  Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB , 2006, Applied and Environmental Microbiology.

[49]  G. Garrity,et al.  Class I. Alphaproteobacteria class. nov. , 2005 .

[50]  Michael P. Cummings,et al.  PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)] , 2004 .

[51]  T. Heidorn,et al.  Antibiotic Production by a Roseobacter Clade-Affiliated Species from the German Wadden Sea and Its Antagonistic Effects on Indigenous Isolates , 2004, Applied and Environmental Microbiology.

[52]  E. Cascales,et al.  The versatile bacterial type IV secretion systems , 2003, Nature Reviews Microbiology.

[53]  K. Goodwin,et al.  Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. , 2002, International journal of systematic and evolutionary microbiology.

[54]  Christopher J. Lee,et al.  Multiple sequence alignment using partial order graphs , 2002, Bioinform..

[55]  D. Swofford PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10 , 2002 .

[56]  U. Zielenkiewicz,et al.  Mechanisms of plasmid stable maintenance with special focus on plasmid addiction systems. , 2001, Acta biochimica Polonica.

[57]  M. Ashburner,et al.  Gene Ontology: tool for the unification of biology , 2000, Nature Genetics.

[58]  Wei Qian,et al.  Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. , 2000, Molecular biology and evolution.

[59]  C. Lambert,et al.  Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus. , 1998, International journal of systematic bacteriology.

[60]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[61]  O. Kandler,et al.  Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[62]  Martin F. Porter,et al.  An algorithm for suffix stripping , 1997, Program.