Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys

The antioxidant activity of two selected Malaysian honeys, as well as their ethyl acetate extracts, were evaluated. The antioxidant activities were determined in terms of their anti radical power (ARP) as assessed by DPPH radical scavenging assay and their total antioxidant power (TAP), as assessed by FRAP assay. Total phenolic content of the extracts was determined according to the Folin-Ciocalteau procedure. The characteristic antioxidant activities showed a marked correlation with the total phenolic contents. These results indicated that honey has antioxidative and radical scavenging properties, which are mainly due to its phenolic content. This is the first report of the antioxidant properties of Malaysian honeys.

[1]  H. Shi,et al.  Antioxidative effect of polyphenol extract prepared from various Chinese teas. , 1992, Preventive medicine.

[2]  E. Feskens,et al.  Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study , 1993, The Lancet.

[3]  F. Tomás-Barberán,et al.  Flavonoid composition of Tunisian honeys and propolis , 1997 .

[4]  R. J. Weston,et al.  Identification and quantitative levels of antibacterial components of some New Zealand honeys. , 2000 .

[5]  S. Y. Wang,et al.  Antioxidant activity and phenolic compounds in selected herbs. , 2001, Journal of agricultural and food chemistry.

[6]  F. Tomás-Barberán,et al.  Plant Phenolic Metabolites and Floral Origin of Rosemary Honey , 1995 .

[7]  B. D. Oomah,et al.  Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products , 1998 .

[8]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[9]  R. Prior,et al.  High-Performance Liquid Chromatography Coupled with Coulometric Array Detection of Electroactive Components in Fruits and Vegetables: Relationship to Oxygen Radical Absorbance Capacity , 1997 .

[10]  K. Kondo,et al.  Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. , 2001, Journal of nutritional science and vitaminology.

[11]  J. Strain,et al.  Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. , 1999, Methods in enzymology.

[12]  H. Gerster Antioxidant vitamins in cataract prevention , 1989, Zeitschrift fur Ernahrungswissenschaft.

[13]  C. Scaccini,et al.  Inhibition of human low-density lipoprotein oxidation by caffeic acid and other hydroxycinnamic acid derivatives. , 1995, Free Radical Biology & Medicine.

[14]  F. Tomás-Barberán,et al.  Flavonoids of “La Alcarria” honey A study of their botanical origin , 1992 .

[15]  R. Prior,et al.  Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. , 1997, Free radical biology & medicine.

[16]  P. Molan The Antibacterial Activity of Honey: 1. The nature of the antibacterial activity , 1992 .

[17]  J. Laranjinha,et al.  Reactivity of dietary phenolic acids with peroxyl radicals: antioxidant activity upon low density lipoprotein peroxidation. , 1994, Biochemical pharmacology.

[18]  O. Çetinkale,et al.  Oxygen Free Radicals Impair Wound Healing in Ischemic Rat Skin , 1997, Annals of plastic surgery.

[19]  J. Vinson,et al.  Plant Flavonoids, Especially Tea Flavonols, Are Powerful Antioxidants Using an in Vitro Oxidation Model for Heart Disease , 1995 .

[20]  J. Warthesen,et al.  Antioxidant activity of nontocopherol hazelnut (Corylus spp.) phenolics. , 2000 .

[21]  A. Schepartz Honey Catalase: Occurrence and Some Kinetic Properties , 1966 .

[22]  M. Berenbaum,et al.  Antioxidant capacity and correlated characteristics of 14 unifloral honeys , 1998 .

[23]  Smirnov Da Acute pancreatitis and biological antioxidants , 1994 .

[24]  M. Berenbaum,et al.  Honeys from different floral sources as inhibitors of enzymatic browning in fruit and vegetable homogenates. , 2000, Journal of agricultural and food chemistry.

[25]  E. Crane Honey. A comprehensive survey , 1978, Medical History.

[26]  C. V. Morr,et al.  Improved high-performance liquid chromatographic analysis of phenolic acids and isoflavonoids from soybean protein products , 1984 .

[27]  F. Tomás-Barberán,et al.  A simple extractive technique for honey flavonoid HPLC analysis , 1994 .