Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study.

Cell-substrate interactions are investigated in a number of studies for drug targets including angiogenesis, arteriosclerosis, chronic inflammatory diseases and carcinogenesis. One characteristic of malignant cancerous cells is their ability to invade tissue. Cell adhesion and cytoskeletal activity have served as valuable indicators for understanding the cancer cell behaviours, such as proliferation, migration and invasion. This review focuses on bio-impedance based measurement for monitoring the behaviours in real time and without using labels. Electric cell-substrate impedance sensing (ECIS) provides rich information about cell-substrate interactions, cell-cell communication and cell adhesion. High sensitivity of the ECIS method allows for observing events down to single-cell level and achieving nanoscale resolution of cell-substrate distances. Recently, its miniaturization and integration with fluorescent detection techniques have been highlighted as a new tool to deliver a high-content platform for anticancer drug development.

[1]  C. Lo,et al.  Distinguishing cancerous from noncancerous cells through analysis of electrical noise. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[2]  A. Malik,et al.  Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Bernard Lachance,et al.  Assessment of cytotoxicity using electric cell-substrate impedance sensing: concentration and time response function approach. , 2002, Analytical chemistry.

[4]  Xiao Xu,et al.  The application of cell‐based label‐free technology in drug discovery , 2008, Biotechnology journal.

[5]  A. Li,et al.  Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. , 1999, Chemico-biological interactions.

[6]  Ivar Giaever,et al.  Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue Culture , 1986, IEEE Transactions on Biomedical Engineering.

[7]  John H T Luong,et al.  Assessment of cytotoxicity by emerging impedance spectroscopy. , 2005, Toxicology and applied pharmacology.

[8]  Ivar Giaever,et al.  Podokinesis in endothelial cell migration: role of nitric oxide. , 1998, American journal of physiology. Cell physiology.

[9]  Ali Khademhosseini,et al.  Microfluidics for drug discovery and development: from target selection to product lifecycle management. , 2008, Drug discovery today.

[10]  Lei Wang,et al.  Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip. , 2010, Biosensors & bioelectronics.

[11]  Ivar Giaever,et al.  A morphological biosensor for mammalian cells , 1993, Nature.

[12]  C. Lo,et al.  Impedance analysis of MDCK cells measured by electric cell-substrate impedance sensing. , 1995, Biophysical journal.

[13]  Ronit Satchi-Fainaro,et al.  Tumor cytotoxicity and endothelial Rac inhibition induced by TNP-470 in anaplastic thyroid cancer , 2007, Molecular Cancer Therapeutics.

[14]  Fred Hirsch,et al.  The aryl hydrocarbon receptor repressor is a putative tumor suppressor gene in multiple human cancers. , 2008, The Journal of clinical investigation.

[15]  Li Feng,et al.  Lysophosphatidic acid is constitutively produced by human peritoneal mesothelial cells and enhances adhesion, migration, and invasion of ovarian cancer cells. , 2006, Cancer research.

[16]  Ali H. Brivanlou,et al.  Signaling Pathways in Cancer and Embryonic Stem Cells , 2007, Stem Cell Reviews.

[17]  W. Jiang,et al.  Placenta growth factor, PLGF, influences the motility of lung cancer cells, the role of Rho associated kinase, Rock1 , 2008, Journal of cellular biochemistry.

[18]  Guillermo Repetto,et al.  Neutral red uptake assay for the estimation of cell viability/cytotoxicity , 2008, Nature Protocols.

[19]  Ivar Giaever,et al.  Permissive Role of Nitric Oxide in Endothelin-induced Migration of Endothelial Cells* , 1997, The Journal of Biological Chemistry.

[20]  J. W. Parce,et al.  Detection of cell-affecting agents with a silicon biosensor. , 1989, Science.

[21]  I. Giaever,et al.  Assessment of rapid morphological changes associated with elevated cAMP levels in human orbital fibroblasts. , 1998, Experimental cell research.

[22]  Ju Hun Yeon,et al.  Cytotoxicity test based on electrochemical impedance measurement of HepG2 cultured in microfabricated cell chip. , 2005, Analytical biochemistry.

[23]  Andrew J deMello,et al.  Micro- and nanofluidic systems for high-throughput biological screening. , 2009, Drug discovery today.

[24]  Xiao Xu,et al.  Dynamic and label-free cell-based assays using the real-time cell electronic sensing system. , 2006, Assay and drug development technologies.

[25]  N. Thomas,et al.  Stealth sensors: real-time monitoring of the cell cycle , 2003 .

[26]  T. Hug,et al.  Biophysical methods for monitoring cell-substrate interactions in drug discovery. , 2003, Assay and drug development technologies.

[27]  Didier Merlin,et al.  Concomitant activation of the JAK/STAT, PI3K/AKT, and ERK signaling is involved in leptin-mediated promotion of invasion and migration of hepatocellular carcinoma cells. , 2007, Cancer research.

[28]  Chie-Pein Chen,et al.  Transforming growth factor-beta induces CD44 cleavage that promotes migration of MDA-MB-435s cells through the up-regulation of membrane type 1-matrix metalloproteinase. , 2009, International journal of cancer.

[29]  S I Simon,et al.  Analysis of tight junctions during neutrophil transendothelial migration. , 2000, Journal of cell science.

[30]  J. Wegener,et al.  Electric cell-substrate impedance sensing (ECIS) as a noninvasive means to monitor the kinetics of cell spreading to artificial surfaces. , 2000, Experimental cell research.

[31]  I. Giaever,et al.  Micromotion of mammalian cells measured electrically. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[32]  W. H. van der Schalie,et al.  Improved cell sensitivity and longevity in a rapid impedance‐based toxicity sensor , 2009, Journal of applied toxicology : JAT.

[33]  YeoHeung Yun,et al.  Development of an electrode cell impedance method to measure osteoblast cell activity in magnesium-conditioned media , 2010, Analytical and bioanalytical chemistry.

[34]  John H T Luong,et al.  On‐Line Monitoring of Cell Growth and Cytotoxicity Using Electric Cell‐Substrate Impedance Sensing (ECIS) , 2003, Biotechnology progress.

[35]  W. Jiang,et al.  The prostate transglutaminase (TGase-4, TGaseP) regulates the interaction of prostate cancer and vascular endothelial cells, a potential role for the ROCK pathway. , 2009, Microvascular research.

[36]  E Wintermantel,et al.  Feasibility study of an online toxicological sensor based on the optical waveguide technique. , 2000, Biosensors & bioelectronics.

[37]  Andreas Janshoff,et al.  Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis. , 2010, Integrative biology : quantitative biosciences from nano to macro.

[38]  Giljun Park,et al.  Electrical impedance measurements predict cellular transformation , 2009, Cell biology international.

[39]  Joachim Wegener,et al.  Electrical wound-healing assay for cells in vitro. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[40]  S I Simon,et al.  Neutrophil transendothelial migration is independent of tight junctions and occurs preferentially at tricellular corners. , 1997, Journal of immunology.

[41]  Heinrich Leonhardt,et al.  Cell Cycle Markers for Live Cell Analyses , 2005, Cell cycle.

[42]  Chun-Min Lo,et al.  Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity. , 2009, Biosensors & bioelectronics.

[43]  R. E. White,et al.  High-throughput screening in drug metabolism and pharmacokinetic support of drug discovery. , 2000, Annual review of pharmacology and toxicology.

[44]  I. Giaever,et al.  Monitoring fibroblast behavior in tissue culture with an applied electric field. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[45]  D. Lawrence,et al.  Measurement of macrophage adherence and spreading with weak electric fields. , 1990, Journal of immunological methods.

[46]  C. Lo,et al.  Electrically measuring viscoelastic parameters of adherent cell layers under controlled magnetic forces , 1999, European Biophysics Journal.

[47]  W. Zhang,et al.  The signaling network of tumor invasion. , 2005, Histology and histopathology.

[48]  C. Lieber,et al.  Toxicity of β-carotene and its exacerbation by acetaldehyde in HepG2 cells , 2001 .

[49]  J. Hauser,et al.  Autocrine Transforming Growth Factor α Regulates Cell Adhesion by Multiple Signaling via Specific Phosphorylation Sites of p70S6 Kinase in Colon Cancer Cells* , 2004, Journal of Biological Chemistry.