Inactivation Kinetics of Foodborne Spoilage and Pathogenic Bacteria by Ozone

: Ozone was tested against Pseudomonas fluorescens, Escherichia coli O157:H7, Leuconostoc mesenteroides, and Listeria monocytogenes. When kinetic data from a batch reactor were fitted to a dose-response model, a 2-phased linear relationship was observed. A continuous ozone reactor was developed to ensure a uniform exposure of bacterial cells to ozone and a constant concentration of ozone during the treatment. Survivors plots in the continuous system were linear initially, followed by a concave downward pattern. Exposure of bacteria to ozone at 2.5 ppm for 40 s caused 5 to 6 log decrease in count. Resistance of tested bacteria to ozone followed this descending order: E. coli O157:H7, P. fluorescens, L. mesenteroides, and L. monocytogenes.

[1]  D B SCOTT,et al.  EFFECT OF OZONE ON SURVIVAL AND PERMEABILITY OF ESCHERICHIA COLI , 1963, Journal of bacteriology.

[2]  Jian Zhao,et al.  Microbial decontamination of black pepper by ozone and the effect of the treatment on volatile oil constituents of the spice , 1995 .

[3]  E. Katzenelson,et al.  Inactivation Kinetics of Viruses and Bacteria in Water by Use of Ozone , 1974 .

[4]  Benito Jose Marinas,et al.  Kinetics of Escherichia coli inactivation with ozone , 1997 .

[5]  George Rempel,et al.  Ozone for Supplementary Water Treatment , 1972 .

[6]  A. Yousef,et al.  Resistance of Listeria monocytogenes to Heat after Adaptation to Environmental Stresses. , 1996, Journal of food protection.

[7]  Ahmed E. Yousef,et al.  Use of ozone to inactivate microorganisms on lettuce , 1999 .

[8]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater. 14th edition. , 1976 .

[9]  H. Shechter.  Spectrophotometric method for determination of ozone in aqueous solutions , 1973 .

[10]  P. Edelstein,et al.  Efficacy of ozone in eradication of Legionella pneumophila from hospital plumbing fixtures , 1982, Applied and environmental microbiology.

[11]  C. Gomella.  Ozone Practices in France , 1972 .

[12]  Daniel W. Smith,et al.  Dose—response of Escherichia coli in ozone demand-free phosphate buffer , 1988 .

[13]  J. Hoigne,et al.  Determination of ozone in water by the indigo method , 1981 .

[14]  D. Crook.  Proceedings of the Pathological Society of Great Britain and Ireland. 166th meeting. London, 6-8 January 1993. Abstracts. , 1995, Journal of medical microbiology.

[15]  N. A. Sinclair,et al.  Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability , 1990, Applied and environmental microbiology.

[16]  A. Yousef,et al.  Application of ozone for enhancing the microbiological safety and quality of foods: a review. , 1999, Journal of food protection.

[17]  S. Farooq,et al.  Comparative response of mixed cultures of bacteria and virus to ozonation , 1983 .

[18]  W T Broadwater,et al.  Sensitivity of three selected bacterial species to ozone. , 1973, Applied microbiology.

[19]  E. R. Baumann,et al.  Free Available Chlorine Residuals for Small Nonpublic Water Supplies , 1962 .

[20]  J. Joret,et al.  Wastewater Disinfection : Elimination of Fecal Bacteria and Eneric Viruses By Ozone , 1982 .

[21]  R. S. Engelbrecht,et al.  Basic concepts in disinfection with ozone. , 1977, Journal - Water Pollution Control Federation.

[22]  R. Rice,et al.  Handbook of ozone technology and applications , 1982 .

[23]  D. S. Boyce,et al.  The effect of bentonite clay on ozone disinfection of bacteria and viruses in water , 1981 .

[24]  E. W. Frampton,et al.  Efficacy of ozonated water against various food-related microorganisms , 1995, Applied and environmental microbiology.