Fast‐Transported Glycoproteins and Nonglycosylated Proteins Contain Sulfate

Abstract: 35SO4‐labeled fast‐transported proteins of bullfrog dorsal root ganglion neurons were separated by two‐dimensional gel electrophoresis, and their mobilities were compared to similar species labeled with [3H]mannose or [3H]fucose. Fluorography revealed regions of poorly resolved, high molecular weight material, likely to represent sulfated proteoglycans, as well as many well resolved spots that corresponded in mobility to individual [35S]methionine‐labeled fast‐transported proteins. The majority of these well resolved spots appeared as “families,” previously identified as glycoproteins based on their labeling with sugars. Thus, sulfate can be a contributor to the carbohydrate side‐chain charge that underlies microheterogeneity. The most heavily 35SO4‐labeled species, however, corresponded to fast‐transported proteins that were not labeled by either sugar. The relative acid labilities of 35SO4 associated with individual species cut from the gel confirmed the assignments of these spots as glycoproteins or nonglycoproteins. A group of spots intermediate in their acid lability was also detected, suggesting that some proteins may contain sulfate linked to carbohydrate as well as to amino acid residues.