Identification of Hsa_circ_0104824 as a Potential Biomarkers for Breast Cancer

Background: To study the specific expression of circular RNA (circRNA) in breast cancer and adjacent normal tissues to identify differentially expressed circRNA in breast cancer patients. To study the correlation between circRNA and clinical data of breast cancer, and to evaluate its potential as a breast cancer biomarker. Methods: The differential expression of circRNAs between the breast cancer tissues and adjacent normal tissues was screened by Human CircRNA Microarray. Candidate circRNAs verified by qRT-PCR. CircRNA was analyzed by Agilent GeneSpring 13.0 software. SPSS23.0, GraphPad Prism, and Sigmaplot software were used for statistical analysis. Perform T test, one-way ANOVA, curve regression analysis and ROC curve analysis for evaluating the diagnostic value of circular RNA. Results: Among the 2021 differentially expressed circRNAs, 546 were up-regulated and 1475 were down-regulated in breast cancer tissues. The validation study demonstrated that six circRNAs were downregulated. Among them, hsa_circ_0104824 proved high correlation between breast cancer tissues and plasma samples in its expression and diagnostic value. Conclusion: Hsa_circ_0104824 may serve as a promising predictive biomarker and therapeutic target for patients with breast cancer.

[1]  D. Romagnolo,et al.  Dietary fat and obesity as modulators of breast cancer risk: Focus on DNA methylation , 2020, British journal of pharmacology.

[2]  Zhenjie Wu,et al.  CircRNA cRAPGEF5 inhibits the growth and metastasis of renal cell carcinoma via the miR-27a-3p/TXNIP pathway. , 2020, Cancer letters.

[3]  Mingchao Wang,et al.  Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals , 2019, Molecular Cancer.

[4]  Zhongfeng Wang,et al.  Downregulation of circRNA DMNT3B contributes to diabetic retinal vascular dysfunction through targeting miR-20b-5p and BAMBI , 2019, EBioMedicine.

[5]  A. Tiwari,et al.  Novel and Alternative Targets Against Breast Cancer Stemness to Combat Chemoresistance , 2019, Front. Oncol..

[6]  Jianju Liu,et al.  Upregulated circRNA ARHGAP10 Predicts an Unfavorable Prognosis in NSCLC through Regulation of the miR-150-5p/GLUT-1 Axis , 2019, Molecular therapy. Nucleic acids.

[7]  Hailin Tang,et al.  circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer , 2019, Molecular therapy. Nucleic acids.

[8]  Wang Sq,et al.  CircRNA_100876 promote proliferation and metastasis of breast cancer cells through adsorbing microRNA-361-3p in a sponge form. , 2019 .

[9]  Jian-ying Ma,et al.  Identification of circRNA–miRNA–mRNA regulatory network in gastric cancer by analysis of microarray data , 2019, Cancer Cell International.

[10]  Qi Zhang,et al.  Translation of the circular RNA circβ-catenin promotes liver cancer cell growth through activation of the Wnt pathway , 2019, Genome Biology.

[11]  C.-Y. Yang,et al.  CircRNA_100876 promote proliferation and metastasis of breast cancer cells through adsorbing microRNA-361-3p in a sponge form. , 2019, European review for medical and pharmacological sciences.

[12]  Hao Xu,et al.  Regulatory network of circRNA–miRNA–mRNA contributes to the histological classification and disease progression in gastric cancer , 2018, Journal of Translational Medicine.

[13]  Cuntao Lu,et al.  Circular RNA hsa_circ_0008039 promotes breast cancer cell proliferation and migration by regulating miR-432-5p/E2F3 axis. , 2018, Biochemical and biophysical research communications.

[14]  F. Schellevis,et al.  Incidence of isolated local breast cancer recurrence and contralateral breast cancer: A systematic review. , 2018, Breast.

[15]  Jie He,et al.  Health-related quality of life and utility scores of patients with breast neoplasms in China: A multicenter cross-sectional survey. , 2018, Breast.

[16]  Dongwei Zhang,et al.  CircRNA circ_0067934 promotes tumor growth and metastasis in hepatocellular carcinoma through regulation of miR-1324/FZD5/Wnt/β-catenin axis. , 2018, Biochemical and biophysical research communications.

[17]  Lin Guo,et al.  Microarray profile of circular RNAs identifies hsa_circ_0014130 as a new circular RNA biomarker in non-small cell lung cancer , 2018, Scientific Reports.

[18]  J. Kjems,et al.  Circular RNAs in cancer: opportunities and challenges in the field , 2017, Oncogene.

[19]  Yanting Shi,et al.  circHIPK3 regulates cell proliferation and migration by sponging miR-124 and regulating AQP3 expression in hepatocellular carcinoma , 2018, Cell Death & Disease.

[20]  Weigang Wang,et al.  The diagnostic value of serum tumor markers CEA, CA19-9, CA125, CA15-3, and TPS in metastatic breast cancer. , 2017, Clinica chimica acta; international journal of clinical chemistry.

[21]  Minghua Wu,et al.  CircRNA: functions and properties of a novel potential biomarker for cancer , 2017, Molecular Cancer.

[22]  Bin Li,et al.  Circular RNAs in cancer: an emerging key player , 2017, Journal of Hematology & Oncology.

[23]  Alissa M. Weaver,et al.  Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes , 2016, Scientific Reports.

[24]  Rogerio Margis,et al.  Circular RNAs are miRNA sponges and can be used as a new class of biomarker. , 2016, Journal of biotechnology.