Simultaneous Determination of Dopamine, Serotonin and Ascorbic Acid at a Glassy Carbon Electrode Modified with Carbon-Spheres

A novel glassy carbon electrode (GCE) modified with carbon-spheres has been fabricated through a simple casting procedure. The modified GCE displays high selectivity and excellent electrochemical catalytic activities towards dopamine (DA), serotonin (5-HT), and ascorbic acid (AA). In the co-existence system, the peak separations between AA and DA, DA and 5-HT, and AA and 5-HT are large up to 230, 180, and 410 mV, respectively. Differential pulse voltammetry (DPV) has been employed to simultaneously detect DA, 5-HT, and AA, and the linear calibration curves for DA, 5-HT, and AA are obtained in the range of 20.0-150.0 μM, 40.0-750.0 μM and 300.0-2,000.0 μM with detection limits (S/N = 3) of 2.0 μM, 0.7 μM and 0.6 μM, respectively. The proposed electrode has been applied to detect DA, 5-HT, and AA in real samples using standard addition method with satisfactory results.

[1]  Yan Wang,et al.  Enhanced sensing of ascorbic acid, dopamine and serotonin at solid carbon paste electrode with a nonionic polymer film. , 2010, Talanta.

[2]  Xiangqin Lin,et al.  Novel choline and acetylcholine modified glassy carbon electrodes for simultaneous determination of dopamine, serotonin and ascorbic acid , 2004 .

[3]  F. Gao,et al.  Electrochemical Detection of Low-Molecular-Mass Biothiols in Biological Fluids at Carbon Spheres-Modified Glassy Carbon Electrodes , 2013, Electrocatalysis.

[4]  E. D. de Vries,et al.  Profiling of tryptophan-related plasma indoles in patients with carcinoid tumors by automated, on-line, solid-phase extraction and HPLC with fluorescence detection. , 2001, Clinical chemistry.

[5]  R. Wightman,et al.  Electrochemical monitoring of biogenic amine neurotransmission in real time. , 1999, Journal of pharmaceutical and biomedical analysis.

[6]  Boy Hoyer,et al.  Stabilization of the voltammetric serotonin signal by surfactants , 2006 .

[7]  M. Rice.  Ascorbate regulation and its neuroprotective role in the brain , 2000, Trends in Neurosciences.

[8]  R. Ramaraj,et al.  Electrochemically deposited nanostructured platinum on Nafion coated electrode for sensor applications , 2005 .

[9]  B. Ogorevc,et al.  Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly(1,2-phenylenediamine)-coated carbon fiber. , 2001, Analytical chemistry.

[10]  Michael P Marsh,et al.  A carbon nanofiber based biosensor for simultaneous detection of dopamine and serotonin in the presence of ascorbic acid. , 2013, Biosensors & bioelectronics.

[11]  R. Kennedy,et al.  Review of recent advances in analytical techniques for the determination of neurotransmitters. , 2009, Analytica chimica acta.

[12]  Liping Lu,et al.  Attachment of DNA to the Carbon Fiber Microelectrode via Gold Nanoparticles for Simultaneous Determination of Dopamine and Serotonin , 2004, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[13]  R. Wightman,et al.  Detection of dopamine dynamics in the brain. , 1988, Analytical chemistry.

[14]  M. El‐Kady,et al.  Novel poly(3-methylthiophene)/Pd, Pt nanoparticle sensor: Synthesis, characterization and its application to the simultaneous analysis of dopamine and ascorbic acid in biological fluids , 2010 .

[15]  J. B. Justice,et al.  Peer Reviewed: Probing Brain Chemistry: Voltammetry Comes of Age , 1996 .

[16]  R. Compton,et al.  A simple electroanalytical methodology for the simultaneous determination of dopamine, serotonin and ascorbic acid using an unmodified edge plane pyrolytic graphite electrode , 2007, Analytical and bioanalytical chemistry.

[17]  R. Ramaraj,et al.  Simultaneous determination of dopamine and serotonin in the presence of ascorbic acid and uric acid at poly(o-phenylenediamine) modified electrode , 2003 .

[18]  S. Yasui,et al.  Simultaneous voltammetric measurement of nitrite ion, dopamine, serotonin with ascorbic acid on the GRC electrode , 1999 .

[19]  Fabrication of Porous Carbon Spheres/Manganese Oxide Composite Electrodes for Electrochemical Capacitors , 2012 .

[20]  R. Ramaraj,et al.  Simultaneous determination of ascorbic acid, dopamine and serotonin at poly(phenosafranine) modified electrode , 2003 .

[21]  F. Bloom,et al.  The biochemical basis of neuropharmacology, 2nd ed. , 1974 .

[22]  F. Gao,et al.  An electrochemical capacitor electrode based on porous carbon spheres hybrided with polyaniline and nanoscale ruthenium oxide. , 2012, ACS applied materials & interfaces.

[23]  J. B. Justice,et al.  Probing brain chemistry : Voltammetry comes of age , 1996 .

[24]  Lun Wang,et al.  Electrocatalytic activity of carbon spheres towards NADH oxidation at low overpotential and its applications in biosensors and biofuel cells , 2011 .

[25]  Martin Arundell,et al.  Subsecond voltammetric separation between dopamine and serotonin in the presence of ascorbate. , 2006, Analytical chemistry.

[26]  Shengshui Hu,et al.  Simultaneous determination of dopamine and serotonin on a glassy carbon electrode coated with a film of carbon nanotubes. , 2003, Analytical biochemistry.

[27]  R. Adams,et al.  Probing brain chemistry with electroanalytical techniques. , 1976, Analytical chemistry.

[28]  Qionglin Liang,et al.  Carbon nanotube-intercalated graphite electrodes for simultaneous determination of dopamine and serotonin in the presence of ascorbic acid , 2003 .

[29]  J. Oni,et al.  Simultaneous voltammetric determination of dopamine and serotonin on carbon paste electrodes modified with iron(II) phthalocyanine complexes , 2001 .

[30]  Xiangqin Lin,et al.  Overoxidized polypyrrole film directed DNA immobilization for construction of electrochemical micro-biosensors and simultaneous determination of serotonin and dopamine , 2005 .