Induction of Rice Cytosolic Ascorbate Peroxidase mRNA by Oxidative Stress; the Involvement of Hydrogen Peroxide in Oxidative Stress Signalling

The oxidative stress response of rice cytosolic ascorbate peroxidase (APX) was examined. The transcript level of cytosolic APX was significantly increased when suspension cultures of germinating rice embryos were treated with paraquat (7.9-fold) or H 2O: (6.1-fold). Induction by paraquat reached a maximum at 8 h. Induction by H2O2 peaked earlier at 4 h of treatment. This result suggests that the induction by paraquat might be caused by the H2O2 generated from superoxide. Treatment with a superoxide dismutase inhibitor, diethyldithiocarbamate, which is supposed to decrease the cellular H2O2 level, reduced the paraquat induction of cytosolic APX. In contrast, when APX and catalase were inhibited by hydroxyurea or aminotriazole, cellular H2O2 content was elevated and cytosolic APX mRNA was markedly increased without paraquat or H2O2 treatment. This suggests that cytosolic APX is regulated by the H2O2 level within cells. An increase in H 2O2 content was observed in the paraquat treated embryos, suggesting that paraquat induction of cytosolic APX was caused by H 2O2 generated through superoxide dismutation. These results indicate that H2O2 is involved in oxidative stress signalling, leading to the induction of cytosolic APX.

[1]  D. Inzé,et al.  The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter. , 1998, Plant physiology.

[2]  J. Dat,et al.  Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings , 1998, Plant physiology.

[3]  F. Turano,et al.  Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli , 1997, Planta.

[4]  B. Ellis,et al.  Transgenic tobacco plants expressing antisense RNA for cytosolic ascorbate peroxidase show increased susceptibility to ozone injury , 1997 .

[5]  H. Jespersen,et al.  From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase. , 1997, The Biochemical journal.

[6]  W. Van Camp,et al.  Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants , 1997, The EMBO journal.

[7]  J. Dat,et al.  Hydrogen peroxide‐ and glutathione‐associated mechanisms of acclimatory stress tolerance and signalling , 1997 .

[8]  B. Touraine,et al.  Glutathione-Mediated Regulation of ATP Sulfurylase Activity, SO42- Uptake, and Oxidative Stress Response in Intact Canola Roots , 1997, Plant physiology.

[9]  L. A. Río,et al.  Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves , 1997, Plant physiology.

[10]  P. Mullineaux,et al.  Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. , 1997, The Plant cell.

[11]  B. Ellis,et al.  Pre-activating wounding response in tobacco prior to high-level ozone exposure prevents necrotic injury. , 1997, The Plant journal : for cell and molecular biology.

[12]  C. Cramer,et al.  Responses of Antioxidants to Paraquat in Pea Leaves (Relationships to Resistance) , 1997, Plant physiology.

[13]  G. Vanlerberghe,et al.  Signals Regulating the Expression of the Nuclear Gene Encoding Alternative Oxidase of Plant Mitochondria , 1996, Plant physiology.

[14]  G. Paliyath,et al.  Ultraviolet-B- and Ozone-Induced Biochemical Changes in Antioxidant Enzymes of Arabidopsis thaliana , 1996, Plant physiology.

[15]  R. Last,et al.  Differential Accumulation of Antioxidant mRNAs in Arabidopsis thaliana Exposed to Ozone , 1995, Plant physiology.

[16]  M. Nishimura,et al.  A novel isoenzyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin. , 1995, Plant & cell physiology.

[17]  S. Shigeoka,et al.  Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach , 1995, FEBS letters.

[18]  F. J. Corpas,et al.  Salt-induced oxidative stress in chloroplasts of pea plants , 1995 .

[19]  Alex Levine,et al.  H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response , 1994, Cell.

[20]  W. Van Camp,et al.  Ozone, Sulfur Dioxide, and Ultraviolet B Have Similar Effects on mRNA Accumulation of Antioxidant Genes in Nicotiana plumbaginifolia L , 1994, Plant physiology.

[21]  M. Mehdy.  Active Oxygen Species in Plant Defense against Pathogens , 1994, Plant physiology.

[22]  T. K. Prasad,et al.  Acclimation, Hydrogen Peroxide, and Abscisic Acid Protect Mitochondria against Irreversible Chilling Injury in Maize Seedlings , 1994, Plant physiology.

[23]  R. Mittler,et al.  Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. , 1994, The Plant journal : for cell and molecular biology.

[24]  T. K. Prasad,et al.  Evidence for Chilling-Induced Oxidative Stress in Maize Seedlings and a Regulatory Role for Hydrogen Peroxide. , 1994, The Plant cell.

[25]  D. Klessig,et al.  Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. , 1993, Science.

[26]  A. Holaday,et al.  Overexpression of Superoxide Dismutase Protects Plants from Oxidative Stress (Induction of Ascorbate Peroxidase in Superoxide Dismutase-Overexpressing Plants) , 1993, Plant physiology.

[27]  S. Shigeoka,et al.  Hydrogen peroxide generation in organelles of Euglena gracilis , 1993 .

[28]  Neelam P. Mishra,et al.  Changes in the Activities of Anti-Oxidant Enzymes during Exposure of Intact Wheat Leaves to Strong Visible Light at Different Temperatures in the Presence of Protein Synthesis Inhibitors , 1993, Plant physiology.

[29]  P. Baeuerle,et al.  H2O2 and antioxidants have opposite effects on activation of NF‐kappa B and AP‐1 in intact cells: AP‐1 as secondary antioxidant‐responsive factor. , 1993, The EMBO journal.

[30]  H. Tsuji,et al.  Development of the O2--Detoxification System during Adaptation to Air of Submerged Rice Seedlings , 1992 .

[31]  R. Mittler,et al.  The Journal of Biological Chemistry Molecular Cloning and Characterization of a Gene Encoding Pea Cytosolic Ascorbate Peroxidase* , 1992 .

[32]  G. Pastori,et al.  Oxidative Stress Induces High Rate of Glutathione Reductase Synthesis in a Drought-Resistant Maize Strain , 1992 .

[33]  K. Asada,et al.  Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts and Photoreduction of Its Primary Oxidation Product Monodehydroascorbate Radicals in Thylakoids , 1992 .

[34]  K. Asada.  Ascorbate peroxidase – a hydrogen peroxide‐scavenging enzyme in plants , 1992 .

[35]  Y. Matsuda,et al.  Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment. , 1991, Plant physiology.

[36]  P. Baeuerle,et al.  Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF‐kappa B transcription factor and HIV‐1. , 1991, The EMBO journal.

[37]  T. Rushmore,et al.  The antioxidant responsive element. Activation by oxidative stress and identification of the DNA consensus sequence required for functional activity. , 1991, The Journal of biological chemistry.

[38]  K. Asada,et al.  Hydroxyurea and p-aminophenol are the suicide inhibitors of ascorbate peroxidase. , 1990, The Journal of biological chemistry.

[39]  T. Murphy,et al.  Hydrogen peroxide formation in cultured rose cells in response to UV-C radiation. , 1990 .

[40]  N. Kondo,et al.  O3 Tolerance and the Ascorbate-Dependent H2O2 Decomposing System in Chloroplasts , 1985 .

[41]  B. Ames,et al.  Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium , 1985, Cell.

[42]  A. Polidoros,et al.  Catalases in Plants: Gene Structure, Properties, Regulation, and Expression , 1997 .

[43]  Z. Kiraly,et al.  Effects of powdery mildew infection of barley on the ascorbate-glutathione cycle and other antioxidants in different host-pathogen interactions , 1995 .

[44]  Dirk Inzé,et al.  SUPEROXIDE DISMUTASE AND STRESS TOLERANCE , 1992 .

[45]  G. Laties,et al.  Quantification of hydrogen peroxide in plant extracts by the chemiluminescence reaction with luminol , 1982 .