Quantitative retinal-blood flow measurement with three-dimensional vessel geometry determination using ultrahigh-resolution Doppler optical coherence angiography.

Retinal blood flow quantification by retinal vessel segmentation with Doppler optical coherence angiography is presented. Vessel diameter, orientation, and position are determined in an en face vessel image and two representative cross-sectional flow images of the vessel. Absolute blood flow velocity is calculated with the help of the measured Doppler frequency shift and determined vessel angle. The volumetric flow rate is obtained with the position and the region of the vessel lumen. The volumetric blood flow rate of retinal arteries before and after a bifurcation is verified in a healthy human eye.

[1]  Toyohiko Yatagai,et al.  Three-dimensional visualization of choroidal vessels by using standard and ultra-high resolution scattering optical coherence angiography. , 2007, Optics express.

[2]  David Huang,et al.  Measurement of absolute flow velocity vector using dual-angle, delay-encoded Doppler optical coherence tomography. , 2007, Optics letters.

[3]  R. Leitgeb,et al.  Resonant Doppler flow imaging and optical vivisection of retinal blood vessels. , 2007, Optics express.

[4]  Guido Gerig,et al.  Multiscale detection of curvilinear structures in 2-D and 3-D image data , 1995, Proceedings of IEEE International Conference on Computer Vision.

[5]  Teresa C. Chen,et al.  In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical Doppler tomography , 2003 .

[6]  Woonggyu Jung,et al.  Quantification of a three-dimensional velocity vector using spectral-domain Doppler optical coherence tomography. , 2007, Optics letters.

[7]  T. Yatagai,et al.  Optical coherence angiography. , 2006, Optics express.

[8]  Louis A. Romero,et al.  Robust two-dimensional weighted and unweighted phase unwrapping that uses fast transforms and iterative methods , 1994 .

[9]  Quing Zhu,et al.  Quantifying Doppler angle and mapping flow velocity by a combination of Doppler-shift and Doppler-bandwidth measurements in optical Doppler tomography. , 2003, Applied optics.

[10]  R. Zawadzki,et al.  Real-time assessment of retinal blood flow with ultrafast acquisition by color Doppler Fourier domain optical coherence tomography. , 2003, Optics express.

[11]  Theo Lasser,et al.  Vectorial reconstruction of retinal blood flow in three dimensions measured with high resolution resonant Doppler Fourier domain optical coherence tomography. , 2007, Journal of biomedical optics.

[12]  Joseph A Izatt,et al.  In vivo total retinal blood flow measurement by Fourier domain Doppler optical coherence tomography. , 2007, Journal of biomedical optics.

[13]  B L Petrig,et al.  Blood velocity and volumetric flow rate in human retinal vessels. , 1985, Investigative ophthalmology & visual science.