STUDIES ON 5-HYDROXYTRYPTAMINE STORES IN PINEAL GLAND OF RAT.

Summary— The pineal gland of the rat has been studied using a highly specific and sensitive fluorescence microscopical method for the cellular localization of certain biogenic monoamines, in combination with fluorimetric determinations of 5-hydroxytryptamine (5-HT) levels under normal and experimental conditions. Exceptionally large amounts of 5-HT have been found in the gland. This amine is located partly in the parenchymal cells and partly in the pineal sympathetic nerves. There is strong evidence to support the view that the sympathetic nerves are noradrenergic, but take up 5-HT from the parenchymal cells into their terminal portions, which run within the gland. A unique feature thus exists in the sympathetic nerves of the rat's pineal in that they contain two different monoamines. The experiments have shown that half of the pineal 5-HT is located in the sympathetic nerves, and that the rest is stored in the parenchyma. A small portion of this parenchymal pool is dependent on an intact innervation and is lost on denervation. After the injection of noradrenaline, the fluorescence of the pineal nerves changes to show the characteristics of a catecholamine. Half of the pineal 5-HT is then depleted, showing that the neuronal 5-HT is replaced by the administered noradrenaline. No change can be registered in pineal 5-HT levels 22 hours after the administration of α-methyl-m-tyrosine. However, metaraminol causes a pronounced depletion of pineal 5-HT. A reaccumulation of the 5-HT fluorescence in the pineal nerves can be brought about by the injection of nialamide to reserpinized animals, as it occurs in the cerebral nerves containing 5-HT.

[1]  Å. Bertler,et al.  THE DIRECT DEMONSTRATION OF A BARRIER MECHANISM IN THE BRAIN CAPILLARIES. , 2009, Acta pharmacologica et toxicologica.

[2]  B. Brodie,et al.  Occurence of serotonin and catecholamines in brain and peripheral organs of various vertebrate classes , 1963 .

[3]  B. Falck,et al.  Cellular localization of brain monoamines. , 1962, Acta physiologica Scandinavica. Supplementum.

[4]  C. Owman,et al.  THE LOCALIZATION OF ADRENALINE IN ADRENERGIC NERVES IN THE FROG , 1963 .

[5]  B. Werdinius.  Effect of temperature on the action of reserpine. , 2009, Acta pharmacologica et toxicologica.

[6]  W. B. Quay,et al.  Experimental Modification of the Rat Pineal's Content of Serotonin and Related Indole Amines , 1962, Physiological Zoology.

[7]  A. Carlsson,et al.  ROLE OF BRAIN SEROTONIN IN RESERPINE ACTION , 1957, Annals of the New York Academy of Sciences.

[8]  W. B. Quay.  Circadian and Estrous Rhythms in Pineal Melatonin and 5-Hydroxy Indole-3-Acetic Acid.∗ , 1964, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[9]  K. Fuxe,et al.  CELLULAR LOCALIZATION OF MONOAMINES IN THE SPINAL CORD. , 1964, Acta physiologica Scandinavica.

[10]  L. M. Zieher,et al.  THE 5-HYDROXYTRYPTAMINE CONTENT AND SYNTHESIS OF NORMAL AND DENERVATED PINEAL GLAND. , 1963, Life sciences.

[11]  L. S. Phillips,et al.  Melatonin Synthesis in thePineal Gland: Control by Light , 1963, Science.

[12]  R. Wurtman,et al.  Melatonin, a Pineal Substance: Effect on the Rat Ovary , 1963, Science.

[13]  A. Carlsson,et al.  In‐Vivo Decarboxylation of α‐Methyl DOPA and α‐Methyl Metatyrosine , 1962 .

[14]  J. Axelrod,et al.  Intracellular Localization of Catecholamines in Tissues of the Rat , 1962, Nature.

[15]  C. Owman,et al.  EFFECT OF DENERVATION AND ENZYME INHIBITION ON DOPA DECARBOXYLASE AND MONOAMINE OXIDASE ACTIVITIES OF RAT PINEAL GLAND * , 1965, Journal of neurochemistry.

[16]  E. Robertis,et al.  Action of reserpine, iproniazid and pyrogallol on nerve endings of the pineal gland , 1963 .

[17]  B. Brodie,et al.  Identification and assay of serotonin in brain. , 1956, The Journal of pharmacology and experimental therapeutics.

[18]  R. Wurtman,et al.  Melatonin Synthesis in the Pineal Gland: Effect of Light Mediated by the Sympathetic Nervous System , 1964, Science.

[19]  Å. Bertler.  Effect of Reserpine on the Storage of Catechol Amines in Brain and Other Tissues , 1961 .

[20]  H. Corrodi,et al.  Fluoreszenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 1. Identifizierung der fluoreszierenden Produkte aus Modellversuchen mit 6,7‐Dimethoxyisochinolinderivaten und Formaldehyd , 1963 .

[21]  C. Owman.  SYMPATHETIC NERVES PROBABLY STORING TWO TYPES OF MONOAMINES IN THE RAT PINEAL GLAND. , 1964, International journal of neuropharmacology.

[22]  J. A. Kappers,et al.  Demonstration of some compounds present in the pineal organ of the albino rat by hystochemical methods and paper chromatography. , 1961, Acta anatomica.

[23]  N. J. Giarman,et al.  Presence of biogenic amines in the bovine pineal body , 1959 .

[24]  W. B. Quay,et al.  CIRCADIAN RHYTHM IN RAT PINEAL SEROTONIN AND ITS MODIFICATIONS BY ESTROUS CYCLE AND PHOTOPERIOD. , 1963, General and comparative endocrinology.

[25]  H. Weissbach,et al.  Biosynthesis of melatonin: enzymic conversion of serotonin to N-acetylserotonin. , 1960, Biochimica et biophysica acta.

[26]  H. Weissbach,et al.  Enzymatic O-Methylation of N-Acetylserotonin to Melatonin , 1960, Science.

[27]  J. V. Euw,et al.  Fluoreszenzenzmethoden zur histochemischen Sichtbarmachung von Monoaminen. 2.. Identifizierung des fluoreszierenden Produktes aus Dopamin und Formaldehyd , 1964 .

[28]  J. Harvey,et al.  A demonstration of a fall in brain serotonin following central nervous system lesions in the rat. , 1962, Biochemical Pharmacology.

[29]  W. B. Quay.  Differential extractions for the spectrophotofluorometric measurement of diverse 5-hydroxy- and 5-methoxyindoles. , 1963, Analytical biochemistry.

[30]  C. Owman,et al.  A DETAILED METHODOLOGICAL DESCRIPTION OF THE FLUORESCENCE METHOD FOR THE CELLULAR DEMONSTRATION OF BIOGENIC MONOAMINES , 1965 .

[31]  G. Thieme,et al.  FLUORESCENCE OF CATECHOL AMINES AND RELATED COMPOUNDS CONDENSED WITH FORMALDEHYDE , 1962 .