Nebulin regulates actin filament lengths by a stabilization mechanism

The nebulin molecular ruler hypothesis is challenged as a truncated form of nebulin can stabilize actin filaments that are longer than the mini-nebulin itself.

[1]  C. T. Pappas,et al.  Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle , 2010, Journal of Cell Science.

[2]  M. Bang,et al.  Nebulin plays a direct role in promoting strong actin‐myosin interactions , 2009, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[3]  C. Ottenheijm,et al.  Nebulin Alters Cross-bridge Cycling Kinetics and Increases Thin Filament Activation , 2009, The Journal of Biological Chemistry.

[4]  C. Ottenheijm,et al.  Thin filament length dysregulation contributes to muscle weakness in nemaline myopathy patients with nebulin deficiency. , 2009, Human molecular genetics.

[5]  Ju Chen,et al.  Reduced thin filament length in nebulin-knockout skeletal muscle alters isometric contractile properties. , 2009, American journal of physiology. Cell physiology.

[6]  Roberta B. Nowak,et al.  A nebulin ruler does not dictate thin filament lengths. , 2009, Biophysical journal.

[7]  V. Fowler,et al.  Thin filament length regulation in striated muscle sarcomeres: pointed-end dynamics go beyond a nebulin ruler. , 2008, Seminars in cell & developmental biology.

[8]  C. Gregorio,et al.  A myopathy-linked desmin mutation perturbs striated muscle actin filament architecture. , 2008, Molecular biology of the cell.

[9]  C. Ottenheijm,et al.  Sarcoplasmic reticulum calcium uptake and speed of relaxation are depressed in nebulin‐free skeletal muscle , 2008, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[10]  J. Cooper,et al.  Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc. , 2008, Molecular biology of the cell.

[11]  J. Sanger,et al.  Tropomyosin expression and dynamics in developing avian embryonic muscles. , 2008, Cell motility and the cytoskeleton.

[12]  J. Sanger,et al.  Ectopic expression and dynamics of TPM1α and TPM1κ in myofibrils of avian myotubes , 2007 .

[13]  Xiaodi A Deng,et al.  Targeting of nebulin fragments to the cardiac sarcomere. , 2007, Experimental cell research.

[14]  K. Pelin,et al.  Identification of 45 novel mutations in the nebulin gene associated with autosomal recessive nemaline myopathy , 2006, Human mutation.

[15]  Yiming Wu,et al.  Nebulin regulates thin filament length, contractility, and Z‐disk structure in vivo , 2006, The EMBO journal.

[16]  Richard L. Lieber,et al.  Nebulin-deficient mice exhibit shorter thin filament lengths and reduced contractile function in skeletal muscle , 2006, The Journal of cell biology.

[17]  R. Horowits Nebulin regulation of actin filament lengths: new angles. , 2006, Trends in cell biology.

[18]  C. Gregorio,et al.  Nebulin regulates the assembly and lengths of the thin filaments in striated muscle , 2005, The Journal of cell biology.

[19]  J. Sanger,et al.  Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis. , 2005, Cell motility and the cytoskeleton.

[20]  Qiang Zhou,et al.  Dynamics of Z-band based proteins in developing skeletal muscle cells. , 2005, Cell motility and the cytoskeleton.

[21]  Xiaobo Zhou,et al.  Gene Clustering Based on Clusterwide Mutual Information , 2004, J. Comput. Biol..

[22]  C. Gregorio,et al.  The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes , 2003, The Journal of cell biology.

[23]  C. Gregorio,et al.  Nebulin: the nebulous, multifunctional giant of striated muscle. , 2003, Trends in cardiovascular medicine.

[24]  Christian C Witt,et al.  The complete mouse nebulin gene sequence and the identification of cardiac nebulin. , 2003, Journal of molecular biology.

[25]  J. Cooper Actin Dynamics: Tropomyosin Provides Stability , 2002, Current Biology.

[26]  S. Ono,et al.  Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics , 2002, The Journal of cell biology.

[27]  A. Beggs,et al.  Clinical and genetic heterogeneity in nemaline myopathy--a disease of skeletal muscle thin filaments. , 2001, Trends in molecular medicine.

[28]  Velia M. Fowler,et al.  Actin dynamics at pointed ends regulates thin filament length in striated muscle , 2001, Nature Cell Biology.

[29]  M. Bang,et al.  Myopalladin, a Novel 145-Kilodalton Sarcomeric Protein with Multiple Roles in Z-Disc and I-Band Protein Assemblies , 2001, The Journal of cell biology.

[30]  D. Root,et al.  High-affinity actin-binding nebulin fragments influence the actoS1 complex. , 2001, Biochemistry.

[31]  C. Gregorio,et al.  The N-terminal End of Nebulin Interacts with Tropomodulin at the Pointed Ends of the Thin Filaments* , 2001, The Journal of Biological Chemistry.

[32]  M. Bang,et al.  Distinct Families of Z-Line Targeting Modules in the Cooh-Terminal Region of Nebulin , 2000, The Journal of cell biology.

[33]  V. Fowler,et al.  Tropomodulin Increases the Critical Concentration of Barbed End-capped Actin Filaments by Converting ADP·Pi-actin to ADP-actin at All Pointed Filament Ends* , 1999, The Journal of Biological Chemistry.

[34]  Y. Shimada,et al.  Relation of nebulin and connectin (titin) to dynamics of actin in nascent myofibrils of cultured skeletal muscle cells. , 1999, Experimental cell research.

[35]  Nigel,et al.  Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[36]  D. Helfman,et al.  Nonmuscle tropomyosin-4 requires coexpression with other low molecular weight isoforms for binding to thin filaments in cardiomyocytes. , 1999, Journal of cell science.

[37]  L. Kedes,et al.  Altered expression of tropomodulin in cardiomyocytes disrupts the sarcomeric structure of myofibrils. , 1998, Circulation research.

[38]  G. Butler-Browne,et al.  Desmin Is Essential for the Tensile Strength and Integrity of Myofibrils but Not for Myogenic Commitment, Differentiation, and Fusion of Skeletal Muscle , 1997, The Journal of cell biology.

[39]  Y. Capetanaki,et al.  Disruption of muscle architecture and myocardial degeneration in mice lacking desmin , 1996, The Journal of cell biology.

[40]  H. Stedman,et al.  Human Skeletal Muscle Nebulin Sequence Encodes a Blueprint for Thin Filament Architecture , 1996, The Journal of Biological Chemistry.

[41]  S. Lehrer,et al.  Phalloidin unzips nebulin from thin filaments in skeletal myofibrils. , 1995, Journal of cell science.

[42]  C. Gregorio,et al.  Requirement of pointed-end capping by tropomodulin to maintain actin filament length in embryonic chick cardiac myocytes , 1995, Nature.

[43]  C. Gregorio,et al.  Mechanisms of thin filament assembly in embryonic chick cardiac myocytes: tropomodulin requires tropomyosin for assembly , 1995, The Journal of cell biology.

[44]  T. Mikawa,et al.  Antisense suppression of skeletal muscle myosin light chain-1 biosynthesis impairs myofibrillogenesis in cultured myotubes , 1995, Journal of Muscle Research & Cell Motility.

[45]  Jonathan A. Cooper,et al.  Inhibition of CapZ during myofibrillogenesis alters assembly of actin filaments , 1995, The Journal of cell biology.

[46]  V. Fowler,et al.  Tropomodulin caps the pointed ends of actin filaments , 1994, The Journal of cell biology.

[47]  D. Root,et al.  Calmodulin-sensitive interaction of human nebulin fragments with actin and myosin. , 1994, Biochemistry.

[48]  J. Trinick,et al.  Titin and nebulin: protein rulers in muscle? , 1994, Trends in biochemical sciences.

[49]  S J Winder,et al.  Nebulin, a helical actin binding protein. , 1994, The EMBO journal.

[50]  Qi-Quan Huang,et al.  Nebulin is a full-length template of actin filaments in the skeletal muscle sarcomere: an immunoelectron microscopic study of its orientation and span with site-specific monoclonal antibodies , 1993, Journal of Muscle Research & Cell Motility.

[51]  K. Wang,et al.  Nebulin as an actin zipper. A two-module nebulin fragment promotes actin nucleation and stabilizes actin filaments. , 1993, The Journal of biological chemistry.

[52]  K. Wang,et al.  Nebulin as a length regulator of thin filaments of vertebrate skeletal muscles: correlation of thin filament length, nebulin size, and epitope profile , 1991, The Journal of cell biology.

[53]  T. Gibson,et al.  Evidence that nebulin is a protein‐ruler in muscle thin filaments , 1991, FEBS letters.

[54]  Kuan Wang,et al.  Nebulin as a giant actin‐binding template protein in skeletal muscle sarcomere Interaction of actin and cloned human nebulin fragments , 1991, FEBS letters.

[55]  K. Broschat,et al.  Tropomyosin prevents depolymerization of actin filaments from the pointed end. , 1990, The Journal of biological chemistry.

[56]  Jonathan A. Cooper,et al.  Effects of CapZ, an actin capping protein of muscle, on the polymerization of actin. , 1989, Biochemistry.

[57]  S. Brenner,et al.  Inhibition of actin polymerization by latrunculin A , 1987, FEBS letters.

[58]  L. M. Coluccio,et al.  Phalloidin enhances actin assembly by preventing monomer dissociation , 1984, The Journal of cell biology.

[59]  J. Hartwig,et al.  Isolation and some structural and functional properties of macrophage tropomyosin. , 1983, Biochemistry.

[60]  B. L. Granger,et al.  Desmin and vimentin coexist at the periphery of the myofibril Z disc , 1979, Cell.

[61]  U. Lindberg,et al.  Depolymerization of F-actin by deoxyribonuclease I , 1976, Cell.

[62]  J. Aronson SARCOMERE SIZE IN DEVELOPING MUSCLES OF A TARSONEMID MITE , 1961, The Journal of biophysical and biochemical cytology.

[63]  J. Sanger,et al.  Ectopic expression and dynamics of TPM1alpha and TPM1kappa in myofibrils of avian myotubes. , 2007, Cell motility and the cytoskeleton.

[64]  M. Bang,et al.  Molecular dissection of the interaction of desmin with the C-terminal region of nebulin. , 2002, Journal of structural biology.

[65]  M. Beckerle,et al.  Striated muscle cytoarchitecture: an intricate web of form and function. , 2002, Annual review of cell and developmental biology.

[66]  Chen,et al.  Nebulin as an Actin Zipper , 2001 .

[67]  V. Fowler,et al.  Defining actin filament length in striated muscle: rulers and caps or dynamic stability? , 1998, Annual review of cell and developmental biology.

[68]  J. Sanger,et al.  Distribution and orientation of rhodamine-phalloidin bound to thin filaments in skeletal and cardiac myofibrils. , 1997, Cell motility and the cytoskeleton.

[69]  B. Kolmerer,et al.  The complete primary structure of human nebulin and its correlation to muscle structure. , 1995, Journal of molecular biology.

[70]  T. Obinata,et al.  Actin concentration and monomer-polymer ratio in developing chicken skeletal muscle. , 1986, Journal of biochemistry.

[71]  J. Bamburg,et al.  Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF). , 1982, Cell motility.