Study on the adsorption property of lysozyme on weak cation exchanger based on monodisperse poly(glycidymethacrylate-co-ethylenedimethacrylate) beads.

A type of weak cation exchanger was prepared based on poly(glycidylmethacrylate-co-ethylenedimethacrylate). The effects of pH and ionic strength on the adsorption behavior were studied, and the results suggested that the adsorption of lysozyme onto a weak cation exchanger is electrostatic interaction, and that the adsorption behavior is in accordance with the Langmuir adsorption model with a correlation coefficient greater than 0.99. It was also found that increasing ionic strength led to a decrease of the adsorption of lysozyme from 49.50 to 28.09 mg/g. Preliminary chromatographic experiments were conducted to test the separation properties of the weak cation exchanger, and the results demonstrated that the retention time of different proteins could be predicted in order of their isoelectric point.

[1]  张静,et al.  Study on the Adsorption Property of Lysozyme on Weak Cation Exchanger Based on Monodisperse Poly(glycidymethacrylate-co-ethylenedimethacrylate) Beads , 2012 .

[2]  G. Bayramoglu,et al.  Preparation of ion-exchange beads based on poly(methacrylic acid) brush grafted chitosan beads: Isolation of lysozyme from egg white in batch system , 2007 .

[3]  S. Bai,et al.  Fabrication and characterization of rigid magnetic monodisperse microspheres for protein adsorption. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[4]  G. Bayramoglu,et al.  Cytochrome c adsorption on glutamic acid ligand immobilized magnetic poly(methylmethacrylate-co-glycidylmethacrylate) beads , 2007 .

[5]  G. Bayramoglu,et al.  Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin , 2007 .

[6]  Zhiya Ma,et al.  Affinity adsorption of albumin on cibacron blue F3GA-coupled non-porous micrometer-sized magnetic polymer microspheres , 2006 .

[7]  M. N. Gupta,et al.  The affinity concept in bioseparation: evolving paradigms and expanding range of applications. , 2006, Biomolecular engineering.

[8]  G. Bayramoglu,et al.  Invertase reversibly immobilized onto polyethylenimine-grafted poly(GMA–MMA) beads for sucrose hydrolysis , 2006 .

[9]  B. Gong,et al.  Synthesis of non-porous poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads and their application in separation of biopolymers. , 2006, Talanta.

[10]  Yinmao Wei,et al.  Preparation of a monolithic column for weak cation exchange chromatography and its application in the separation of biopolymers. , 2006, Journal of separation science.

[11]  Jianmin Wu,et al.  Chitosan microspheres as immobilized dye affinity support for catalase adsorption. , 2005, International journal of biological macromolecules.

[12]  G. Bayramoglu,et al.  Affinity membrane chromatography: relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[13]  D. Frey,et al.  Charge regulation in protein ion-exchange chromatography: development and experimental evaluation of a theory based on hydrogen ion Donnan equilibrium. , 2004, Journal of chromatography. A.

[14]  B. Gong,et al.  Preparation of hydrophobic interaction chromatographic packings based on monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads and their application. , 2004, Journal of chromatography. A.

[15]  L. F. Bautista,et al.  Application of an effective diffusion model to the adsorption of Aspartame on functionalised divinylbenzene–styrene macroporous resins , 2003 .

[16]  R. Ghosh.  Purification of lysozyme by microporous PVDF membrane-based chromatographic process , 2003 .

[17]  B. Gong,et al.  Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties , 2003, Analytical and bioanalytical chemistry.

[18]  G. Bayramoglu,et al.  Procion Green H-4G immobilized on a new IPN hydrogel membrane composed of poly(2-hydroxyethylmethacrylate)/chitosan: preparation and its application to the adsorption of lysozyme , 2002 .

[19]  J. Fréchet,et al.  High-performance liquid chromatography of complex mixtures using monodisperse dual-chemistry polymer beads prepared by a pore-size-specific functionalization process. A single column combination of hydrophobic interaction and reversed-phase chromatography. , 1994, Analytical chemistry.

[20]  J. Fréchet,et al.  Influence of the seed polymer on the chromatographic properties of size monodisperse polymeric separation media prepared by a multi-step swelling and polymerization method , 1993 .

[21]  F. Švec,et al.  Synthesis and properties of uniform beads based on macroporous copolymer glycidyl methacrylate–ethylene dimethacrylate: A way to improve separation media for HPLC , 1992 .

[22]  F. Regnier,et al.  Mobile phase selection for the high-performance ion-exchange chromatography of proteins. , 1983, Analytical biochemistry.