Effect of dietary supplementation of Lactobacillus acidophilus on blood biochemical profile, antioxidant activity and plasma immunoglobulin level in neonatal Murrah buffalo calves

An experiment was designed to evaluate the effect of dietary supplementation of Lactobacillus acidophilus on blood biochemical profile, antioxidant activity and plasma immunoglobulin level in neonatal Murrah buffalo calves. The 90 day trial was conducted on 24 neonatal Murrah buffalo calves randomly divided into 4 dietary treatments, viz. CON (basal diet alone), T1 (basal diet + L. acidophilus as a fermented milk @ 100 mL/calf/day having 108 CFU/mL), T2 (basal diet + L. acidophilus as a fermented milk @ 200 mL/calf/day having 108 CFU/ml) and T3 (basal diet + L. acidophilus as a fermented milk @ 300 ml/calf/day having 108 CFU/mL). Supplementation of probiotics improved the plasma glucose level in T2 and T3 as compared to CON. Total protein (TP), plasma albumin (A), plasma globulin (G) and A:G ratio did not change with the supplementation of probiotic in calves. Total cholesterol and HDL cholesterol levels in plasma remained same in all the 4 groups. Total antioxidant (TA) activity was higher in T2 and T3 as compared to CON, whereas it was intermediate in T1. Super oxide dismutase (SOD) activity was significantly higher in T1, T2 and T3 groups as compared to CON whereas catalase and glutathione peroxidase (GPx) activity remained same in all groups throughout experimental period. The total plasma immunoglobulin and plasma IgG remained uninfluenced in all the groups. In conclusion, supplementation of L. acidophilus improved energy metabolism and antioxidant capacity in neonatal Murrah buffalo calves.

[1]  M. A. Yoruk,et al.  Effects of Humate and Probiotic on the Number of Escherichia coli, Blood and Antioxidant Parameters in Suckling Period of Calves , 2017 .

[2]  H. Abdi-Benemar,et al.  The Effects of a Medical Plant Mixture and a Probiotic on Performance, Antioxidant Activity and Weaning Age of Newborn Holstein Calves , 2016 .

[3]  Mohammad Noori,et al.  Effect of partial substitution of milk with probiotic yogurt of different pH on performance, body conformation and blood biochemical parameters of Holstein calves , 2016 .

[4]  P. Sharma,et al.  Effect of Probiotic Supplementation on Growth Performance of Pre-Ruminant Buffalo Calves , 2016 .

[5]  N. Dabiri,et al.  Effects of Probiotic and Prebiotic on Average Daily Gain, Fecal Shedding of Escherichia Coli, and Immune System Status in Newborn Female Calves , 2012, Asian-Australasian journal of animal sciences.

[6]  Razia Khan,et al.  SPECTROPHOTOMETRIC METHOD , 2012 .

[7]  F. Masucci,et al.  Performance and immune response of buffalo calves supplemented with probiotic , 2011 .

[8]  Yun-Jaie Choi,et al.  Effect of Feeding Direct-fed Microbial as an Alternative to Antibiotics for the Prophylaxis of Calf Diarrhea in Holstein Calves , 2011 .

[9]  H. Lee,et al.  Lactobacillus plantarum lipoteichoic acid down-regulated Shigella flexneri peptidoglycan-induced inflammation. , 2011, Molecular immunology.

[10]  D. Haines,et al.  Effect of feeding Bovamine® probiotic on passive transfer of immunoglobulin G in newborn calves , 2010 .

[11]  L. Soto,et al.  Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder , 2010 .

[12]  M. Y. Al-Saiady.  Effect of probiotic bacteria on immunoglobulin G concentration and other blood components of newborn calves. , 2010 .

[13]  D. Harmon,et al.  Addition of a Bacillus based probiotic to the diet of preruminant calves: influence on growth, health, and blood parameters. , 2010 .

[14]  D. Kamra,et al.  Effect of direct fed microbials on nutrient utilization, rumen fermentation, immune and growth response in crossbred cattle calves , 2008 .

[15]  R..  Probiotics in man and animals , 2008 .

[16]  H. Rahmani,et al.  Effect of probiotic and milk feeding frequency on performance of dairy Holstein calves. , 2006 .

[17]  D. Kamra,et al.  Effect of Lactic Acid Producing Bacteria on the Performance of Male Crossbred Calves Fed Roughage Based Diet , 2005 .

[18]  S. Cutting,et al.  The use of bacterial spore formers as probiotics. , 2005, FEMS microbiology reviews.

[19]  J. Santos,et al.  Effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal Escherichia coli. , 2005, Reproduction, nutrition, development.

[20]  C. Krehbiel,et al.  Bacterial direct-fed microbials in ruminant diets: Performance response and mode of action , 2003 .

[21]  H. Hammon,et al.  Feed intake patterns, growth performance, and metabolic and endocrine traits in calves fed unlimited amounts of colostrum and milk by automate, starting in the neonatal period. , 2002, Journal of dairy science.

[22]  H. Gill,et al.  Immune protection mediated by the probiotic Lactobacillus rhamnosus HN001 (DR20) against Escherichia coli O157:H7 infection in mice. , 2002, FEMS immunology and medical microbiology.

[23]  J. Evermann,et al.  Biosecurity for neonatal gastrointestinal diseases , 2002, Veterinary Clinics of North America: Food Animal Practice.

[24]  P. Gopal,et al.  Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. , 2001, The American journal of clinical nutrition.

[25]  J. Lumsden.  Clinical Biochemistry of Domestic Animals. , 1998 .

[26]  B. Halliwell,et al.  Antioxidants in human health and disease. , 1996, Annual review of nutrition.

[27]  M. Y. Al-Saiady,et al.  Evaluation of diet containing Lactobacilli on performance, Fecal Coliform, and Lactobacilli of young dairy calves , 1996 .

[28]  A. Feyerherm,et al.  Plasma proteins and a probiotic as ingredients in milk replacer. , 1995, Journal of dairy science.

[29]  S. Marklund,et al.  10-fold increase in human plasma extracellular superoxide dismutase content caused by a mutation in heparin-binding domain. , 1994, The Journal of biological chemistry.

[30]  T. Besser,et al.  The importance of colostrum to the health of the neonatal calf. , 1994, The Veterinary clinics of North America. Food animal practice.

[31]  R. Fuller,et al.  Probiotics in man and animals. , 1989, The Journal of applied bacteriology.

[32]  L. Borg,et al.  A spectrophotometric method for determination of catalase activity in small tissue samples. , 1988, Analytical biochemistry.

[33]  A. Tappel,et al.  Donor substrate specificity and thiol reduction of glutathione disulfide peroxidase. , 1979, The Journal of biological chemistry.

[34]  I. Selman,et al.  A turbidity test for the estimation of immune globulin levels in neonatal calf serum. , 1970, Clinica chimica acta; international journal of clinical chemistry.