Use of the Vδ1 Variable Region in the Functional T‐Cell Receptor α Chain of a WT31+ Cytotoxic T Lymphocyte Clone which Specifically Recognizes HLA‐A2 Molecule

We report here the molecular characterization of the T‐cell receptor (TCR) expressed by a human HLA‐A2 specific cytotoxic T‐cell clone named CTL 49. Flow cytometry analysis with a panel of and‐TCR antibodies revealed an OKT3+, WT31+, A13+, BB3‐, TCRδri‐, δTCS1‐, TCRγ/δ1‐, OKT4‐, and OKT8+ phenotype, suggesting that, in CTL 49, the Vδ1 ‐encoded A13 epitope could be included in its α/β TCR. Northern blot analysis confirmed the presence of Cα, Cβ and Vδ1 specific transcripts while no hybridization signal was detected by a Cδ specific probe. Polymerase chain reaction (PCR) amplification of the first strand cDNA from CTL 49 with TCR‐specfic primers and sequence analysis revealed that Vδ1 region is productively rearranged Jα and to Cα regions. This α chain pairs with a β chain composed of Vβ13.2/Dβ/Jβ2.3/Cβ2 leading to the expression of a functional TCR complex. These results, in addition to providing further evidence for the sharing of Vδ1 by α/β and γ/δ TCR, indicate that an α/β T‐cell receptor which includes the Vδ variable region can be involved in alloreactive recognition.

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