Integrated Multilayer Flow System on a Microchip

We utilized microchip technology and found that the multilayer flow of liquids can be formed in microchannels. Liquid/liquid interfaces were formed parallel to the side wall of the microchannels, because the surface tension and friction force are stronger than the force of gravity. A water/ethylacetate/water interface was formed in a 70-microm-wide and 30-microm-deep channel. The interface was observed to be quite stable and to be maintained for a distance of more than 18 cm. As an example of a multilayer flow application, we demonstrated the liquid/liquid extraction of Co-dimethylaminophenol complex in a microchannel. The solvent-extraction process of the complex into m-xylene in the multilayer flow was found to reach equilibrium in 4 s, while it took 60 s in a simple two-phase extraction.

[1]  T Kitamori,et al.  Non-contact photothermal control of enzyme reactions on a microchip by using a compact diode laser. , 2000, Journal of chromatography. A.

[2]  Takehiko Kitamori,et al.  Photothermal microscopy with excitation and probe beams coaxial under the microscope and its application to microparticle analysis , 1993 .

[3]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[4]  P. Yager,et al.  Diffusion-based extraction in a microfabricated device , 1997 .

[5]  D. Figeys,et al.  Lab-on-a-chip: a revolution in biological and medical sciences , 2000, Analytical chemistry.

[6]  Takehiko Kitamori,et al.  Integrated FIA for the Determination of Ascorbic Acid and Dehydroascorbic Acid in a Microfabricated Glass-Channel by Thermal-Lens Microscopy , 2000 .

[7]  H. Watarai,et al.  Direct Spectrophotometric Measurement of Demetalation Kinetics of 5,10,15,20-Tetraphenylporphyrinatozinc(II) at the Liquid−Liquid Interface by a Centrifugal Liquid Membrane Method , 1998 .

[8]  M. Tokeshi,et al.  Integration of a microextraction system on a glass chip: ion-pair solvent extraction of Fe(II) with 4,7-diphenyl-1,10-phenanthrolinedisulfonic acid and tri-n-octylmethylammonium chloride , 2000, Analytical chemistry.

[9]  B. Finlayson,et al.  Quantitative analysis of molecular interaction in a microfluidic channel: the T-sensor. , 1999, Analytical chemistry.

[10]  T Kitamori,et al.  Integration of an immunosorbent assay system: analysis of secretory human immunoglobulin A on polystyrene beads in a microchip. , 2000, Analytical chemistry.

[11]  T Kitamori,et al.  Integration of a microextraction system solvent extraction of a Co-2-nitroso-5-dimethylaminophenol complex on a microchip. , 2000, Journal of chromatography. A.

[12]  Takehiko Kitamori,et al.  Integration of Flow Injection Analysis and Zeptomole-Level Detection of the Fe(II)-o-Phenanthroline Complex , 1999 .