论文信息 - A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.

A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.

Abstract A preparative procedure for obtaining highly purified plasmid DNA from bacterial cells is described. The method is adapted from our earlier procedure, which gave partially purified plasmid in a form suitable for rapid screening of a large number of samples. In the present method, all detectable RNA, chromosomal DNA, and protein are removed without the use of enzymes, phenol extraction, dialysis, or equilibrium centrifugation. Binding of plasmid DNA to glass powder in the presence of 6 m sodium perchlorate is used for the final purification step.

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