Development of an inductively coupled MR coil system for imaging and spectroscopic analysis of an implantable bioartificial construct at 11.1 T

Developing a method to noninvasively monitor tissue‐engineered constructs is critical for the optimization of construct design and for assessing therapeutic efficacy. For this purpose, NMR is a powerful technique that can be used to obtain both images and spectroscopic data. But the inherent sensitivity of NMR limits the observation of a bioartificial construct with current NMR surface coil technology. In this study, we address this limitation through the development of an inductively coupled, implantable coil system, demonstrate its use at high field (11.1 T), and investigate the use of this coil system for monitoring a bioartificial construct in vitro and in vivo. The results establish that large gains in signal to noise can be obtained with this coil system over that obtainable with a surface coil. This coil system provides a means to quantitatively analyze the structure and function of implanted bioartificial organs. Magn Reson Med 63:998–1006, 2010. © 2010 Wiley‐Liss, Inc.

[1]  In situ magnetic resonance microscopy. , 1987, Investigative radiology.

[2]  C Gabriel,et al.  The dielectric properties of biological tissues: I. Literature survey. , 1996, Physics in medicine and biology.

[3]  E. Purcell,et al.  Relaxation Effects in Nuclear Magnetic Resonance Absorption , 1948 .

[4]  G. Randy Duensing,et al.  Common mode signal rejection methods for MRI: Reduction of cable shield currents for high static magnetic field systems , 2003 .

[5]  G. Skjåk‐Braek,et al.  SUCCESSFUL REVERSAL OF SPONTANEOUS DIABETES IN DOGS BY INTRAPERITONEAL MICROENCAPSULATED ISLETS , 1992, Transplantation.

[6]  J. S. Leigh,et al.  Wireless implanted magnetic resonance probes for in vivo NMR , 1986 .

[7]  N. Simpson,et al.  Effects of growth regulation on conditionally-transformed alginate-entrapped insulin secreting cell lines in vitro. , 2005, Biomaterials.

[8]  R. W. Lau,et al.  The dielectric properties of biological tissues: II. Measurements in the frequency range 10 Hz to 20 GHz. , 1996, Physics in medicine and biology.

[9]  L. Whitehead,et al.  Split-ring resonator for use in magnetic resonance from 200-2000 MHz , 1981 .

[10]  A. Sambanis,et al.  Noninvasive measurement of viable cell number in tissue-engineered constructs in vitro, using 1H nuclear magnetic resonance spectroscopy. , 2005, Tissue engineering.

[11]  Lance L. Arnder,et al.  Signal‐to‐noise ratio comparison between surface coils and implanted coils , 1996, Magnetic resonance in medicine.

[12]  P. Lauterbur,et al.  The sensitivity of the zeugmatographic experiment involving human samples , 1979 .

[13]  F. E. Terman,et al.  Radio Engineers Handbook , 1943 .

[14]  T. Mareci,et al.  A comparison of an inductively coupled implanted coil with optimized surface coils for in vivo NMR imaging of the spinal cord , 1993, Magnetic resonance in medicine.

[15]  A. Sambanis,et al.  In Vivo Noninvasive Monitoring of a Tissue Engineered Construct Using 1H NMR Spectroscopy , 2005, Cell transplantation.

[16]  P. Narayana,et al.  In vivo relaxation times of gray matter and white matter in spinal cord. , 1999, Magnetic resonance imaging.

[17]  Athanassios Sambanis,et al.  Noninvasive Monitoring of a Retrievable Bioartificial Pancreas in Vivo , 2002, Annals of the New York Academy of Sciences.

[18]  A Jesmanowicz,et al.  Counter rotating current local coils for high‐resolution magnetic resonance imaging , 1986, Magnetic resonance in medicine.

[19]  A. Sambanis,et al.  Noninvasive Monitoring of Tissue-Engineered Constructs by Nuclear Magnetic Resonance Methodologies , 1998 .

[20]  An Artificial Endocrine Pancreas Containing Cultured Islets of Langerhans , 2008 .

[21]  T. Mareci,et al.  In vivo 1H magnetic resonance imaging and spectroscopy of the rat spinal cord using an inductively‐coupled chronically implanted RF coil , 2001, Magnetic resonance in medicine.

[22]  P A Narayana,et al.  In vivo echo-planar imaging of rat spinal cord. , 1998, Magnetic resonance imaging.

[23]  R. Henkelman.  Measurement of signal intensities in the presence of noise in MR images. , 1985, Medical physics.

[24]  A. Sun,et al.  Microencapsulation of living cells — A long-term delivery system☆ , 1985 .

[25]  Wei Chen,et al.  Measurement of arterial input function of 17O water tracer in rat carotid artery by using a region-defined (REDE) implanted vascular RF coil , 2003, Magnetic Resonance Materials in Physics, Biology and Medicine.

[26]  R. W. Lau,et al.  The dielectric properties of biological tissues: III. Parametric models for the dielectric spectrum of tissues. , 1996, Physics in medicine and biology.

[27]  M. Mateus,et al.  An overview on the development of a bio‐artificial pancreas as a treatment of insulin‐dependent diabetes mellitus , 2006, Medicinal research reviews.

[28]  A. Sambanis,et al.  Non‐Invasive Monitoring of a Bioartificial Pancreas in Vitro and in Vivo , 2001, Annals of the New York Academy of Sciences.

[29]  David I. Hoult,et al.  Use of mutually inductive coupling in probe design , 2002 .

[30]  Mehmet Bilgen,et al.  Inductively-overcoupled coil design for high resolution magnetic resonance imaging , 2006, Biomedical engineering online.

[31]  Terence W Nixon,et al.  High magnetic field water and metabolite proton T1 and T2 relaxation in rat brain in vivo , 2006, Magnetic resonance in medicine.