The effect of mesoporous bioactive glass on the physiochemical, biological and drug-release properties of poly(DL-lactide-co-glycolide) films.

Poly(lactide-co-glycolide) (PLGA) has been widely used for bone tissue regeneration. However, it lacks hydrophilicity, bioactivity and sufficient mechanical strength and its acidic degradation by-products can lead to pH decrease in the vicinity of the implants. Mesoporous bioactive glass (MBG) with highly ordered structure (pore size 2-50nm) possesses higher bioactivity than non-mesoporous bioactive glass (BG). The aim of this study is to investigate the effect of MBG on the mechanical strength, in vitro degradation, bioactivity, cellular response and drug release of PLGA films and optimize their physicochemical, biological and drug-delivery properties for bone tissue engineering application. The surface and inner microstructure, mechanical strength and surface hydrophilicity of MBG/PLGA and BG/PLGA films were tested. Results indicated that MBG or BG was uniformly dispersed in the PLGA films. The incorporation of MBG into PLGA films significantly improved their tensile strength, modulus and surface hydrophilicity. MBG/PLGA resulted in an enhanced mechanical strength, in vitro degradation (water absorbance, weight loss and ions release), apatite-formation ability and pH stability in simulated body fluids (SBF), compared to BG/PLGA. MBG/PLGA and BG/PLGA films enhanced human osteoblastic-like cells (HOBs) attachment, spreading and proliferation compared to PLGA. HOBs differentiation was significantly upregulated when cells were cultured on 30 MBG/PLGA for 14 days, compared to 30 BG/PLGA. MBG/PLGA enhanced the accumulative release of dexamethazone (DEX) at early stages (0-200h) compared to BG/PLGA, however, after 200h, DEX-release rates for MBG/PLGA was slower than that of BG/PLGA. The contents of MBG in PLGA films can control the amount of DEX released. Taken together, MBG/PLGA films possessed excellent physicochemical, biological and drug-release properties, indicating their potential application for bone tissue engineering by designing 3D scaffolds according to their corresponding compositions.

[1]  Jia-cong Shen,et al.  Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. , 2005, Biomaterials.

[2]  Chengtie Wu,et al.  Degradation, bioactivity, and cytocompatibility of diopside, akermanite, and bredigite ceramics. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[3]  Sheryl E. Philip,et al.  Comparison of nanoscale and microscale bioactive glass on the properties of P(3HB)/Bioglass composites. , 2008, Biomaterials.

[4]  Chengtie Wu,et al.  In vitro bioactivity of akermanite ceramics. , 2006, Journal of biomedical materials research. Part A.

[5]  J. Blaker,et al.  Three-dimensional culture of annulus fibrosus cells within PDLLA/Bioglass composite foam scaffolds: assessment of cell attachment, proliferation and extracellular matrix production. , 2007, Biomaterials.

[6]  D. Zaffe,et al.  Polylactide/polyglycolide copolymer in bone defect healing in humans. , 2008, Biomaterials.

[7]  Xuesi Chen,et al.  In vivo mineralization and osteogenesis of nanocomposite scaffold of poly(lactide-co-glycolide) and hydroxyapatite surface-grafted with poly(L-lactide). , 2009, Biomaterials.

[8]  Jianlin Shi,et al.  A mesoporous bioactive glass/polycaprolactone composite scaffold and its bioactivity behavior. , 2008, Journal of biomedical materials research. Part A.

[9]  Chengtie Wu,et al.  Incorporation of titanium into calcium silicate improved their chemical stability and biological properties. , 2008, Journal of biomedical materials research. Part A.

[10]  Jiang Chang,et al.  pH-compensation effect of bioactive inorganic fillers on the degradation of PLGA , 2005 .

[11]  J. Tanaka,et al.  Preparation and mechanical properties of calcium phosphate/copoly-L-lactide composites , 1997, Journal of materials science. Materials in medicine.

[12]  A. Boccaccini,et al.  Fabrication and characterization of biodegradable poly(3-hydroxybutyrate) composite containing bioglass. , 2007, Biomacromolecules.

[13]  Chengtie Wu,et al.  Improvement of mechanical and biological properties of porous CaSiO3 scaffolds by poly(D,L-lactic acid) modification. , 2008, Acta biomaterialia.

[14]  Jiang Chang,et al.  Preparation and characterization of bioactive and biodegradable Wollastonite/poly(D,L-lactic acid) composite scaffolds , 2004, Journal of materials science. Materials in medicine.

[15]  L L Hench,et al.  Surface-active biomaterials. , 1984, Science.

[16]  Aldo R Boccaccini,et al.  PDLLA/Bioglass composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment. , 2004, Biomaterials.

[17]  L L Hench,et al.  Biomaterials: a forecast for the future. , 1998, Biomaterials.

[18]  Hala Zreiqat,et al.  The effect of strontium incorporation into CaSiO3 ceramics on their physical and biological properties. , 2007, Biomaterials.

[19]  Chengtie Wu,et al.  Plasma-sprayed CaTiSiO5 ceramic coating on Ti-6Al-4V with excellent bonding strength, stability and cellular bioactivity , 2009, Journal of The Royal Society Interface.

[20]  常江.  Well-ordered mesoporous bioactive glasses(MBG):A promising bioactive drug delivery system , 2006 .

[21]  E. Wintermantel,et al.  pH-stabilization of predegraded PDLLA by an admixture of water-soluble sodiumhydrogenphosphate--results of an in vitro- and in vivo-study. , 2002, Biomaterials.

[22]  A. Simmons,et al.  Biostability and biological performance of a PDMS-based polyurethane for controlled drug release. , 2008, Biomaterials.

[23]  M. Watanabe,et al.  Effect of blending calcium compounds on hydrolytic degradation of poly(DL-lactic acid-co-glycolic acid). , 2002, Biomaterials.

[24]  Xufeng Zhou,et al.  Highly ordered mesoporous bioactive glasses with superior in vitro bone-forming bioactivities. , 2004, Angewandte Chemie.

[25]  Chengtie Wu,et al.  The responses of osteoblasts, osteoclasts and endothelial cells to zirconium modified calcium-silicate-based ceramic. , 2008, Biomaterials.

[26]  Drug delivery: the heart of the matter. , 2008, Nature materials.

[27]  M. Cerruti,et al.  Silicate Biomaterials for Orthopaedic and Dental Implants , 2006 .

[28]  Jiang Chang,et al.  Preparation, characterization and in vitro release of gentamicin from PHBV/wollastonite composite microspheres. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[29]  Stefan Kaskel,et al.  Preparation, characterization and in vitro bioactivity of mesoporous bioactive glasses (MBGs) scaffolds for bone tissue engineering , 2008 .

[30]  W. Stark,et al.  Improved degradation and bioactivity of amorphous aerosol derived tricalcium phosphate nanoparticles in poly(lactide-co-glycolide) , 2006 .

[31]  Zu-wei Ma,et al.  Surface modification and property analysis of biomedical polymers used for tissue engineering. , 2007, Colloids and surfaces. B, Biointerfaces.

[32]  Jiang Chang,et al.  Fabrication and characterization of bioactive wollastonite/PHBV composite scaffolds. , 2004, Biomaterials.

[33]  J. Feijen,et al.  Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. , 1985, Biomaterials.

[34]  L L Hench,et al.  An investigation of bioactive glass powders by sol-gel processing. , 1991, Journal of applied biomaterials : an official journal of the Society for Biomaterials.

[35]  Chengtie Wu,et al.  The effect of Zn contents on phase composition, chemical stability and cellular bioactivity in Zn-Ca-Si system ceramics. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.