Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes

The chromosomal location of human constant region light chain immunoglobulin (Ig) genes has been determined by analyzing a group of human fibroblast/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human kappa and lambda constant region genes. Human chromosomes in each cell line were identified by isoenzyme analysis. The DNA from hybrid cells was digested with restriction endonucleases, size fractionated by gel electrophoresis, transferred to nitrocellulose or DBM paper, and hybridized with (32)P-labeled nucleic acid probes. The C(κ) gene was assigned to human chromosome 2 and the C(λ) genes to chromosome 22, based upon analysis of these hybrid cell lines, and these assignments were confirmed by analysis of subclones. A group of previously unassigned loci can be mapped to chromosome 2 by virtue of their close linkage to C(κ). The λ and κ light chain and heavy chain Ig genes have now been assigned to all three human chromosomes that are involved in translocations with chromosome 8 in human B cell neoplasms. These techniques and probes provide a means to study the detailed arrangement of human Ig genes and their pseudogenes.

[1]  T. Waldmann,et al.  Clustered arrangement of immunoglobulin λ constant region genes in man , 1981, Nature.

[2]  S. Povey,et al.  Immunoglobulin heavy chain genes in humans are located on chromosome 14 , 1981 .

[3]  R. Berger,et al.  Cytogenetic studies on African Burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations. , 1981, Cancer genetics and cytogenetics.

[4]  T. Waldmann,et al.  Human immunoglobulin κ light-chain genes are deleted or rearranged in λ-producing B cells , 1981, Nature.

[5]  P. D’Eustachio,et al.  Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains , 1981, The Journal of experimental medicine.

[6]  T. Waldmann,et al.  Clustered arrangement of immunoglobulin lambda constant region genes in man. , 1981, Nature.

[7]  K. Hirschhorn,et al.  Confirmation of the assignment of genes for human immunoglobulin heavy chains to chromosome 14 by analysis of Ig synthesis by man‐mouse hybridomas , 1981, European journal of immunology.

[8]  J. Seidman,et al.  Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments , 1980, Cell.

[9]  H. Orr,et al.  Molecular cloning of a human histocompatibility antigen cDNA fragment. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[10]  S P Kwan,et al.  Immunoglobulin V/J recombination is accompanied by deletion of joining site and variable region segments. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[11]  P. D’Eustachio,et al.  Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains , 1980, The Journal of experimental medicine.

[12]  J. Rowley.  Chromosome abnormalities in human leukemia. , 1980, Annual review of genetics.

[13]  G. Klein,et al.  Nonrandom chromosome changes involving the Ig gene-carrying chromosomes 12 and 6 in pristane-induced mouse plasmacytomas , 1979, Cell.

[14]  Hitoshi Sakano,et al.  Sequences at the somatic recombination sites of immunoglobulin light-chain genes , 1979, Nature.

[15]  H. Koprowski,et al.  Chromosomal location of the genes for human immunoglobulin heavy chains. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[16]  P. D’Eustachio,et al.  Chromosomal assignment of the mouse kappa light chain genes. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[17]  G R Stark,et al.  Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. , 1979, Methods in enzymology.

[18]  T. Shows,et al.  Genetics of the large, external, transformation-sensitive (LETS) protein: assignment of a gene coding for expression of LETS to human chromosome 8. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Susumu Tonegawa,et al.  A Complete Immunoglobulin Gene Is Created by Somatic Recombination , 2004 .

[20]  Susumu Tonegawa,et al.  A complete immunoglobulin gene is created by somatic recombination , 1978, Cell.

[21]  J. Seidman,et al.  High capacity gel preparative electrophoresis for purification of fragments of genomic DNA. , 1978, Analytical biochemistry.

[22]  P Berg,et al.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. , 1977, Journal of molecular biology.

[23]  D. N. Raine,et al.  Handbook of Enzyme Electrophoresis in Human Genetics , 1977 .

[24]  F. Hecht,et al.  Mechanisms of giemsa banding of chromosomes. I. Giemsa-11 banding with azure and eosin. , 1976, Experimental cell research.

[25]  R. Davidson,et al.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol , 1976, Somatic cell genetics.

[26]  G. Klein,et al.  Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas , 1976, International journal of cancer.

[27]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[28]  K. Grzeschik,et al.  Identification of human RNA transcripts among heterogeneous nuclear RNA from man-mouse somatic cell hybrids. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[29]  F. Ruddle,et al.  A REVIEW OF ENZYME POLYMORPHISM, LINKAGE AND ELECTROPHORETIC CONDITIONS FOR MOUSE AND SOMATIC CELL HYBRIDS IN STARCH GELS , 1973, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[30]  M. Seabright.  A rapid banding technique for human chromosomes. , 1971, Lancet.

[31]  B. Ephrussi,et al.  Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J. Littlefield.  The use of drug-resistant markers to study the hybridization of mouse fibroblasts. , 1966, Experimental cell research.

[33]  D. R. Dubbs,et al.  DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE. , 1963, Experimental cell research.