Identification of circulating microRNA signatures for upper tract urothelial carcinoma detection

The involvement of circulating microRNAs (miRNAs) in cancer and their potential as diagnostic and prognostic biomarkers are becoming increasingly known. However, the significance of circulating miRNAs in upper tract urothelial carcinoma (UTUC) has remained to be investigated. The present study performed a genome wide serum miRNA analysis using a deep sequencing platform for initial screening. Subsequently, serum samples of 46 UTUC patients and 30 cancer free individuals with hematuria were subjected to a quantitative reverse transcription polymerase chain reaction analysis. The expression of thirteen miRNAs (miR-664a-3p, miR-423-5p, miR-431-5p, miR-191-5p, miR-92a-3p, miR-22-3p, miR-26a-5p, miR-33b-3p, miR-16-5p, let-7a 5p, let-7b-5p, let-7f-5p and let-7c) was significantly different in serum from UTUC patients compared with that in control samples. Receiver operator characteristic analysis showed that 10 miRNAs (miR-664a-3p, miR-431-5p, miR-423-5p, miR-191-5p, miR-33b-3p, miR-26a-5p, miR-22-3p, miR-16-5p, let-7b-5p and let-7c) had the potential to distinguish individuals with UTUC from the controls (areas under the curve >0.8). The present study provided the first evidence for the potential use of circulating miRNAs as biomarkers for UTUC diagnosis, which remains to be verified by further studies.

[1]  R. Gregory,et al.  MicroRNA biogenesis pathways in cancer , 2015, Nature Reviews Cancer.

[2]  P. Colin,et al.  Prognostic value of microRNA expression pattern in upper tract urothelial carcinoma , 2014, BJU international.

[3]  J. Shao,et al.  Profiling plasma microRNA in nasopharyngeal carcinoma with deep sequencing. , 2014, Clinical chemistry.

[4]  P. Kapur,et al.  Prospective comparison of molecular signatures in urothelial cancer of the bladder and the upper urinary tract--is there evidence for discordant biology? , 2014, The Journal of urology.

[5]  Wei Li,et al.  Identification and Validation of Reference Genes for qPCR Detection of Serum microRNAs in Colorectal Adenocarcinoma Patients , 2013, PloS one.

[6]  J. Lozano,et al.  Using microRNA profiling in urine samples to develop a non‐invasive test for bladder cancer , 2013, International journal of cancer.

[7]  C. Woods,et al.  Plasma microRNA signature as a noninvasive biomarker for acute graft-versus-host disease. , 2013, Blood.

[8]  Wang Jing,et al.  Plasma microRNA profiles for bladder cancer detection. , 2013, Urologic oncology.

[9]  W. Chan,et al.  MicroRNA expression profiling identifies molecular signatures associated with anaplastic large cell lymphoma. , 2013, Blood.

[10]  S. Rai,et al.  A Plasma MicroRNA Panel for Detection of Colorectal Adenomas: A Step Toward More Precise Screening for Colorectal Cancer , 2013, Annals of surgery.

[11]  Jian Gu,et al.  Pathway-based serum microRNA profiling and survival in patients with advanced stage non-small cell lung cancer. , 2013, Cancer research.

[12]  Y. Doki,et al.  Circulating miR-200c Levels Significantly Predict Response to Chemotherapy and Prognosis of Patients Undergoing Neoadjuvant Chemotherapy for Esophageal Cancer , 2013, Annals of Surgical Oncology.

[13]  Xiaoyi Huang,et al.  Extracellular MicroRNAs in Urologic Malignancies: Chances and Challenges , 2013, International journal of molecular sciences.

[14]  Mario Boccadoro,et al.  Biological and Clinical Relevance of miRNA Expression Signatures in Primary Plasma Cell Leukemia , 2013, Clinical Cancer Research.

[15]  Jae Kwan Lee,et al.  Detection of MicroRNA as Novel Biomarkers of Epithelial Ovarian Cancer From the Serum of Ovarian Cancer Patient , 2013, International Journal of Gynecologic Cancer.

[16]  Shelly C. Lu,et al.  MicroRNAs regulate methionine adenosyltransferase 1A expression in hepatocellular carcinoma. , 2013, The Journal of clinical investigation.

[17]  G. Kristiansen,et al.  Evaluation of reference genes for the analysis of serum miRNA in patients with prostate cancer, bladder cancer and renal cell carcinoma , 2012, International journal of urology : official journal of the Japanese Urological Association.

[18]  V. Jung,et al.  Analysis of serum microRNAs (miR-26a-2*, miR-191, miR-337-3p and miR-378) as potential biomarkers in renal cell carcinoma. , 2012, Cancer epidemiology.

[19]  K. Horgan,et al.  Circulating microRNA profiles reflect the presence of breast tumours but not the profiles of microRNAs within the tumours , 2012, Cellular Oncology.

[20]  R. Hautmann,et al.  Radical cystectomy for urothelial carcinoma of the bladder without neoadjuvant or adjuvant therapy: long-term results in 1100 patients. , 2012, European urology.

[21]  M. Tewari,et al.  MicroRNA profiling: approaches and considerations , 2012, Nature Reviews Genetics.

[22]  F. Yu,et al.  Circulating microRNA profiles as potential biomarkers for diagnosis of papillary thyroid carcinoma. , 2012, The Journal of clinical endocrinology and metabolism.

[23]  Christine A Iacobuzio-Donahue,et al.  A new branch on the tree: next-generation sequencing in the study of cancer evolution. , 2012, Seminars in cell & developmental biology.

[24]  K. Zen,et al.  Circulating MicroRNAs: a novel class of biomarkers to diagnose and monitor human cancers , 2012, Medicinal research reviews.

[25]  G. Calin,et al.  MicroRNAs in body fluids—the mix of hormones and biomarkers , 2011, Nature Reviews Clinical Oncology.

[26]  Volker Hovestadt,et al.  Molecular and Cellular Pathobiology MicroRNA Sequence and Expression Analysis in Breast Tumors by Deep Sequencing , 2011 .

[27]  J. Ellinger,et al.  Circulating microRNAs (miRNA) in serum of patients with prostate cancer. , 2011, Urology.

[28]  M. Babjuk,et al.  European guidelines for the diagnosis and management of upper urinary tract urothelial cell carcinomas: 2011 update. , 2011, European urology.

[29]  Xi Chen,et al.  A five-microRNA signature identified from genome-wide serum microRNA expression profiling serves as a fingerprint for gastric cancer diagnosis. , 2011, European journal of cancer.

[30]  Zhongfa Zhang,et al.  Comparative gene expression profiling analysis of urothelial carcinoma of the renal pelvis and bladder , 2010, BMC Medical Genomics.

[31]  Xi Chen,et al.  Expression profile of microRNAs in serum: a fingerprint for esophageal squamous cell carcinoma. , 2010, Clinical chemistry.

[32]  X. Chen,et al.  Serum microRNA profiles serve as novel biomarkers for HBV infection and diagnosis of HBV-positive hepatocarcinoma. , 2010, Cancer research.

[33]  Perry D Moerland,et al.  MiR423-5p As a Circulating Biomarker for Heart Failure , 2010, Circulation research.

[34]  Wei Shi,et al.  Comprehensive MicroRNA Profiling for Head and Neck Squamous Cell Carcinomas , 2010, Clinical Cancer Research.

[35]  C. Croce.  Causes and consequences of microRNA dysregulation in cancer , 2009, Nature Reviews Genetics.

[36]  C. Atreya,et al.  Membrane array–based differential profiling of platelets during storage for 52 miRNAs associated with apoptosis , 2009, Transfusion.

[37]  Y. Lotan,et al.  Outcomes of radical nephroureterectomy: A series from the Upper Tract Urothelial Carcinoma Collaboration , 2009, Cancer.

[38]  R. Gregory,et al.  Many roads to maturity: microRNA biogenesis pathways and their regulation , 2009, Nature Cell Biology.

[39]  X. Chen,et al.  Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases , 2008, Cell Research.

[40]  Daniel B. Martin,et al.  Circulating microRNAs as stable blood-based markers for cancer detection , 2008, Proceedings of the National Academy of Sciences.

[41]  E. Wiemer,et al.  miRNAs and cancer , 2006, Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research.

[42]  O. Cussenot,et al.  Tissue microarray analysis of the prognostic value of E-cadherin, Ki67, p53, p27, survivin and MSH2 expression in upper urinary tract transitional cell carcinoma. , 2005, European urology.

[43]  K. Nakanishi,et al.  Expression of Hypoxia-Inducible Factor-1α Protein Predicts Survival in Patients with Transitional Cell Carcinoma of the Upper Urinary Tract , 2005, Clinical Cancer Research.

[44]  L. Ellison,et al.  Upper tract urothelial neoplasms: incidence and survival during the last 2 decades. , 2000, The Journal of urology.

[45]  K. Nakanishi,et al.  Expression of telomerase mRNA component (hTR) in transitional cell carcinoma of the upper urinary tract , 1999, Cancer.

[46]  T. Carmody,et al.  Prognostic factors, recurrence, and survival in transitional cell carcinoma of the upper urinary tract: a 30-year experience in 252 patients. , 1998, Urology.

[47]  O. Slabý,et al.  Urine microRNAs as potential noninvasive biomarkers in urologic cancers. , 2014, Urologic oncology.

[48]  Xi Chen,et al.  Identification of seven serum microRNAs from a genome‐wide serum microRNA expression profile as potential noninvasive biomarkers for malignant astrocytomas , 2013, International journal of cancer.

[49]  Leslie H. Sobin,et al.  TNM Classification of Malignant Tumours, 7th Edition , 2009 .

[50]  K. Nakanishi,et al.  Expression of hypoxia-inducible factor-1alpha protein predicts survival in patients with transitional cell carcinoma of the upper urinary tract. , 2005, Clinical cancer research : an official journal of the American Association for Cancer Research.

[51]  L. Sobin,et al.  TNM Classification of Malignant Tumours , 1987, UICC International Union Against Cancer.