Structured illumination in compact and field-portable 3D-printed shearing digital holographic microscopy for resolution enhancement.

A compact and field-portable three-dimensional (3D)-printed structured illumination (SI) digital holographic microscope based on shearing geometry is presented. By illuminating the sample using a SI pattern, the lateral resolution in both reconstructed phase and amplitude images can be improved up to twice the resolution provided by conventional illumination. The use of a 3D-printed system and shearing geometry reduces the complexity of the system, while providing high temporal stability. The experimental results for the USAF resolution target show a resolution improvement of a factor of two which corroborates the theoretical prediction. Resolution enhancement in phase imaging is also demonstrated by imaging a biological sample. To the best of our knowledge, this is the first report of a compact and field-portable SI digital holographic system based on shearing geometry.

[1]  Bahram Javidi,et al.  Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd's mirror. , 2012, Optics letters.

[2]  Ehsan A. Akhlaghi,et al.  Super-resolved Mirau digital holography by structured illumination , 2017 .

[3]  Genaro Saavedra,et al.  Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination. , 2014, Optics letters.

[4]  Bahram Javidi,et al.  Sickle cell disease diagnosis based on spatio-temporal cell dynamics analysis using 3D printed shearing digital holographic microscopy. , 2018, Optics express.

[5]  Ana Doblas,et al.  Off-axis digital holographic microscopy: practical design parameters for operating at diffraction limit. , 2014, Applied optics.

[6]  Frank Dubois,et al.  Automated three-dimensional detection and classification of living organisms using digital holographic microscopy with partial spatial coherent source: application to the monitoring of drinking water resources. , 2013, Applied optics.

[7]  Bahram Javidi,et al.  Compact and field-portable 3D printed shearing digital holographic microscope for automated cell identification. , 2017, Applied optics.

[8]  M. Gustafsson Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy , 2000, Journal of microscopy.

[9]  Yonina C. Eldar,et al.  Coherent superresolution imaging via grating-based illumination , 2017 .

[10]  Osamu Matoba,et al.  Multimodal Imaging Based on Digital Holography , 2017, Proceedings of the IEEE.

[11]  A. Doblas,et al.  Diabetes screening by telecentric digital holographic microscopy , 2016, Journal of microscopy.

[12]  Pietro Ferraro,et al.  Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging. , 2003, Applied optics.

[13]  C. Werner,et al.  Satellite radar interferometry: Two-dimensional phase unwrapping , 1988 .

[14]  Joseph Izatt,et al.  Structured illumination quantitative phase microscopy for enhanced resolution amplitude and phase imaging. , 2013, Biomedical optics express.

[15]  A Finizio,et al.  Angular spectrum method with correction of anamorphism for numerical reconstruction of digital holograms on tilted planes. , 2005, Optics express.

[16]  B. Javidi,et al.  Automatic Identification of Malaria-Infected RBC With Digital Holographic Microscopy Using Correlation Algorithms , 2012, IEEE Photonics Journal.

[17]  Bahram Javidi,et al.  Lateral shearing digital holographic imaging of small biological specimens. , 2012, Optics express.

[18]  Bahram Javidi,et al.  Cell morphology-based classification of red blood cells using holographic imaging informatics. , 2016, Biomedical optics express.

[19]  O. Matoba,et al.  Single-shot incoherent digital holography using a dual-focusing lens with diffraction gratings. , 2017, Optics letters.

[20]  Jonghee Yoon,et al.  Holographic deep learning for rapid optical screening of anthrax spores , 2017, Science Advances.

[21]  Joon Y. Choe,et al.  Nonmechanical image rotation with an acousto-optic dove prism. , 1997 .