Highly sensitive dopamine biosensors based on organic electrochemical transistors.

Organic electrochemical transistors (OECTs) based on poly(3,4-ethylenedioxythiophene):poly(styrene sulfonic acid) (PEDOT:PSS) with different gate electrodes, including graphite, Au and Pt electrode, etc., have been used as dopamine sensor for the first time. The sensitivity of the OECT to dopamine depends on its gate electrode and operation voltage. We find that the device with a Pt gate electrode characterized at the gate voltage of 0.6 V shows the highest sensitivity. The detection limit of the device to dopamine is lower than 5 nM, which is one order of magnitude better than a conventional electrochemical measurement with the same Pt electrode. It is expected that OECT is a good candidate for low cost and highly sensitive biosensor for the detection of dopamine.

[1]  T. Ogihara,et al.  Free and total dopamine in human plasma: effects of posture, age and some pathophysiological conditions. , 1995, Hypertension research : official journal of the Japanese Society of Hypertension.

[2]  Zhenan Bao,et al.  Fabrication of low-cost electronic biosensors , 2009 .

[3]  J. A. Jankowski,et al.  Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[4]  N. Lee,et al.  Organic electrochemical transistor based immunosensor for prostate specific antigen (PSA) detection using gold nanoparticles for signal amplification. , 2010, Biosensors & bioelectronics.

[5]  Mark A. Reed,et al.  Label-free immunodetection with CMOS-compatible semiconducting nanowires , 2007, Nature.

[6]  J. Horvitz,et al.  Dopaminergic Mechanisms in Actions and Habits , 2007, The Journal of Neuroscience.

[7]  Xi Chen,et al.  Dopaminergic Signaling and Striatal Neurodegeneration in Huntington's Disease , 2007, The Journal of Neuroscience.

[8]  Huimin Zhang,et al.  Electrocatalytic response of dopamine at a dl-homocysteine self-assembled gold electrode , 2000 .

[9]  George G. Malliaras,et al.  Steady‐State and Transient Behavior of Organic Electrochemical Transistors , 2007 .

[10]  Itamar Willner,et al.  Analysis of dopamine and tyrosinase activity on ion-sensitive field-effect transistor (ISFET) devices. , 2007, Chemistry.

[11]  Debjani Paul,et al.  Label-free sub-picomolar protein detection with field-effect transistors. , 2010, Analytical chemistry.

[12]  George G. Malliaras,et al.  Enzymatic sensing with organic electrochemical transistors , 2008 .

[13]  E. Chan,et al.  High-performance liquid chromatographic assay for catecholamines and metanephrines using fluorimetric detection with pre-column 9-fluorenylmethyloxycarbonyl chloride derivatization. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[14]  Anil Kumar,et al.  Novel label-free DNA sensors based on poly(3,4-ethylenedioxythiophene). , 2004, Chemical communications.

[15]  Changcheng Zhu,et al.  A simple poly(3,4-ethylene dioxythiophene)/poly(styrene sulfonic acid) transistor for glucose sensing at neutral pH. , 2004, Chemical communications.

[16]  Milton L. Lee,et al.  Determination of catecholamines and metanephrines in urine by capillary electrophoresis-electrospray ionization-time-of-flight mass spectrometry. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[17]  Cecilia Lete,et al.  Electrochemical sensors based on platinum electrodes modified with hybrid inorganic–organic coatings for determination of 4-nitrophenol and dopamine , 2009 .

[18]  Henry S. White,et al.  Chemical derivatization of an array of three gold microelectrodes with polypyrrole: Fabrication of a molecule-based transistor , 1984 .

[19]  Yuzhong Zhang,et al.  Study on the electrochemical behavior of dopamine with poly(sulfosalicylic acid) modified glassy carbon electrode , 2001 .

[20]  Feng Yan,et al.  Improvement of the tunable wettability property of poly(3-alkylthiophene) films. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[21]  S. Laviolette.  Dopamine modulation of emotional processing in cortical and subcortical neural circuits: evidence for a final common pathway in schizophrenia? , 2007, Schizophrenia bulletin.

[22]  K. Ho,et al.  Enhancing dopamine detection using a glassy carbon electrode modified with MWCNTs, quercetin, and Nafion. , 2009, Biosensors & bioelectronics.

[23]  George G Malliaras,et al.  Integration of a surface-directed microfluidic system with an organic electrochemical transistor array for multi-analyte biosensors. , 2009, Lab on a chip.

[24]  C. Su,et al.  Ultrasensitive detection of dopamine using a polysilicon nanowire field-effect transistor. , 2008, Chemical communications.

[25]  Anilesh Kumar,et al.  Conductimetric immunosensor based on poly(3,4-ethylenedioxythiophene). , 2002, Chemical communications.

[26]  J. Ahlskog.  Beating a dead horse Dopamine and Parkinson disease , 2007 .

[27]  Feng Yan,et al.  Ion-sensitive properties of organic electrochemical transistors. , 2010, ACS applied materials & interfaces.

[28]  Feng Yan,et al.  Application of thin-film transistors in label-free DNA biosensors , 2010, Expert review of molecular diagnostics.

[29]  Feng Yan,et al.  Label-free DNA sensor based on organic thin film transistors. , 2009, Biosensors & bioelectronics.

[30]  Gengfeng Zheng,et al.  Detection, Stimulation, and Inhibition of Neuronal Signals with High-Density Nanowire Transistor Arrays , 2006, Science.

[31]  Feng Yan,et al.  The Application of Organic Electrochemical Transistors in Cell‐Based Biosensors , 2010, Advanced materials.

[32]  S. Ikemoto.  Dopamine reward circuitry: Two projection systems from the ventral midbrain to the nucleus accumbens–olfactory tubercle complex , 2007, Brain Research Reviews.

[33]  M. Hows,et al.  High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples , 2004, Journal of Neuroscience Methods.

[34]  Ilya Sychugov,et al.  Surface charge sensitivity of silicon nanowires: size dependence. , 2007, Nano letters.

[35]  Feng Yan,et al.  Highly Sensitive Glucose Biosensors Based on Organic Electrochemical Transistors Using Platinum Gate Electrodes Modified with Enzyme and Nanomaterials , 2011 .