Effect of structural and physico-chemical features of cellulosic substrates on the efficiency of enzymatic hydrolysis

Effects of major physicochemical and structural parameters of cellulose on the rate and degree of its enzymatic hydrolysis were tested with cellulosic materials from various sources. Some different pretreatments were: mechanical (milling), physical (X-ray irradiation), and chemical (cadoxen, H3PO4, H2SO4, NaOH, Fe2+/H2O2). The average size of cellulose particles and its degree of polymerization had little effect on the efficiency of enzymatic hydrolysis. For samples of pure cellulose (cotton linter, microcrystalline cellulose, α-cellulose), increase in the specific surface area accessible to protein molecules and decrease in the crystallinity index accelerated the enzymatic hydrolysis (the correlation coefficients were 0.89 and 0.92, respectively). In the case of lignocellulose (bagasse), a quantitative linear relationship only between specific surface area and reactivity was observed.

[1]  Emil Ott,et al.  Cellulose and cellulose derivatives , 1954 .

[2]  M. Rabinowitch,et al.  Enzymatic Conversion of Cellulose to Glucose: Present State of the Art and Potential , 1980 .

[3]  A. Klyosov,et al.  Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan , 1988 .

[4]  M. Himmel,et al.  Hydrodynamics and protein hydration. , 1979, Archives of biochemistry and biophysics.

[5]  A. Converse,et al.  Surface area of pretreated lignocellulosics as a function of the extent of enzymatic hydrolysis , 1989 .

[6]  M. Mandels,et al.  Cellulases: Biosynthesis and applications , 1980 .

[7]  L. Fan,et al.  Kinetic studies of enzymatic hydrolysis of insoluble cellulose: Analysis of the initial rates , 1982, Biotechnology and bioengineering.

[8]  Takashi Sasaki,et al.  Correlation between X‐ray diffraction measurements of cellulose crystalline structure and the susceptibility to microbial cellulase , 1979 .

[9]  R. Atalla.  The Structures of cellulose : characterization of the solid states , 1987 .

[10]  P. Weimer,et al.  Relationship between the fine structure of native cellulose and cellulose degradability by the cellulase complexes of Trichoderma reesei and Clostridium thermocellum , 1985, Biotechnology and bioengineering.

[11]  J. E. Stone,et al.  Digestibility as a Simple Function of a Molecule of Similar Size to a Cellulase Enzyme , 1969 .

[12]  B. Dale,et al.  Enzymatic hydrolysis and recrystallization behavior of initially amorphous cellulose , 1985, Biotechnology and bioengineering.

[13]  P. Carniti,et al.  Cellulosic materials: Structure and enzymatic hydrolysis relationships , 1984 .

[14]  M. Ladisch,et al.  Effect of pretreatments and fermentation on pore size in cellulosic materials , 1985, Biotechnology and bioengineering.

[15]  Sun Bok Lee,et al.  Effect of compression milling on cellulose structure and on enzymatic hydrolysis kinetics , 1982, Biotechnology and bioengineering.

[16]  L. Segal',et al.  An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer , 1959 .

[17]  J. Shewale,et al.  Enzymatic hydrolysis of cellulosic materials by Sclerotium rolfsii culture filtrate for sugar production. , 1979, Canadian journal of microbiology.

[18]  A. Gusakov,et al.  Factors affecting the enzymatic hydrolysis of cellulose in batch and continuous reactors: computer simulation and experiment. , 1987, Biotechnology and bioengineering.

[19]  G. Emert,et al.  Factors affecting the enzymatic hydrolysis of municipal-solid-waste components. , 1988, Biotechnology and bioengineering.

[20]  M. M. Gharpuray,et al.  Structural modification of lignocellulosics by pretreatments to enhance enzymatic hydrolysis , 1983, Biotechnology and bioengineering.

[21]  G. Palade,et al.  INTESTINAL CAPILLARIES I. Permeability to Peroxidase and Ferritin , 1969 .

[22]  L. Fan,et al.  Mechanism of the enzymatic hydrolysis of cellulose: Effects of major structural features of cellulose on enzymatic hydrolysis , 1980 .

[23]  H. Taguchi,et al.  Structural properties of cellulose and cellulase reaction mechanism , 1983, Biotechnology and bioengineering.

[24]  V. Puri.  Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification , 1984, Biotechnology and bioengineering.