Electrochemical studies on tetrathiafulvalene-tetracyanoquinodimethane modified acetylcholine/choline sensor

A sensor for acetylcholine/choline is described using a tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) electrode modified with acetylcholine esterase (AChE) and choline oxidase (ChO) enzymes. DC cyclic voltammetry and impedance measurements of the enzyme-modified TTF-TCNQ electrode that indicate the regeneration of choline oxidase at the electrode surface are reported. Effective electrochemical rate constants for the present enzyme electrode are calculated using the expressions derived by Albery et al. (1), which show the enzyme kinetics as the rate-limiting step. The values of the effective electrochemical rate constants are close to those reported by Hale and Wightman (2). The application of the sensor is described for the determination of fluorode ion and nicotine based on the reversible inhibition of AChE activity. The range of detection of fluoride ion and nicotine is found to be 5×10-6 to 5×10-4M.

[1]  K. Nagai,et al.  Electrical stimulation of the suprachiasmatic nucleus of the hypothalamus causes hyperglycemia. , 1988, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.

[2]  T. Yao.  Flow injection analysis for cholinesterase in blood serum by use of a choline-sensitive electrode as an amperometric detector , 1983 .

[3]  Hollander,et al.  Evaluation of a colorimetric method for determination of glycosylated hemoglobin. , 1983, Clinical chemistry.

[4]  K. Yoda,et al.  Multi-enzyme membrane electrodes for determination of creatinine and creatine in serum. , 1983, Clinical chemistry.

[5]  J. Kulys,et al.  Biocatalytic oxidation of glucose on the conductive charge transfer complexes , 1981 .

[6]  J. B. Justice,et al.  Voltammetric recording from neostriatum of behaving rhesus monkey , 1981, Brain Research.

[7]  Wayne S. Lindsay,et al.  Microcomputer Controlled Multielectrode System for in Vivo Electrochemistry , 1980 .

[8]  G. S. Wilson,et al.  Enzyme electrocatalysis at organic salt electrodes , 1988 .

[9]  J. Kulys,et al.  Oxidation of glucose oxidase from Penicillium vitale by one- and two-electron acceptors , 1983 .

[10]  J. Salamone,et al.  Acetycholine and choline ion-selective microelectrodes , 1983 .

[11]  Adam Heller,et al.  Direct electrical communication between chemically modified enzymes and metal electrodes. I. Electron transfer from glucose oxidase to metal electrodes via electron relays, bound covalently to the enzyme , 1987 .

[12]  J. Kulys,et al.  Reagentless lactate sensor based on cytochrome b2 , 1980 .

[13]  J. J. Kalys.  Enzyme electrodes based on organic metals , 1986 .

[14]  S L Mayo,et al.  Long-range electron transfer in heme proteins. , 1986, Science.

[15]  P. Bartlett,et al.  Amperometric enzyme electrodes. , 1987, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[16]  F. Mizutani,et al.  Amperometric determination of cholinesterase with use of an immobilized enzyme electrode , 1982 .

[17]  P C Pandey,et al.  Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase. , 1990, Biosensors & bioelectronics.

[18]  M. Mascini,et al.  AMPEROMETRIC ACETYLCHOLINE AND CHOLINE SENSORS WITH IMMOBILIZED ENZYMES , 1986 .

[19]  A. Brajter-toth,et al.  Tetrathiafulvalene tetracyanoquinodimethane xanthine oxidase amperometric electrode for the determination of biological purines. , 1987, Analytical chemistry.

[20]  Philip N. Bartlett,et al.  Electrochemical sensors: theory and experiment , 1986 .

[21]  A. Brajter-toth,et al.  Electrochemical behavior of small biological molecules at organic donor―acceptor electrodes in aqueous media , 1988 .

[22]  R. Wightman,et al.  Enzyme-Modified Tetrathiafulvalene Tetracyanoquinodimethane Microelectrodes: Direct Amperometric Detection of Acetylcholine and Choline , 1988 .

[23]  M. Freund,et al.  Ultramicroelectrode array behavior of one-dimensional organic conductor electrodes. , 1989, Analytical chemistry.

[24]  Philip N. Bartlett,et al.  Amperometric enzyme electrodes: Part I. Theory , 1985 .

[25]  I. Karube,et al.  Amperometric determination of phosphatidyl choline in serum with use of immobilized phospholipase d and choline oxidase , 1979 .

[26]  J. Knowles,et al.  Evolution of enzyme function and the development of catalytic efficiency. , 1976, Biochemistry.