Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro

Background and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. The cells without addition of stretching force were used as controls. The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. Furthermore, expressions of HSP70-, VEGF-, and OPN-mRNAs of cells were also evaluated using quantitative RT-PCR. Results. Actin filaments were randomly orientated in the cytoplasm in the control group, whereas in the stretching group, actin filaments were orientated comparatively parallel to the stretching direction. Expression of HSP70-mRNA in the stretching group was significantly higher than that of control group at 12, 24, 36 hours (P < .05). Expression of VEGF-mRNA in the stretching group was significantly higher than that of control group at 24, 36, 48, and 72 hours (P < .05). Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P < .05). Conclusion. ERM cells response against the stretching force by expressing HSP70, VEGF, and OPN.

[1]  W. Marsden.  I and J , 2012 .

[2]  K. Sakurai,et al.  Morphological and molecular changes in denture-supporting tissues under persistent mechanical stress in rats. , 2008, Journal of oral rehabilitation.

[3]  T. Diekwisch,et al.  RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model. , 2008, European journal of oral sciences.

[4]  Y. Yamaguchi,et al.  In vitro characterization of the cytokine profile of the epithelial cell rests of Malassez. , 2008, Journal of periodontology.

[5]  W. Young,et al.  The epithelial cell rests of Malassez--a role in periodontal regeneration? , 2006, Journal of periodontal research.

[6]  M. Asaka,et al.  Protective effects of heat shock protein 70 induced by geranylgeranylacetone on oxidative injury in rat intestinal epithelial cells , 2006, Scandinavian journal of gastroenterology.

[7]  W. Young,et al.  Production of osteopontin by cultured porcine epithelial cell rests of Malassez. , 2005, Journal of periodontal research.

[8]  Guang-shun Yang,et al.  Oral administration of geranylgeranylacetone plus local somatothermal stimulation: a simple, effective, safe and operable preconditioning combination for conferring tolerance against ischemia-reperfusion injury in rat livers. , 2005, World journal of gastroenterology.

[9]  K Soma,et al.  Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress. , 2005, Journal of periodontal research.

[10]  H. Shiba,et al.  Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone‐related and enamel proteins , 2005, Cell biology international.

[11]  V. Gabai,et al.  Protein aggregation as primary and characteristic cell reaction to various stresses , 1993, Experientia.

[12]  B. Melsen,et al.  Tissue reaction to orthodontic tooth movement in acute and chronic corticosteroid treatment. , 2004, Orthodontics & craniofacial research.

[13]  M. Shimono,et al.  Pulp cell responses during hypoxia and reoxygenation in vitro. , 2003, European journal of oral sciences.

[14]  M. Cascales,et al.  Relationship between expression of HSP70 and metallothionein and oxidative stress during mercury chloride induced acute liver injury in rats , 2003, Journal of biochemical and molecular toxicology.

[15]  M. Shimono,et al.  Regulatory mechanisms of periodontal regeneration , 2003, Microscopy research and technique.

[16]  H. Kurihara,et al.  Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair. , 2003, Journal of periodontal research.

[17]  H. Beere.  Stressed to Death: Regulation of Apoptotic Signaling Pathways by the Heat Shock Proteins , 2001, Science's STKE.

[18]  A. E. El Haj,et al.  Selected contribution: regulatory pathways involved in mechanical induction of c-fos gene expression in bone cells. , 2000, Journal of applied physiology.

[19]  A. Bolcato-Bellemin,et al.  Expression of mRNAs Encoding for α and β Integrin Subunits, MMPs, and TIMPs in Stretched Human Periodontal Ligament and Gingival Fibroblasts , 2000 .

[20]  Dick D. Mosser,et al.  Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome , 2000, Nature Cell Biology.

[21]  A. Bolcato-Bellemin,et al.  Expression of mRNAs encoding for alpha and beta integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts. , 2000, Journal of dental research.

[22]  S. Abe,et al.  Vascular endothelial growth factor and osteopontin in tumor biology. , 2000, Critical reviews in oncogenesis.

[23]  S. Takeshita,et al.  Role of epidermal growth factor and its receptor in mechanical stress-induced differentiation of human periodontal ligament cells in vitro. , 1998, Archives of oral biology.

[24]  Y. Hamamoto,et al.  Morphological changes of epithelial rests of Malassez in rat molars induced by local administration of N-methylnitrosourea. , 1998, Archives of oral biology.

[25]  Y. Hamamoto,et al.  Production of amelogenin by enamel epithelium of Hertwig's root sheath. , 1996, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[26]  D. Welling,et al.  Repair of chronic tympanic membrane perforations with long‐term epidermal growth factor , 1995, The Laryngoscope.

[27]  K. Alitalo,et al.  Vascularization of the mouse embryo: A study of flk‐1, tek, tie, and vascular endothelial growth factor expression during development , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[28]  H. Dvorak,et al.  Vascular permeability factor/vascular endothelial growth factor: an important mediator of angiogenesis in malignancy and inflammation. , 1995, International archives of allergy and immunology.

[29]  R. Comolli,et al.  Differential expression of c-jun, c-fos and hsp 70 mRNAs after folic acid and ischemia-reperfusion injury: effect of antioxidant treatment. , 1994, Experimental nephrology.

[30]  C Neidlinger-Wilke,et al.  Cyclic stretching of human osteoblasts affects proliferation and metabolism: A new experimental method and its application , 1994, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[31]  T. Tsang.  New model for 70 kDa heat‐shock proteins' potential mechanisms of function , 1993, FEBS letters.

[32]  D. van Steenberghe,et al.  Periodontal neural endings intimately relate to epithelial rests of Malassez in humans. A light and electron microscope study. , 1993, Journal of anatomy.

[33]  D. Goeddel,et al.  Vascular endothelial growth factor is a secreted angiogenic mitogen. , 1989, Science.

[34]  Y. Hamamoto,et al.  Ultrastructural and histochemical study on the morphogenesis of epithelial rests of Malassez. , 1989, Archives of histology and cytology.

[35]  Y. Hamamoto,et al.  Ultrastructure of epithelial rests of Malassez in human periodontal ligament. , 1989, Archives of oral biology.

[36]  H. Hämmerle,et al.  Orientation response of arterial smooth muscle cells to mechanical stimulation. , 1986, European journal of cell biology.

[37]  J. D. Spouge.  A study of epithelial odontogenic residues in the pig. , 1986, Journal of periodontology.

[38]  D. Brunette,et al.  Mechanical stretching increases the number of epithelial cells synthesizing DNA in culture. , 1984, Journal of cell science.

[39]  J. D. Spouge.  A new look at the rests of Malassez. A review of their embryological origin, anatomy, and possible role in periodontal health and disease. , 1980, Journal of periodontology.

[40]  R. Buck,et al.  Reorientation response of cells to repeated stretch and recoil of the substratum. , 1980, Experimental cell research.

[41]  D. Brunette,et al.  In-vitro cultural parameters and protein and prostaglandin secretion of epithelial cells derived from porcine rests of Malassez. , 1979, Archives of oral biology.

[42]  A. T. Ten Cate.  The epithelial cell rests of Malassez and the genesis of the dental cyst. , 1972, Oral surgery, oral medicine, and oral pathology.

[43]  L. Fieser.  THE SYNTHESIS OF VITAMIN K1. , 1940, Science.