Effect of Length of Time before Incorporation on Survival of Pathogenic Bacteria Present in Livestock Wastes Applied to Agricultural Soil

ABSTRACT In response to reports that the contamination of food can occur during the on-farm primary phase of food production, we report data that describes a possible cost-effective intervention measure. The effect of time before soil incorporation of livestock wastes spread to land on the rate of decline of zoonotic agents present in the waste was investigated. Fresh livestock wastes were inoculated with laboratory-cultured Salmonella, Listeria, and Campylobacter spp. and Escherichia coli O157 before they were spead onto soil. Incorporation of the spread wastes was either immediate, delayed for 1 week, or did not occur at all. Bacterial decline was monitored over time and found to be significantly more rapid for all waste types when they were left on the soil surface. There were no significant differences in initial bacterial decline rates when wastes were spread in summer or winter. Our results indicate that not incorporating contaminated livestock wastes into soil is a potential intervention measure that may help to limit the spread of zoonotic agents further up the food chain. The implications of these findings are discussed in relation to current advice for livestock waste disposal.

[1]  S. Baloda,et al.  Persistence of a Salmonella entericaSerovar Typhimurium DT12 Clone in a Piggery and in Agricultural Soil Amended with Salmonella-Contaminated Slurry , 2001, Applied and Environmental Microbiology.

[2]  R. Chalmers,et al.  Waterborne Escherichia coli O157 , 2000, Symposium series.

[3]  M. Doyle,et al.  Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces , 1996, Applied and environmental microbiology.

[4]  K. A. Smith,et al.  A survey of the production and use of animal manures in England and Wales , 2001 .

[5]  H. Watanabe,et al.  Verotoxin‐producing Escherichia coli O157:H7 carried by the housefly in Japan , 1999, Medical and veterinary entomology.

[6]  E. Barbour,et al.  Soil Solarization: A Sustainable Agriculture Approach to Reduce Microorganisms in Chicken Manure-Treated Soil , 2002 .

[7]  T. Morvan,et al.  Dynamics of Pig Slurry Nitrogen in Soil and Plant as Determined with 15N , 2004 .

[8]  A. Pell.  Manure and Microbes: Public and Animal Health Problem? , 1997, Journal of Dairy Science.

[9]  R. Holley,et al.  Transmission and control of Escherichia coli O157:H7 — A review , 2002 .

[10]  B. Chambers,et al.  A study on farm manure applications to agricultural land and an assessment of the risks of pathogen transfer into the food chain. A report to the Ministry of Agricultural Fisheries and Food, January 2000. , 2000 .

[11]  K. P. Flint,et al.  The effects of nutrients on the survival of Escherichia coli in lake water , 1989 .

[12]  J. Hadler,et al.  A multistate outbreak of Escherichia coli O157:H7 infections associated with consumption of mesclun lettuce. , 1999, Archives of internal medicine.

[13]  D. Fenlon,et al.  Fate of E.coli and E.coli O157 in soils and drainage water following cattle slurry application at 3 sites in southern Scotland , 2002 .

[14]  N. Hansen,et al.  Persistence of zoonotic pathogens in surface soil treated with different rates of liquid pig manure , 2004 .

[15]  Haruo Watanabe,et al.  Houseflies: not simple mechanical vectors of enterohemorrhagic Escherichia coli O157:H7. , 1999, The American journal of tropical medicine and hygiene.

[16]  M. Doyle,et al.  Fate of Escherichia coli O157:H7 in Manure-Amended Soil , 2002, Applied and Environmental Microbiology.

[17]  Robert E. Brackett,et al.  Incidence, contributing factors, and control of bacterial pathogens in produce , 1999 .

[18]  G. Kapperud,et al.  Epidemiology of Salmonella typhimurium O:4-12 infection in Norway: evidence of transmission from an avian wildlife reservoir. , 1998, American journal of epidemiology.

[19]  K. P. Flint,et al.  The effects of nutrients on the survival of Escherichia coli in lake water. , 1989, The Journal of applied bacteriology.

[20]  J. Karns,et al.  Leaching of Escherichia coli O157:H7 in Diverse Soils under Various Agricultural Management Practices , 2000, Applied and Environmental Microbiology.

[21]  Paul J. Cieslak,et al.  Escherichia coli 0157:H7 infection from a manured garden , 1993, The Lancet.

[22]  D. Bolton,et al.  The‐survival‐characteristics‐of‐a‐non‐toxigenic‐strain‐of‐Escherichia‐coli‐O157:H7 , 1999, Journal of applied microbiology.

[23]  P. Ciria,et al.  SE—Structure and Environment: Performance Characteristics of Three Aeration Systems in the Composting of Sheep Manure and Straw , 2001 .

[24]  A. Adesiyun,et al.  SOME BACTERIAL ENTEROPATHOGENS IN WILDLIFE AND RACING PIGEONS FROM TRINIDAD , 1998, Journal of wildlife diseases.

[25]  Marcel H Zwietering,et al.  Quantifying recontamination through factory environments--a review. , 2003, International journal of food microbiology.

[26]  P D O'Neill,et al.  Bayesian methods for estimating pathogen prevalence within groups of animals from faecal-pat sampling. , 2003, Preventive veterinary medicine.

[27]  K. Smith,et al.  A survey of the production and use of animal manures in England and Wales. I. Pig manure , 2000 .

[28]  P. W. Jones.  The effect of temperature, solids content and pH on the survival of salmonellas in cattle slurry. , 1976, The British veterinary journal.

[29]  Michael J. Pelczar,et al.  Microbiology: Concepts and Applications , 1993 .

[30]  D. Strauch,et al.  Hygienic aspects of the production and agricultural use of animal wastes. , 1994, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B.

[31]  M. Hutchison,et al.  Levels of zoonotic agents in British livestock manures , 2004, Letters in applied microbiology.

[32]  R. Mandrell,et al.  Colonization of Arabidopsis thaliana with Salmonella enterica and Enterohemorrhagic Escherichia coli O157:H7 and Competition by Enterobacter asburiae , 2003, Applied and Environmental Microbiology.

[33]  K. Kelly,et al.  CD1 antibody immunolocalisation in Langerhans' cell histiocytosis , 1993, The Lancet.

[34]  S. Goyal,et al.  Pathogens and manure management systems: A review , 2003, Environmental technology.

[35]  A. Maule.  Survival of verocytotoxigenic Escherichia coli O157 in soil, water and on surfaces , 2000, Symposium series.