Development of a foam flotation system for harvesting microalgae biomass

Abstract The lack of efficient and cost-effective technologies for harvesting bulk quantities of microalgae biomass is a major obstacle to commercialisation of algae-derived biofuels. This article demonstrates the efficacy of a foam harvester that combines dispersed air flotation with foam fractionation to allow harvesting, concentration, and physical separation of particles in suspension. Fractional factorial experiments using polystyrene latex beads were combined with trials using microalgae to determine the relative importance of key design and operational variables (air flow rate, batch run time, foam column height, surfactant concentration, and surfactant type) on the particle concentration factor. The model revealed that highest concentration factors were gained using the following variables and variable interactions: cationic cetyl trimethylammonium bromide (CTAB), lower surfactant concentrations, and CTAB combined with high column heights. Variables that increased foam residence time produced the greatest concentration factors. Analyses of the harvest economics revealed that foam flotation consumes only 0.015 kWh/m 3 providing an advantageous cost–benefit relationship, and outcompeting many commonly used bulk harvesting technologies.

[1]  G. Narsimhan,et al.  Effects of kinetics of adsorption and coalescence on continuous foam concentration of proteins: Comparison of experimental results with model predictions , 2000, Biotechnology and bioengineering.

[2]  De-hua Liu,et al.  Perspectives of microbial oils for biodiesel production , 2008, Applied Microbiology and Biotechnology.

[3]  Stephen J. Neethling,et al.  A novel approach for estimating the average bubble size for foams flowing in vertical columns , 2004 .

[4]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[5]  Michael K. Danquah,et al.  Dewatering of microalgal culture for biodiesel production: exploring polymer flocculation and tangential flow filtration , 2009 .

[6]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[7]  Victoria O Adesanya,et al.  The rheological characterization of algae suspensions for the production of biofuels , 2012 .

[8]  Y. Chisti,et al.  Recovery of microalgal biomass and metabolites: process options and economics. , 2003, Biotechnology advances.

[9]  Stephen J. Neethling Simple approximations for estimating froth recovery , 2008 .

[10]  S. Razavi,et al.  Use of response surface methodology in a fed-batch process for optimization of tricarboxylic acid cycle intermediates to achieve high levels of canthaxanthin from Dietzia natronolimnaea HS-1. , 2010, Journal of bioscience and bioengineering.

[11]  Andrew T. Csordas,et al.  An integrated photobioreactor and foam fractionation unit for the growth and harvest of Chaetoceros spp. in open systems , 2004 .

[12]  Yi-Hsu Ju,et al.  Separation of Algal Cells from Water by Column flotation , 1999 .

[13]  D. A. White,et al.  Removal of algae using froth flotation , 2003, Environmental technology.

[14]  W. Brown,et al.  Adsorption of sodium dodecyl sulfate on polystyrene latex particles using dynamic light scattering and zeta potential measurements , 1993 .

[15]  S. Chavadej,et al.  Anionic and cationic surfactant recovery from water using a multistage foam fractionator , 2003 .

[16]  René H. Wijffels,et al.  Ultrasound, a new separation technique to harvest microalgae , 2003, Journal of Applied Phycology.

[17]  M. Borowitzka Commercial production of microalgae: ponds, tanks, tubes and fermenters , 1999 .

[18]  Stephen J. Neethling,et al.  A foam drainage equation generalized for all liquid contents , 2002 .

[19]  H. Oh,et al.  Harvesting of Spirulina platensis by cellular flotation and growth stage determination , 2005, Letters in applied microbiology.

[20]  Annabeth L. Propst,et al.  Designing for Quality: An introduction to the best of Taguchi and Western methods of statistical experimental design , 1990 .

[21]  B. Jefferson,et al.  Surfactants as bubble surface modifiers in the flotation of algae: dissolved air flotation that utilizes a chemically modified bubble surface. , 2008, Environmental science & technology.

[22]  M. Demirbas,et al.  IMPORTANCE OF ALGAE OIL AS A SOURCE OF BIODIESEL , 2011 .

[23]  S. Paria,et al.  A review on experimental studies of surfactant adsorption at the hydrophilic solid-water interface. , 2004, Advances in colloid and interface science.

[24]  Y. Ju,et al.  FLOTATION REMOVAL OF ALGAE FROM WATER , 1998 .

[25]  N. De Pauw,et al.  Potential of electrolytic flocculation for recovery of micro-algae , 1997 .

[26]  Perry D. Haaland,et al.  Experimental design in biotechnology , 1989 .

[27]  D. A. White,et al.  Comparison between the removal of live and dead algae using froth flotation , 2005 .

[28]  U. Wiesmann,et al.  SINGLE AND MULTISTAGE FOAM FRACTIONATION OF RINSE WATER WITH ALKYL ETHOXYLATE SURFACTANTS , 2001 .

[29]  J. Varley,et al.  Continuous foaming for protein recovery: Part I. Recovery of β‐casein , 1999 .

[30]  A. Jacobson,et al.  Improved Algal Harvesting Using Suspended Air Flotation , 2009, Water environment research : a research publication of the Water Environment Federation.

[31]  O. Pulz,et al.  Valuable products from biotechnology of microalgae , 2004, Applied Microbiology and Biotechnology.

[32]  V. Z̆utić,et al.  Surfactant production by marine phytoplankton , 1981 .

[33]  W. Maher,et al.  Measurement of arsenic species in marine macroalgae by microwave-assisted extraction and high performance liquid chromatography-inductively coupled plasma mass spectrometry , 2002 .

[34]  Arnaud Hélias,et al.  Life-cycle assessment of biodiesel production from microalgae. , 2009, Environmental science & technology.

[35]  X. Mari,et al.  Metal induced variations of TEP sticking properties in the southwestern lagoon of New Caledonia , 2008 .

[36]  C. Lan,et al.  CO2 bio-mitigation using microalgae , 2008, Applied Microbiology and Biotechnology.

[37]  Andrew Hoadley,et al.  Dewatering of microalgal cultures : a major bottleneck to algae-based fuels , 2010 .

[38]  J. Cilliers,et al.  Modelling flotation froths , 2003 .

[39]  J. Benemann,et al.  Look Back at the U.S. Department of Energy's Aquatic Species Program: Biodiesel from Algae; Close-Out Report , 1998 .

[40]  Michael K. Danquah,et al.  Microalgal growth characteristics and subsequent influence on dewatering efficiency , 2009 .

[41]  E. Becker Microalgae: Biotechnology and Microbiology , 1994 .

[42]  C. Laluce,et al.  Effects of organic and inorganic additives on flotation recovery of washed cells of Saccharomyces cerevisiae resuspended in water. , 2006, Colloids and surfaces. B, Biointerfaces.

[43]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .