The fine structure of wound repair in an insect (Rhodnius prolixus)

The cells surrounding a wound in the integument of Rhodnius adults show an increase in RNA content, cytochrome oxidase and esterase activity. An excision in the integument is filled by blood which coagulates and is tanned into an insoluble membrane. The basement membrane of the adjoining epidermis acts as a self‐sealing membrane and contracts to cover the excision. The epidermis is attached to the cuticle by the subcuticular layer which it resorbs and by pore canal filaments which are left behind as it migrates. The epidermis migrates as a sheet in contact with the cuticle then with the coagulated blood and basement membrane which cover the excision. Blood cells migrate individually into an excision and do not adhere to a surface in the process. Microtubules cannot be identified with movement. Both epidermal and blood cells remove the cells killed by wounding as evidenced by the appearance of coated vesicles and phagocytic bodies in both cell types. The reconstituted integument consists of a surface membrane in which the layers of the epicuticle are not distinguishable, a nonlamellate cuticle secreted by an epidermis which also appears to secrete the new basement membrane.

[1]  W. Harvey,et al.  CELLULAR MEMBRANE FORMATION BY PLASMATOCYTES OF DIAPAUSING CECROPIA PUPAE. , 1965, Journal of insect physiology.

[2]  M. Locke.  PORE CANALS AND RELATED STRUCTURES IN INSECT CUTICLE , 1961, The Journal of biophysical and biochemical cytology.

[3]  B. Stay.  PROTEIN UPTAKE IN THE OOCYTES OF THE CECROPIA MOTH , 1965, The Journal of cell biology.

[4]  E. L. Schmidt.  Observations on the subcuticular layer in the insect integument , 1956 .

[5]  C. Kurland,et al.  THE RESPIRATORY ENZYMES OF DIAPAUSING SILKWORM PUPAE: A NEW INTERPRETATION OF CARBON MONOXIDE-INSENSITIVE RESPIRATION , 1959 .

[6]  A. D. Reuck,et al.  Ciba Foundation Symposium - Lysosomes , 1963 .

[7]  V. Wigglesworth.  Insect Blood Cells , 1959 .

[8]  K. Porter,et al.  A "MICROTUBULE" IN PLANT CELL FINE STRUCTURE , 1963, The Journal of cell biology.

[9]  P. Weiss,et al.  Electronmicrograms of larval amphibian epidermis. , 1954, Experimental cell research.

[10]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[11]  K. Porter,et al.  YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L , 1964, The Journal of cell biology.

[12]  J. Lai‐Fook.  The structure of developing muscle insertions in insects , 1967, Journal of morphology.

[13]  V. Wigglesworth.  Transpiration Through the Cuticle of Insects , 1945 .

[14]  G. de‐Thé,et al.  CYTOPLASMIC MICROTUBULES IN DIFFERENT ANIMAL CELLS , 1964, The Journal of cell biology.

[15]  R. Pipa,et al.  The structure and histochemistry of the connective tissue of the sucking lice , 1958 .

[16]  A. Richards,et al.  AN AUTORADIOGRAPHIC STUDY OF THE RELATION BETWEEN HEMOCYTES AND CONNECTIVE TISSUE IN THE WAX MOTH, GALLERIA MELLONELLA L , 1965 .

[17]  J. Beament.  THE WATER RELATIONS OF INSECT CUTICLE , 1961, Biological reviews of the Cambridge Philosophical Society.

[18]  V. Wigglesworth.  The Role of the Haemocytes in the Growth and Moulting of an Insect, Rhodnius Prolixus (Hemiptera) , 1955 .

[19]  M. Locke.  The structure and formation of the cuticulin layer in the epicuticle of an insect, Calpodes ethlius (Lepidoptera, Hesperiidae) , 1966, Journal of morphology.

[20]  V. Wigglesworth,et al.  Wound Healing in an Insect (Rhodnius Prolixus Hemiptera) , 1937 .

[21]  P. Weiss,et al.  The basement lamelia of amphibian skin; its reconstruction after wounding. , 1956, The Journal of biophysical and biochemical cytology.

[22]  H. Schneiderman,et al.  Control of Synthesis of RNA and Protein in Diapausing and Injured Cecropia Pupae , 1964, Science.

[23]  M. Locke.  Secretion of Wax through the Cuticle of Insects , 1959, Nature.

[24]  A. Neville.  Daily Growth Layers for Determining the Age of Grasshopper Populations , 1963 .

[25]  M. Locke,et al.  THE STRUCTURE AND FORMATION OF PROTEIN GRANULES IN THE FAT BODY OF AN INSECT , 1965, The Journal of cell biology.

[26]  G. Palade,et al.  JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA , 1963, The Journal of cell biology.

[27]  K. Porter,et al.  Morphology of Microtubules of Plant Cell , 1964, Science.

[28]  M. Abercrombie.  The bases of the locomotory behaviour of fibroblasts. , 1961, Experimental cell research.

[29]  M. Locke.  THE STRUCTURE OF SEPTATE DESMOSOMES , 1965, The Journal of cell biology.

[30]  G. Salt.  Experimental studies in insect parasitism XI. The haemocytic reaction of a caterpillar under varied conditions , 1960, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[31]  B. Bowers,et al.  PHYSIOLOGY OF INSECT DIAPAUSE. XIII. DNA SYNTHESIS DURING THE METAMORPHOSIS OF THE CECROPIA SILKWORM , 1964 .

[32]  D. E. Kelly.  FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMIS , 1966, The Journal of cell biology.

[33]  Ronald L. Taylor,et al.  Integumentary changes during moulting of arthropods with special reference to the subcuticle and ecdysial membrane , 1965, Journal of morphology.