A new homeobox-containing gene, msh-2, is transiently expressed early during mesoderm formation of Drosophila.

Many homeobox-containing genes of Drosophila regulate pathways of differentiation. These proteins probably function as promoter- or enhancer-selective transcription factors. We have isolated a new homeobox-containing gene, msh-2, by means of the polymerase chain reactions (PCR) using redundant primers. msh-2 is specifically expressed in mesodermal primordia during a short time period early in development. It first appears at blastoderm stage just before the ventral invagination of the mesoderm and shortly after twist, a gene required for mesoderm formation, is expressed. During germband elongation all the mesodermal cells in the segmented part of the embryo express msh-2, but soon afterwards msh-2 becomes restricted to the dorsal mesoderm, which includes the primordia for the visceral musculature and the heart. Prior to muscle differentiation, msh-2 expression ceases, except for two rows of cells that will be included in the dorsal vessel. Embryos that are deficient for the chromosomal region, 93C-F, which includes the msh-2 gene, show normal mesoderm invagination and dorsal spreading. However, later in development no visceral muscle and dorsal vessel differentiation can be detected, but some skeletal muscles do form, albeit abnormally. msh-2 expression, except for a patch in the head, is dependent on twist function. On the other hand, snail, another mesoderm determinant, does not appear to be required for msh-2 initiation, but is necessary for the maintenance of msh-2 expression after germband elongation. H2.0, a homeo-box-containing gene specifically expressed in visceral mesoderm, is not transcribed in the mesoderm in 93C-F deficiency embryos.(ABSTRACT TRUNCATED AT 250 WORDS)

[1]  D. Lindsley,et al.  The Genome of Drosophila Melanogaster , 1992 .

[2]  Harold Weintraub,et al.  The protein Id: A negative regulator of helix-loop-helix DNA binding proteins , 1990, Cell.

[3]  J. Jellies.  Muscle assembly in simple systems , 1990, Trends in Neurosciences.

[4]  H. Weintraub,et al.  The MyoD DNA binding domain contains a recognition code for muscle-specific gene activation , 1990, Cell.

[5]  Y. Jan,et al.  rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster. , 1990, Genes & development.

[6]  J. Lepesant.  [Homeotic genes]. , 1990, Reproduction, nutrition, development.

[7]  C. Nüsslein-Volhard,et al.  A gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo , 1989, Cell.

[8]  M. Noll,et al.  Isolation of two tissue‐specific Drosophila paired box genes, Pox meso and Pox neuro. , 1989, The EMBO journal.

[9]  M. Nirenberg,et al.  Drosophila NK-homeobox genes. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[10]  D. Lockshon,et al.  MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer , 1989, Cell.

[11]  T. Uemura,et al.  Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. , 1989, Genes & development.

[12]  S. Bernstein,et al.  Ultrastructural and molecular analyses of homozygous-viable Drosophila melanogaster muscle mutants indicate there is a complex pattern of myosin heavy-chain isoform distribution. , 1989, Genes & development.

[13]  M. Scott,et al.  The structure and function of the homeodomain. , 1989, Biochimica et biophysica acta.

[14]  R. Renkawitz-Pohl,et al.  Intron and upstream sequences regulate expression of the Drosophila beta 3-tubulin gene in the visceral and somatic musculature, respectively. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[15]  R. Tjian,et al.  Transcription factors and the control of Drosophila development. , 1989, Trends in genetics : TIG.

[16]  M. Levine,et al.  Homeobox proteins as sequence-specific transcription factors , 1988, Cell.

[17]  J. Sninsky,et al.  A sensitive method for the identification of uncharacterized viruses related to known virus groups: hepadnavirus model system. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[18]  P. Ingham.  The molecular genetics of embryonic pattern formation in Drosophila , 1988, Nature.

[19]  W. McGinnis,et al.  A novel, tissue‐specific, Drosophila homeobox gene. , 1988, The EMBO journal.

[20]  B. Thisse,et al.  Sequence of the twist gene and nuclear localization of its protein in endomesodermal cells of early Drosophila embryos. , 1988, The EMBO journal.

[21]  M. Levine.  Molecular analysis of dorsal-ventral polarity in Drosophila , 1988, Cell.

[22]  Y. Jan,et al.  Multiple potassium–channel components are produced by alternative splicing at the Shaker locus in Drosophila , 1988, Nature.

[23]  Sean B. Carroll,et al.  The segmentation and homeotic gene network in early Drosophila development , 1987, Cell.

[24]  Y. Jan,et al.  Transformation of sensory organs by Mutations of the cut locus of D. melanogaster , 1987, Cell.

[25]  B. Thisse,et al.  Genes of the Drosophila maternal dorsal group control the specific expression of the zygotic gene twist in presumptive mesodermal cells , 1987 .

[26]  H. Jäckle,et al.  The gooseberry–zipper region of Drosophila: five genes encode different spatially restricted transcripts in the embryo , 1987, The EMBO journal.

[27]  D. Cavener,et al.  Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. , 1987, Nucleic acids research.

[28]  J. L. Boulay,et al.  The Drosophila developmental gene snail encodes a protein with nucleic acid binding fingers , 1987, Nature.

[29]  T. Kaufman,et al.  Homoeotic Genes and the Specification of Segmental Identity in the Embryo and Adult Thorax of Drosophila Melanogaster , 1984 .

[30]  Y. Jan,et al.  Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and in grasshopper embryos. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[31]  C. Nüsslein-Volhard,et al.  Mutations affecting segment number and polarity in Drosophila , 1980, Nature.

[32]  B. Judd,et al.  Analysis of the Cut Locus of DROSOPHILA MELANOGASTER. , 1979, Genetics.

[33]  E. Lewis.  A gene complex controlling segmentation in Drosophila , 1978, Nature.

[34]  A. C. Crossley,et al.  morphology and development of the Drosophila muscular system , 1978 .

[35]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[36]  Peter Saenger,et al.  THE MORPHOLOGY AND DEVELOPMENT OF , 1971 .