Tandem mass spectrometry of peptides: sequence information based on neutral losses. I: Isomeric dipeptides

The neutral losses arising on the collisionally activated dissociation (CAD) of protonated dipeptides (MH + ) are reionized by collision and detected in neutral fragment-reionization (N f R) mass spectra. For the dipeptides investigated, which encompass Ala-Gly/Gly-Ala, Asp-Gly/Gly-Asp, Phe-Gly/Gly-Phe and Trp-Ala/Ala-Trp, the N f R spectra of each pair of isomers are distinct and permit the identification of the correct C-terminal amino acid. The precise structure of this amino acid is elucidated by comparison of the N f R spectra with reference collision-induced dissociative ionization (CIDI) mass spectra of authentic Gly, Ala, Asp, Phe and Trp, acquired from beams of these amino acids formed by dissociation of their proton-bound dimers

[1]  C. Wesdemiotis,et al.  The neutral products formed during backbone fragmentations of protonated peptides in tandem mass spectrometry. , 1993, Analytical chemistry.

[2]  C. Wesdemiotis,et al.  A new trisector tandem mass spectrometer for neutralization—reionization studies , 1992 .

[3]  D. Cornett,et al.  Controlling the dissociation of peptide ions using laser desorption/chemical ionization Fourier transform mass spectrometry , 1991 .

[4]  R. Cooks,et al.  Angular dependence of internal energy distributions of activated Fe(CO)5.cntdot.+ and W(CO)6.cntdot.+ ions. The contributions of vibrational and electronic excitation mechanisms in kiloelectron volt collisions , 1990 .

[5]  K. Biemann.  Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation. , 1990, Methods in enzymology.

[6]  J. Holmes.  The neutralization of organic cations , 1989 .

[7]  Stephen A. Martin,et al.  Collision-induced fragmentation of (M + H)+ ions of peptides. Side chain specific sequence ions , 1988 .

[8]  Stephen A. Martin,et al.  A comparison of keV atom bombardment mass spectra of peptides obtained with a two-sector mass spectrometer with those from a four-sector tandem mass spectrometer , 1987 .

[9]  F. McLafferty,et al.  Neutralization-reionization mass spectrometry (NRMS) , 1983 .

[10]  F. McLafferty,et al.  Reionization agents for neutralization-reionization mass spectrometry. , 1986, Analytical chemistry.

[11]  P. Burgers,et al.  Collisionally induced dissociative ionization of neutral products from ion fragmentations. 2—Collision gas effects and related phenomena , 1985 .

[12]  J. Szulejko,et al.  Collisionally induced dissociative ionization of neutral products from unimolecular ion fragmentations. 1—Neutral product structures , 1984 .

[13]  Fred W. McLafferty,et al.  Registry of mass spectral data , 1974 .