Split Inteins as Versatile Tools for Protein Semisynthesis

[1]  H. Mootz,et al.  Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing. , 2006, Angewandte Chemie.

[2]  F. Perler,et al.  The mechanism of protein splicing and its modulation by mutation. , 1996, The EMBO journal.

[3]  Teruyuki Nagamune,et al.  Site‐Specific Protein Modification on Living Cells Catalyzed by Sortase , 2008, Chembiochem : a European journal of chemical biology.

[4]  P. Dawson,et al.  Synthesis of native proteins by chemical ligation. , 2000, Annual review of biochemistry.

[5]  F. Bordusa.  Proteases in organic synthesis. , 2002, Chemical reviews.

[6]  Toshio Yamazaki,et al.  Segmental Isotope Labeling for Protein NMR Using Peptide Splicing , 1998 .

[7]  David R. Liu,et al.  Control of transcription factor activity and osteoblast differentiation in mammalian cells using an evolved small-molecule-dependent intein. , 2006, Journal of the American Chemical Society.

[8]  S. Züger,et al.  Highly efficient protein trans‐splicing by a naturally split DnaE intein from Nostoc punctiforme , 2006, FEBS letters.

[9]  M. Coleman,et al.  Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing. , 2006, Angewandte Chemie.

[10]  Y. Ohya,et al.  A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. , 2000, Analytical chemistry.

[11]  T. C. Evans,et al.  Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. , 1999, Gene.

[12]  J. Tom,et al.  A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues. , 1994, Science.

[13]  F. Quiocho,et al.  Crystal Structure of PI-SceI, a Homing Endonuclease with Protein Splicing Activity , 1997, Cell.

[14]  Tom W Muir,et al.  Activation of an autoregulated protein kinase by conditional protein splicing. , 2004, Angewandte Chemie.

[15]  Ming-Qun Xu,et al.  Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein* , 1998, The Journal of Biological Chemistry.

[16]  Xiang‐Qin Liu,et al.  Synthetic Two-piece and Three-piece Split Inteins for Protein trans-Splicing* , 2004, Journal of Biological Chemistry.

[17]  O. Seitz,et al.  Native chemical ligation at valine. , 2008, Angewandte Chemie.

[18]  H. Paulus,et al.  Characteristics of protein splicing in trans mediated by a semisynthetic split intein. , 1999, Biopolymers.

[19]  A. Ting,et al.  Site-specific labeling of proteins with small molecules in live cells. , 2005, Current opinion in biotechnology.

[20]  Y. Kyōgoku,et al.  NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. , 1999, Biochemistry.

[21]  M. Francis,et al.  Transition metal catalyzed methods for site-selective protein modification. , 2006, Current opinion in chemical biology.

[22]  G. Verdine,et al.  The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex. , 1996, Chemistry & biology.

[23]  M. Engelhard,et al.  Semisynthetic murine prion protein equipped with a GPI anchor mimic incorporates into cellular membranes. , 2007, Chemistry & biology.

[24]  Kai Johnsson,et al.  An engineered protein tag for multiprotein labeling in living cells. , 2008, Chemistry & biology.

[25]  J. S. Oeemig,et al.  Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site‐specific chemical modification , 2009, FEBS letters.

[26]  R Y Tsien,et al.  Specific covalent labeling of recombinant protein molecules inside live cells. , 1998, Science.

[27]  Francine B. Perler,et al.  In vitro protein splicing of purified precursor and the identification of a branched intermediate , 1993, Cell.

[28]  D Cowburn,et al.  Segmental isotopic labeling using expressed protein ligation. , 2001, Methods in enzymology.

[29]  N. Johnsson,et al.  Specific labeling of cell surface proteins with chemically diverse compounds. , 2004, Journal of the American Chemical Society.

[30]  S. Chattopadhaya,et al.  In Vivo Imaging of a Bacterial Cell Division Protein Using a Protease‐Assisted Small‐Molecule Labeling Approach , 2008, Chembiochem : a European journal of chemical biology.

[31]  Yi Ding,et al.  Crystal Structure of a Mini-intein Reveals a Conserved Catalytic Module Involved in Side Chain Cyclization of Asparagine during Protein Splicing* , 2003, Journal of Biological Chemistry.

[32]  Teruyuki Nagamune,et al.  Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation. , 2007, Chemical communications.

[33]  E. Buchinger,et al.  In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins , 2009, PloS one.

[34]  T. C. Evans,et al.  Protein trans-Splicing and Cyclization by a Naturally Split Intein from the dnaE Gene ofSynechocystis Species PCC6803* , 2000, The Journal of Biological Chemistry.

[35]  O. Schueler‐Furman,et al.  Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations. , 2007, Biochemistry.

[36]  T. C. Evans,et al.  Semisynthesis of cytotoxic proteins using a modified protein splicing element , 1998, Protein science : a publication of the Protein Society.

[37]  H. Mootz,et al.  Design of an intein that can be inhibited with a small molecule ligand. , 2005, Journal of the American Chemical Society.

[38]  D. Schwarzer,et al.  Interaction Studies and Alanine Scanning Analysis of a Semi-synthetic Split Intein Reveal Thiazoline Ring Formation from an Intermediate of the Protein Splicing Reaction* , 2008, Journal of Biological Chemistry.

[39]  H. Paulus,et al.  Protein splicing and related forms of protein autoprocessing. , 2000, Annual review of biochemistry.

[40]  Henning D. Mootz,et al.  Regioselektive Cysteinbiokonjugation durch Anhängen eines modifizierten Cystein‐tags an ein Protein mithilfe von trans‐Proteinspleißen , 2007 .

[41]  Tom W Muir,et al.  Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo. , 2003, Journal of the American Chemical Society.

[42]  K Morikawa,et al.  Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI. , 2000, Journal of molecular biology.

[43]  F. Perler,et al.  Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. , 1997, Gene.

[44]  D. Schwarzer,et al.  Chemoselective ligation and modification strategies for peptides and proteins. , 2008, Angewandte Chemie.

[45]  C. Bertozzi,et al.  Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes , 2007, Nature Biotechnology.

[46]  M. Southworth,et al.  An alternative protein splicing mechanism for inteins lacking an N‐terminal nucleophile , 2000, The EMBO journal.

[47]  T. C. Evans,et al.  Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. , 1999, BioTechniques.

[48]  R. Hirata,et al.  Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. , 1990, The Journal of biological chemistry.

[49]  Teruyuki Nagamune,et al.  Sortase‐Mediated Ligation: A Gift from Gram‐Positive Bacteria to Protein Engineering , 2009, Chembiochem : a European journal of chemical biology.

[50]  K. Zhou,et al.  Novel Split Intein for trans-Splicing Synthetic Peptide onto C Terminus of Protein* , 2009, Journal of Biological Chemistry.

[51]  S. Hart,et al.  Sortase-mediated protein ligation: a new method for protein engineering. , 2004, Journal of the American Chemical Society.

[52]  T. Muir,et al.  Activation of protein splicing by protease- or light-triggered O to N acyl migration. , 2008, Angewandte Chemie.

[53]  J. Camarero,et al.  Synthesis of proteins by native chemical ligation using Fmoc-based chemistry. , 2005, Protein Peptide Letters.

[54]  H. Mootz,et al.  Engineering artificially split inteins for applications in protein chemistry: biochemical characterization of the split Ssp DnaB intein and comparison to the split Sce VMA intein. , 2006, Biochemistry.

[55]  Z. Hu,et al.  Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[56]  H. Wu,et al.  Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. , 1998, Biochimica et biophysica acta.

[57]  Ming-Qun Xu,et al.  Protein Splicing of the Saccharomyces cerevisiae VMA Intein without the Endonuclease Motifs* , 1997, The Journal of Biological Chemistry.

[58]  Francine B. Perler,et al.  InBase: the Intein Database , 2002, Nucleic Acids Res..

[59]  H. Mootz,et al.  Regioselective cysteine bioconjugation by appending a labeled cystein tag to a protein by using protein splicing in trans. , 2007, Angewandte Chemie.

[60]  Christian P. R. Hackenberger,et al.  Chemoselektive Ligations‐ und Modifikationsstrategien für Peptide und Proteine , 2008 .

[61]  M. Howarth,et al.  Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase , 2005, Nature Methods.

[62]  Christopher J. Noren,et al.  Protein‐Spleißen: Mechanismus und Anwendungen , 2000 .

[63]  K V Mills,et al.  Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[64]  Jack Benner,et al.  Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. , 1998, Nucleic acids research.

[65]  H. Paulus,et al.  An in vivo screening system against protein splicing useful for the isolation of non-splicing mutants or inhibitors of the RecA intein of Mycobacterium tuberculosis. , 2002, Gene.

[66]  M. Southworth,et al.  Control of protein splicing by intein fragment reassembly , 1998, The EMBO journal.

[67]  T. Muir,et al.  Protein semi-synthesis in living cells. , 2003, Journal of the American Chemical Society.

[68]  Oliver Seitz,et al.  Native chemische Verknüpfung mit Valin , 2008 .

[69]  S. Benkovic,et al.  Structural requirements for the biosynthesis of backbone cyclic peptide libraries. , 2001, Chemistry & biology.

[70]  P. Kane,et al.  Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. , 1990, Science.

[71]  O. Schueler‐Furman,et al.  Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family , 2009, Nucleic acids research.

[72]  Peter G. Schultz,et al.  Expanding the genetic code. , 2006 .

[73]  Marjeta Urh,et al.  HaloTag: a novel protein labeling technology for cell imaging and protein analysis. , 2008, ACS chemical biology.

[74]  H. Mootz,et al.  Expanding the Scope of Protein Trans‐Splicing to Fragment Ligation of an Integral Membrane Protein: Towards Modulation of Porin‐Based Ion Channels by Chemical Modification , 2009, Chembiochem : a European journal of chemical biology.

[75]  T. Muir,et al.  Expressed protein ligation: a general method for protein engineering. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[76]  T. C. Evans,et al.  Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803. , 2001, Biochemistry.

[77]  T. Muir.  Semisynthesis of proteins by expressed protein ligation. , 2003, Annual review of biochemistry.

[78]  Uwe Linne,et al.  Ligation eines synthetischen Peptids an den N‐Terminus eines rekombinanten Proteins durch semisynthetisches trans‐Proteinspleißen , 2006 .

[79]  Gil Amitai,et al.  Distribution of split DnaE inteins in cyanobacteria , 2003, Molecular microbiology.

[80]  T. Muir,et al.  Development of a tandem protein trans-splicing system based on native and engineered split inteins. , 2005, Journal of the American Chemical Society.

[81]  H. Iwai,et al.  Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans‐Splicing , 2008, Chembiochem : a European journal of chemical biology.

[82]  Yoshio Umezawa,et al.  A genetic approach to identifying mitochondrial proteins , 2003, Nature Biotechnology.

[83]  Tom W Muir,et al.  Post-translational enzyme activation in an animal via optimized conditional protein splicing. , 2007, Nature chemical biology.

[84]  S. Züger,et al.  Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies , 2005, Nature Biotechnology.

[85]  D. Söll,et al.  Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans. , 2006, Journal of molecular biology.

[86]  Georgios Skretas,et al.  Regulation of protein activity with small‐molecule‐controlled inteins , 2005, Protein science : a publication of the Protein Society.

[87]  Neil L Kelleher,et al.  Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[88]  Shmuel Pietrokovski,et al.  Protein Splicing and Auto-cleavage of Bacterial Intein-like Domains Lacking a C′-flanking Nucleophilic Residue* , 2004, Journal of Biological Chemistry.

[89]  T. Muir,et al.  Protein splicing triggered by a small molecule. , 2002, Journal of the American Chemical Society.

[90]  David R. Liu,et al.  Directed evolution of ligand dependence: small-molecule-activated protein splicing. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[91]  H. Mootz,et al.  Site‐Specific Chemical Modification of Proteins with a Prelabelled Cysteine Tag Using the Artificially Split Mxe GyrA Intein , 2008, Chembiochem : a European journal of chemical biology.

[92]  E. Koonin,et al.  Crystal Structure of a Hedgehog Autoprocessing Domain: Homology between Hedgehog and Self-Splicing Proteins , 1997, Cell.

[93]  Amy B Tyszkiewicz,et al.  Activation of protein splicing with light in yeast , 2008, Nature Methods.

[94]  H. Paulus,et al.  Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments. , 1998, Gene.

[95]  Li Wang,et al.  Expression of split dnaE genes and trans-splicing of DnaE intein in the developmental cyanobacterium Anabaena sp. PCC 7120. , 2006, Research in microbiology.

[96]  Joachim Zettler,et al.  The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans‐splicing reaction , 2009, FEBS letters.

[97]  Noren,et al.  Dissecting the Chemistry of Protein Splicing and Its Applications. , 2000, Angewandte Chemie.

[98]  T. C. Evans,et al.  Mechanistic and kinetic considerations of protein splicing. , 2002, Chemical reviews.

[99]  R. Raines,et al.  Chemical synthesis of proteins. , 2005, Annual review of biophysics and biomolecular structure.

[100]  Tom W Muir,et al.  Protein ligation: an enabling technology for the biophysical analysis of proteins , 2006, Nature Methods.

[101]  S. Benkovic,et al.  Production of cyclic peptides and proteins in vivo. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[102]  T. Muir,et al.  Protein splicing and its applications. , 2000, Genetic engineering.