Microsphere microscopic imaging with the coherent light

The microsphere has great potential to improve the resolution of the system. The high frequency information of the object can be collected by the microsphere to enhance the imaging resolution. However, the unavoidable chromatic dispersion exists in the microsphere incoherence imaging, which reduces the image quality. In this paper, the microsphere microscopy system is designed with the coherent light. The polystyrene (PS) microspheres with the diameter of 50μm and 90μm are applied, and their refractive index is 1.59. The object is a transmission grating with a cycle of 1.2μm and line spacing of 600 nm. The result indicates that the grating can be clearly detected, and the magnification of the microsphere with the diameter of 50μm and 90μm is 2.33 and 1.96 respectively. Comparing with the traditional white light microscopy, the imaging contrast has been improved though the speckle noise is introduced for coherent light. Therefore the microsphere has the potential to improve the resolution of the phase-contrast imaging.

[1]  Shigeo Kubota,et al.  Very efficient speckle contrast reduction realized by moving diffuser device. , 2010, Applied optics.

[2]  Jarod C Finlay,et al.  Optical super-resolution imaging by high-index microspheres embedded in elastomers. , 2015, Optics letters.

[3]  Lin Li,et al.  Rapid super-resolution imaging of sub-surface nanostructures beyond diffraction limit by high refractive index microsphere optical nanoscopy , 2015 .

[4]  J. Pendry,et al.  Negative refraction makes a perfect lens , 2000, Physical review letters.

[5]  Allen Taflove,et al.  Optical analysis of nanoparticles via enhanced backscattering facilitated by 3-D photonic nanojets. , 2005, Optics express.

[6]  B Javidi,et al.  Random resampling masks: a non-Bayesian one-shot strategy for noise reduction in digital holography. , 2013, Optics letters.

[7]  Stephen R Quake,et al.  Colloidal lenses allow high-temperature single-molecule imaging and improve fluorophore photostability. , 2010, Nature nanotechnology.

[8]  N. Fang,et al.  Sub–Diffraction-Limited Optical Imaging with a Silver Superlens , 2005, Science.

[9]  E. Wolf,et al.  Principles of Optics (7th Ed) , 1999 .

[10]  O. Haeberlé,et al.  High-resolution three-dimensional tomographic diffractive microscopy of transparent inorganic and biological samples. , 2009, Optics letters.

[11]  Zengbo Wang,et al.  Optical virtual imaging at 50 nm lateral resolution with a white-light nanoscope. , 2011, Nature communications.

[12]  Tao Wang,et al.  Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy , 2013, Light: Science & Applications.

[13]  Zhaowei Liu,et al.  Superlenses to overcome the diffraction limit. , 2008, Nature materials.

[14]  Xu Liu,et al.  Microsphere based microscope with optical super-resolution capability , 2011 .

[15]  Philip Kim,et al.  Near-field focusing and magnification through self-assembled nanoscale spherical lenses , 2009, Nature.

[16]  Zhaowei Liu,et al.  Far-Field Optical Hyperlens Magnifying Sub-Diffraction-Limited Objects , 2007, Science.

[17]  Yang Wang,et al.  Near-field focusing of the dielectric microsphere with wavelength scale radius. , 2013, Optics express.

[18]  Zengbo Wang,et al.  Overcoming the diffraction limit induced by microsphere optical nanoscopy , 2013 .