Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice

Increasing crop yield is a major challenge for modern agriculture. The development of new plant types, which is known as ideal plant architecture (IPA), has been proposed as a means to enhance rice yield potential over that of existing high-yield varieties. Here, we report the cloning and characterization of a semidominant quantitative trait locus, IPA1 (Ideal Plant Architecture 1), which profoundly changes rice plant architecture and substantially enhances rice grain yield. The IPA1 quantitative trait locus encodes OsSPL14 (SOUAMOSA PROMOTER BINDING PROTEIN-LIKE 14) and is regulated by microRNA (miRNA) OsmiR156 in vivo. We demonstrate that a point mutation in OsSPL14 perturbs OsmiR156-directed regulation of OsSPL14, generating an 'ideal' rice plant with a reduced tiller number, increased lodging resistance and enhanced grain yield. Our study suggests that OsSPL14 may help improve rice grain yield by facilitating the breeding of new elite rice varieties.

[1]  D. Bartel,et al.  MicroRNAS and their regulatory roles in plants. , 2006, Annual review of plant biology.

[2]  Detlef Weigel,et al.  The Sequential Action of miR156 and miR172 Regulates Developmental Timing in Arabidopsis , 2009, Cell.

[3]  Q. Qian,et al.  LAZY1 controls rice shoot gravitropism through regulating polar auxin transport , 2007, Cell Research.

[4]  Gang Wu,et al.  Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3 , 2006, Development.

[5]  Xinwen Liang,et al.  Arabidopsis AtSPL14, a plant-specific SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1. , 2005, The Plant journal : for cell and molecular biology.

[6]  G. Khush.  Breaking the yield frontier of rice , 1995 .

[7]  K. Chong,et al.  A practical vector for efficient knockdown of gene expression in rice (Oryza sativa L.) , 2004, Plant Molecular Biology Reporter.

[8]  Graham J King,et al.  A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening , 2006, Nature Genetics.

[9]  M. Todesco,et al.  Target mimicry provides a new mechanism for regulation of microRNA activity , 2007, Nature Genetics.

[10]  Heinz Saedler,et al.  The miRNA156/157 recognition element in the 3' UTR of the Arabidopsis SBP box gene SPL3 prevents early flowering by translational inhibition in seedlings. , 2007, The Plant journal : for cell and molecular biology.

[11]  P. Huijser,et al.  SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis , 2007, Plant Molecular Biology.

[12]  F. Slack,et al.  Small non-coding RNAs in animal development , 2008, Nature Reviews Molecular Cell Biology.

[13]  L. Sieburth,et al.  Widespread Translational Inhibition by Plant miRNAs and siRNAs , 2008, Science.

[14]  L. Xiong,et al.  Genomic Organization, Differential Expression, and Interaction of SQUAMOSA Promoter-Binding-Like Transcription Factors and microRNA156 in Rice1[W] , 2006, Plant Physiology.

[15]  P. Huijser,et al.  SPL8, an SBP-Box Gene That Affects Pollen Sac Development in Arabidopsis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010678. , 2003, The Plant Cell Online.

[16]  Heinz Saedler,et al.  The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in Arabidopsis , 2008, Plant Molecular Biology.

[17]  Detlef Weigel,et al.  Dual Effects of miR156-Targeted SPL Genes and CYP78A5/KLUH on Plastochron Length and Organ Size in Arabidopsis thaliana[W][OA] , 2008, The Plant Cell Online.

[18]  C. Llave,et al.  Cleavage of Scarecrow-like mRNA Targets Directed by a Class of Arabidopsis miRNA , 2002, Science.

[19]  P. Huijser,et al.  Molecular characterisation of the Arabidopsis SBP-box genes. , 1999, Gene.

[20]  H. Vaucheret,et al.  Functions of microRNAs and related small RNAs in plants , 2006, Nature Genetics.

[21]  Detlef Weigel,et al.  miR156-Regulated SPL Transcription Factors Define an Endogenous Flowering Pathway in Arabidopsis thaliana , 2009, Cell.

[22]  G. An,et al.  Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule, and laminar joint , 2007, Plant Molecular Biology.

[23]  Y. Ukai,et al.  Quantitative trait loci for phyllochron and tillering in rice , 2004, Theoretical and Applied Genetics.

[24]  P. Huijser,et al.  A new family of DNA binding proteins includes putative transcriptional regulators of the , 1996 .