Characterization of a murine model for the rapid assessment of acute photodynamic response in tumour and muscle

A murine implanted colorectal tumour model is described in which a measurement of tumour depth of necrosis is combined with a simultaneous measurement of muscle damage. The response of this model to a range of photodynamic therapy parameters was characterized using the chlorin photosensitizer mTHPC (temoporfin, Foscan®R). Both tumour depth of necrosis (measured directly and on histology) and muscle swelling correlate with the dose of photosensitizer and light, and are shown to be repeatable and consistent with published values obtained under similar conditions using established models.

[1]  M C Berenbaum,et al.  Hydroporphyrins of the meso-tetra(hydroxyphenyl)porphyrin series as tumour photosensitizers. , 1989, The Biochemical journal.

[2]  N. Williams,et al.  Adjuvant intraoperative photodynamic therapy in experimental colorectal cancer , 1995, The British journal of surgery.

[3]  H. Oppelaar,et al.  Photosensitizing efficacy of MTHPC‐PDT compared to Photofrin‐PDT in the RIF1 mouse tumour and normal skin , 2006, International journal of cancer.

[4]  R. Bonnett,et al.  The effect of photodynamic therapy on normal skeletal muscle in an animal model , 1992, Lasers in Medical Science.

[5]  J M Bland,et al.  Statistical methods for assessing agreement between two methods of clinical measurement , 1986 .

[6]  U Althaus,et al.  Effect of drug‐light interval on photodynamic therapy with meta‐tetrahydroxyphenylchlorin in malignant mesothelioma , 1993, International journal of cancer.

[7]  A. Beckett,et al.  AKUFO AND IBARAPA. , 1965, Lancet.

[8]  R. Bonnett,et al.  Selectivity ofmeso-tetra(hydroxyphenyl)porphyrins and chlorins and of photofrin II in causing photodamage in tumour, skin, muscle and bladder. The comcept of cost-benefit in analysing the results , 1993, Lasers in Medical Science.

[9]  H. Altermatt,et al.  Photodynamic therapy with m‐tetrahydroxyphenylchlorin in vivo: Optimization of the therapeutic index , 1993, International journal of cancer.

[10]  M C Berenbaum,et al.  In vivo biological activity of the components of haematoporphyrin derivative. , 1982, British Journal of Cancer.

[11]  A. Michael-Titus,et al.  Distribution and Excretion of Radiolabeled Temoporfin in a Murine Tumor Model , 1996, Photochemistry and photobiology.