Promotion of peripheral nerve growth by collagen scaffolds loaded with collagen-targeting human nerve growth factor-beta.

Nerve growth factor (NGF) plays a critical role in neuronal development and regeneration. However, the lack of efficient NGF delivery system limits its clinical application. We reported that a peptide deduced from collagenase, TKKTLRT, fused with NGF-beta could develop a collagen based NGF targeting delivery system. Our results showed that this peptide could allow fused NGF-beta bind to collagen specifically. In addition, we found that the polypeptide could result in a 2.3-fold increase in the expression level and a significant improvement of bioactivity of fused NGF-beta. In the in vivo function study, collagen membranes loaded with the collagen binding NGF enhanced the nerve growth. Thus, the targeting wound repair system could be important for the repair of peripheral nerve injury.

[1]  R. Levi‐montalcini.  The nerve growth factor: thirty‐five years later. , 1987, The EMBO journal.

[2]  D. Olton,et al.  Human nerve growth factor improves spatial memory in aged but not in young rats , 1994, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[3]  Jeffrey C. Petruska,et al.  The many functions of nerve growth factor: multiple actions on nociceptors , 2004, Neuroscience Letters.

[4]  R. Corvò,et al.  Retinoic acid modulates the radiosensitivity of head-and-neck squamous carcinoma cells grown in collagen gel. , 2002, International journal of radiation oncology, biology, physics.

[5]  E. Transfeldt,et al.  The effect of the platelet derived wound healing formula and the nerve growth factor on the experimentally injured spinal cord , 1996, Spinal Cord.

[6]  Hanry Yu,et al.  Peripheral nerve regeneration with sustained release of poly(phosphoester) microencapsulated nerve growth factor within nerve guide conduits. , 2003, Biomaterials.

[7]  P. Brachet,et al.  Intracerebral Implantation of NGF-Releasing Biodegradable Microspheres Protects Striatum against Excitotoxic Damage , 2000, Experimental Neurology.

[8]  J. Andrades,et al.  Engineering, Expression, and Renaturation of a Collagen-Targeted Human bFGF Fusion Protein , 2001, Growth factors.

[9]  G. Terenghi,et al.  Peripheral nerve regeneration and neurotrophic factors , 1999, Journal of anatomy.

[10]  D. Chau,et al.  The cellular response to transglutaminase-cross-linked collagen. , 2005, Biomaterials.

[11]  J. G. Marblestone,et al.  Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO , 2006, Protein science : a publication of the Protein Society.

[12]  R. Brentani,et al.  Collagen binding site in collagenase can be determined using the concept of sense-antisense peptide interactions. , 1992, The Journal of biological chemistry.

[13]  J. Benoit,et al.  Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons. , 2000, Biomaterials.

[14]  O. Matsushita,et al.  A Study of the Collagen-binding Domain of a 116-kDaClostridium histolyticum Collagenase* , 1998, The Journal of Biological Chemistry.

[15]  L. Maffei,et al.  Intranasal administration of nerve growth factor (NGF) rescues recognition memory deficits in AD11 anti-NGF transgenic mice. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[16]  M. Gibson,et al.  Microfibril-associated Glycoprotein-1 (MAGP-1) Binds to the Pepsin-resistant Domain of the α3(VI) Chain of Type VI Collagen* , 1997, The Journal of Biological Chemistry.

[17]  B. Winblad,et al.  Intracerebroventricular Infusion of Nerve Growth Factor in Three Patients with Alzheimer’s Disease , 1998, Dementia and Geriatric Cognitive Disorders.

[18]  Hang Lin,et al.  The effect of collagen-targeting platelet-derived growth factor on cellularization and vascularization of collagen scaffolds. , 2006, Biomaterials.

[19]  A. Shih,et al.  Mutagenesis identifies amino-terminal residues of nerve growth factor necessary for Trk receptor binding and biological activity. , 1994, The Journal of biological chemistry.

[20]  M E Nimni,et al.  The molecular organization of collagen and its role in determining the biophysical properties of the connective tissues. , 1980, Biorheology.

[21]  James N. Campbell,et al.  Complications from silicon‐polymer intubulation of nerves , 1989, Microsurgery.

[22]  W. Friess,et al.  Collagen--biomaterial for drug delivery. , 1998, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[23]  T. Tudyka,et al.  Glutathione S‐transferase can be used as a C‐terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli , 1997, Protein science : a publication of the Protein Society.

[24]  E. Mufson,et al.  Nerve growth factor: structure, function and therapeutic implications for Alzheimer's disease. , 2003, Current drug targets. CNS and neurological disorders.

[25]  Pernilla Turner,et al.  Optimized expression of soluble cyclomaltodextrinase of thermophilic origin in Escherichia coli by using a soluble fusion-tag and by tuning of inducer concentration. , 2005, Protein expression and purification.

[26]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[27]  D. Waugh,et al.  Single amino acid substitutions on the surface of Escherichia coli maltose‐binding protein can have a profound impact on the solubility of fusion proteins , 2001, Protein science : a publication of the Protein Society.

[28]  C. M. Agrawal,et al.  Technique to control pH in vicinity of biodegrading PLA-PGA implants. , 1997, Journal of biomedical materials research.

[29]  Josef Donnerer,et al.  Regeneration of Primary Sensory Neurons , 2003, Pharmacology.

[30]  P. Keeling,et al.  Novel ubiquitin fusion proteins: ribosomal protein P1 and actin. , 2003, Journal of molecular biology.

[31]  T. Desai,et al.  Characterization of PC12 cell proliferation and differentiation-stimulated by ECM adhesion proteins and neurotrophic factors , 2003, Journal of materials science. Materials in medicine.

[32]  U. Baumann,et al.  Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm. , 2003, Journal of biochemistry.

[33]  Jui-Sheng Sun,et al.  Biocompatibility of NGF-grafted GTG membranes for peripheral nerve repair using cultured Schwann cells. , 2004, Biomaterials.

[34]  O. Liew,et al.  Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. , 2005, Protein expression and purification.