Electromagnetically Stimulated SAOS-2 Osteoblasts inside a Porous Hydroxyapatite Scaffold in Vitro

Several studies suggest that the modification of a biomaterial surfaceplay an important role in bone tissue engineering. In this study we have followeda biomimetic strategy where electromagnetically stimulated SAOS-2 osteoblasts,from a human osteosarcoma cell line, proliferated and built their extracellular matrixinside a porous hydroxyapatite scaffold. In comparison with control staticconditions, the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz)increased the cell proliferation and the production of bone proteins (decorin, osteocalcin,osteopontin, type-I collagen, and type-III collagen), with a consequentsurface coating of the scaffold. The physical stimulus was aimed at obtaining abiomimetic modification of the internal porous surface of the hydroxyapatite scaffold.The cell-biomaterial construct could be used as an implant for bone repair inclinical applications.

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