Enhanced osteogenic activity of poly ether ether ketone using calcium plasma immersion ion implantation.

As a promising implantable material, poly ether ether ketone (PEEK) possesses similar elastic modulus to that of cortical bones yet suffers from bio-inertness and poor osteogenic properties, which limits its application as orthopedic implants. In this work, calcium is introduced onto PEEK surface using calcium plasma immersion ion implantation (Ca-PIII). The results obtained from scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) confirm the modified layer with varying contents of calcium are formed on PEEK surfaces. Water contact angle measurements reveal the increasing hydrophobicity of both Ca-PIII treated surfaces. In vitro cell adhesion, viability assay, alkaline phosphatase activity and collagen secretion analyses disclose improved the adhesion, proliferation, and osteo-differentiation of rat bone mesenchymal stem cells (bMSCs) on Ca-PIII treated surfaces. The obtained results indicate that PEEK surface with enhanced osteogenic activity can be produced by calcium incorporation.

[1]  T. Albrektsson,et al.  Which surface properties enhance bone response to implants? Comparison of oxidized magnesium, TiUnite, and Osseotite implant surfaces. , 2006, The International journal of prosthodontics.

[2]  P. Chu,et al.  Biological actions of silver nanoparticles embedded in titanium controlled by micro-galvanic effects. , 2011, Biomaterials.

[3]  R. Shelton,et al.  Influence of calcium phosphate crystal assemblies on the proliferation and osteogenic gene expression of rat bone marrow stromal cells. , 2007, Biomaterials.

[4]  M. Lewandowska-Szumieł,et al.  Effect of dual ion implantation of calcium and phosphorus on the properties of titanium. , 2005, Biomaterials.

[5]  S. Kurtz,et al.  PEEK biomaterials in trauma, orthopedic, and spinal implants. , 2007, Biomaterials.

[6]  Ming Xu,et al.  Mechanical and biological characteristics of diamond-like carbon coated poly aryl-ether-ether-ketone. , 2010, Biomaterials.

[7]  P. Chu,et al.  Multilevel surface engineering of nanostructured TiO2 on carbon-fiber-reinforced polyetheretherketone. , 2014, Biomaterials.

[8]  Xianlong Zhang,et al.  Synergistic effects of dual Zn/Ag ion implantation in osteogenic activity and antibacterial ability of titanium. , 2014, Biomaterials.

[9]  M Navarro,et al.  Biomaterials in orthopaedics , 2008, Journal of The Royal Society Interface.

[10]  W. Wilkison,et al.  Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. , 2001, Tissue engineering.

[11]  E. Brown,et al.  Extracellular calcium sensing and extracellular calcium signaling. , 2001, Physiological reviews.

[12]  P. Chu,et al.  Structure and properties of Ca-plasma-implanted titanium , 2005 .

[13]  P. Chu,et al.  Hybrid processes based on plasma immersion ion implantation: a brief review , 2004 .

[14]  J. Westendorf,et al.  Polyetheretherketone--cytotoxicity and mutagenicity in vitro. , 2002, Biomaterials.

[15]  Y. Sul,et al.  Biomechanical measurements of calcium-incorporated oxidized implants in rabbit bone: effect of calcium surface chemistry of a novel implant. , 2004, Clinical implant dentistry and related research.

[16]  W. C. Liu,et al.  Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites. , 2009, Biomaterials.

[17]  Paul K. Chu,et al.  Surface nano-functionalization of biomaterials , 2010 .

[18]  P J Prendergast,et al.  Stress-concentrating effect of resorption lacunae in trabecular bone. , 2006, Journal of biomechanics.

[19]  Helmut Münstedt,et al.  Effect of βTCP filled polyetheretherketone on osteoblast cell proliferation in vitro , 2006 .

[20]  A. Turner,et al.  Polyetheretherketone as a biomaterial for spinal applications. , 2006, Biomaterials.

[21]  G. Gronowicz,et al.  The in vitro response of human osteoblasts to polyetheretherketone (PEEK) substrates compared to commercially pure titanium. , 2008, Biomaterials.

[22]  Paul K. Chu,et al.  Recent developments and applications of plasma immersion ion implantation , 2004 .

[23]  P. Chu.  Plasma surface treatment of artificial orthopedic and cardiovascular biomaterials , 2007 .

[24]  P. Chu,et al.  Enhanced osteointegration on tantalum-implanted polyetheretherketone surface with bone-like elastic modulus. , 2015, Biomaterials.

[25]  P Ducheyne,et al.  Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. , 1999, Biomaterials.

[26]  B D Boyan,et al.  Role of material surfaces in regulating bone and cartilage cell response. , 1996, Biomaterials.

[27]  Fanhao Meng,et al.  Osteogenic activity and antibacterial effect of zinc ion implanted titanium. , 2014, Colloids and surfaces. B, Biointerfaces.

[28]  K. Liao,et al.  Tensile properties, tension-tension fatigue and biological response of polyetheretherketone-hydroxyapatite composites for load-bearing orthopedic implants. , 2003, Biomaterials.

[29]  K. Khor,et al.  Mechanical properties of injection molded hydroxyapatite-polyetheretherketone biocomposites , 2003 .

[30]  Yumei Zhang,et al.  Osteoblast behavior on polytetrafluoroethylene modified by long pulse, high frequency oxygen plasma immersion ion implantation. , 2010, Biomaterials.

[31]  P. Chu,et al.  Applications of plasma immersion ion implantation in microelectronics — a brief review , 2001 .