论文信息 - Automated determination of selected water-soluble vitamins in tablets using a bench-top robotic system coupled to reversed-phase (RP-18) HPLC with UV detection.

Automated determination of selected water-soluble vitamins in tablets using a bench-top robotic system coupled to reversed-phase (RP-18) HPLC with UV detection.

The determination of folic acid, nicotinamide, nicotinic acid, riboflavin, riboflavin-5'-phosphate, pyridoxine and thiamine in tablets has been successfully automated using a bench-top robotic system (Zymark Tablet Processing Workstation II) coupled to reversed-phase (RP-18) HPLC with UV-detection. The new automated methods have been validated and were found to be applicable in routine analysis for all common types of multivitamin tablets. The precision is exemplified by relative standard deviations of 5.4% for folic acid, 2.1% for nicotinamide, 1.6% for pyridoxine, 0.7% for riboflavin and 1.1% for thiamine using film-coated tablets as sample. Mean recoveries obtained during spiking experiments were in the range of 95.2-103.9%.

[1] G. I. Baram,et al. Determination of Water- and Fat-Soluble Vitamins in Multivitamin Preparations by High-Performance Liquid Chromatography , 2002 .

[2] S M Han,et al. Transfer from manual to automated sample preparation: a case study. , 1999, Journal of pharmaceutical and biomedical analysis.

[3] W. Landen,et al. Vitamin Analysis for the Health and Food Sciences , 1998 .

[4] Feng Chen,et al. Simultaneous determination of nine water‐soluble vitamins in pharmaceutical preparations by high‐performance liquid chromatography with diode array detection , 2001 .

[5] M. D. Luque de Castro,et al. Fully automated determination of vitamins A and E in milk-based products using a robotic station-HPLC arrangement. , 1999, The Analyst.

[6] K. Reilly,et al. Automated sample preparation of Roxifiban tablets: transfer of a manual method to an automated workstation. , 2000, Journal of pharmaceutical and biomedical analysis.