Three-dimensional continuous particle focusing in a microfluidic channel via standing surface acoustic waves (SSAW).

Three-dimensional (3D) continuous microparticle focusing has been achieved in a single-layer polydimethylsiloxane (PDMS) microfluidic channel using a standing surface acoustic wave (SSAW). The SSAW was generated by the interference of two identical surface acoustic waves (SAWs) created by two parallel interdigital transducers (IDTs) on a piezoelectric substrate with a microchannel precisely bonded between them. To understand the working principle of the SSAW-based 3D focusing and investigate the position of the focal point, we computed longitudinal waves, generated by the SAWs and radiated into the fluid media from opposite sides of the microchannel, and the resultant pressure and velocity fields due to the interference and reflection of the longitudinal waves. Simulation results predict the existence of a focusing point which is in good agreement with our experimental observations. Compared with other 3D focusing techniques, this method is non-invasive, robust, energy-efficient, easy to implement, and applicable to nearly all types of microparticles.

[1]  Hywel Morgan,et al.  High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection. , 2006, Biosensors & bioelectronics.

[2]  Joshua B. Edel,et al.  Hydrodynamic focusing in microstructures: Improved detection efficiencies in subfemtoliter probe volumes , 2007 .

[3]  Chih-Ming Ho,et al.  Electrokinetic bioprocessor for concentrating cells and molecules. , 2004, Analytical chemistry.

[4]  George M. Whitesides,et al.  Extending Microcontact Printing as a Microlithographic Technique , 1997 .

[5]  S M Gruner,et al.  Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Alex Groisman,et al.  High-throughput and high-resolution flow cytometry in molded microfluidic devices. , 2006, Analytical chemistry.

[7]  Thomas Laurell,et al.  Acoustic control of suspended particles in micro fluidic chips. , 2004, Lab on a chip.

[8]  Daniel Ahmed,et al.  A millisecond micromixer via single-bubble-based acoustic streaming. , 2009, Lab on a chip.

[9]  James Friend,et al.  Rapid microscale in-gel processing and digestion of proteins using surface acoustic waves. , 2010, Lab on a chip.

[10]  Xuezhu Liu,et al.  Electrokinetically based approach for single-nucleotide polymorphism discrimination using a microfluidic device. , 2005, Analytical chemistry.

[11]  T. Laurell,et al.  Continuous separation of lipid particles from erythrocytes by means of laminar flow and acoustic standing wave forces. , 2005, Lab on a chip.

[12]  Liesbet Lagae,et al.  Manipulation of magnetic particles on chip by magnetophoretic actuation and dielectrophoretic levitation , 2007 .

[13]  Despina Bazou,et al.  Molecular adhesion development in a neural cell monolayer forming in an ultrasound trap , 2005, Molecular membrane biology.

[14]  Stephen R Quake,et al.  Velocity‐independent microfluidic flow cytometry , 2002, Electrophoresis.

[15]  Steven W Graves,et al.  Ultrasonic particle‐concentration for sheathless focusing of particles for analysis in a flow cytometer , 2006, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[16]  Thomas Laurell,et al.  Chip integrated strategies for acoustic separation and manipulation of cells and particles. , 2007, Chemical Society reviews.

[17]  C. Grigoropoulos,et al.  Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses. , 2009, Lab on a chip.

[18]  Ruey-Jen Yang,et al.  Three-dimensional hydrodynamic focusing in two-layer polydimethylsiloxane (PDMS) microchannels , 2007 .

[19]  Z Guttenberg,et al.  Flow profiling of a surface-acoustic-wave nanopump. , 2004, Physical review. E, Statistical, nonlinear, and soft matter physics.

[20]  Liesbet Lagae,et al.  Cell manipulation with magnetic particles toward microfluidic cytometry , 2009 .

[21]  T. Huang,et al.  Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. , 2009, Lab on a chip.

[22]  Bruce D. Bowen,et al.  Measurement of ultrasonic forces for particle–liquid separations , 1997 .

[23]  T. Huang,et al.  Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[24]  Tsung-Tsong Wu,et al.  Gradient-index phononic crystals , 2009 .

[25]  J. Michael Ramsey,et al.  Electrokinetic Focusing in Microfabricated Channel Structures , 1997 .

[26]  N. Sundararajan,et al.  Three-dimensional hydrodynamic focusing in polydimethylsiloxane (PDMS) microchannels , 2004, Journal of Microelectromechanical Systems.

[27]  D. Di Carlo,et al.  Sheathless inertial cell ordering for extreme throughput flow cytometry. , 2010, Lab on a chip.

[28]  Jinjie Shi,et al.  Wide-band acoustic collimating by phononic crystal composites , 2008 .

[29]  Thomas Laurell,et al.  Trapping of microparticles in the near field of an ultrasonic transducer. , 2005, Ultrasonics.

[30]  C.-H. Chou,et al.  Lens design for acoustic microscopy , 1988, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[31]  Alex Groisman,et al.  Two-dimensional hydrodynamic focusing in a simple microfluidic device , 2005 .

[32]  Daniel Ahmed,et al.  Focusing microparticles in a microfluidic channel with standing surface acoustic waves (SSAW). , 2008, Lab on a chip.

[33]  Elinore M Mercer,et al.  Microfluidic sorting of mammalian cells by optical force switching , 2005, Nature Biotechnology.

[34]  Shin-ichiro Umemura,et al.  Studies on particle separation by acoustic radiation force and electrostatic force , 1996 .

[35]  D A Weitz,et al.  Surface acoustic wave actuated cell sorting (SAWACS). , 2010, Lab on a chip.

[36]  Leslie Y Yeo,et al.  Exploitation of surface acoustic waves to drive size-dependent microparticle concentration within a droplet. , 2010, Lab on a chip.

[37]  David Erickson,et al.  Electrokinetic microfluidic devices for rapid, low power drug delivery in autonomous microsystems. , 2008, Lab on a chip.

[38]  Daniel Ahmed,et al.  A fast microfluidic mixer based on acoustically driven sidewall-trapped microbubbles , 2009 .

[39]  Alex Groisman,et al.  Ultrafast microfluidic mixer with three-dimensional flow focusing for studies of biochemical kinetics. , 2010, Lab on a chip.

[40]  T. Laurell,et al.  Free flow acoustophoresis: microfluidic-based mode of particle and cell separation. , 2007, Analytical chemistry.

[41]  P. Wong,et al.  Electrokinetics in micro devices for biotechnology applications , 2004, IEEE/ASME Transactions on Mechatronics.

[42]  J.A. Schwartz,et al.  A three-dimensional dielectrophoretic particle focusing channel for microcytometry applications , 2005, Journal of Microelectromechanical Systems.

[43]  Mehmet Toner,et al.  Particle focusing in staged inertial microfluidic devices for flow cytometry. , 2010, Analytical chemistry.

[44]  Tony Jun Huang,et al.  Acoustic mirage in two-dimensional gradient-index phononic crystals , 2009 .

[45]  Tony Jun Huang,et al.  "Microfluidic drifting"--implementing three-dimensional hydrodynamic focusing with a single-layer planar microfluidic device. , 2007, Lab on a chip.

[46]  Conrad D. James,et al.  High-efficiency magnetic particle focusing using dielectrophoresis and magnetophoresis in a microfluidic device , 2010 .

[47]  R. Austin,et al.  Hydrodynamic Focusing on a Silicon Chip: Mixing Nanoliters in Microseconds , 1998 .

[48]  Steven W Graves,et al.  Analytical performance of an ultrasonic particle focusing flow cytometer. , 2007, Analytical chemistry.

[49]  Chunyang Zhang,et al.  Comparative quantification of nucleic acids using single-molecule detection and molecular beacons. , 2005, The Analyst.

[50]  S. Quake,et al.  A microfabricated fluorescence-activated cell sorter , 1999, Nature Biotechnology.

[51]  Larisa A. Kuznetsova,et al.  Microparticle concentration in short path length ultrasonic resonators: Roles of radiation pressure and acoustic streaming , 2004 .

[52]  Daniel Ahmed,et al.  Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW). , 2009, Lab on a chip.

[53]  Chih-Ming Ho,et al.  Single-molecule tracing on a fluidic microchip for quantitative detection of low-abundance nucleic acids. , 2005, Journal of the American Chemical Society.

[54]  J. Chang,et al.  Simultaneous counting of two subsets of leukocytes using fluorescent silica nanoparticles in a sheathless microchip flow cytometer. , 2010, Lab on a chip.

[55]  J. Michael Ramsey,et al.  Microchip flow cytometry using electrokinetic focusing. , 1999, Analytical chemistry.

[56]  S. Hagen,et al.  Laminar-flow fluid mixer for fast fluorescence kinetics studies. , 2002, Biophysical journal.

[57]  Shizhi Qian,et al.  Three-dimensional electrokinetic particle focusing in a rectangular microchannel. , 2010, Journal of colloid and interface science.

[58]  Xuezhu Liu,et al.  Electrokinetically controlled DNA hybridization microfluidic chip enabling rapid target analysis. , 2004, Analytical chemistry.

[59]  Young-Ho Cho,et al.  A two-dimensional particle focusing channel using the positive dielectrophoresis (PDEP) guided by a dielectric structure between two planar electrodes , 2008, 2008 IEEE 21st International Conference on Micro Electro Mechanical Systems.

[60]  Daniel L. Feeback,et al.  Microfabrication and test of a three-dimensional polymer hydro-focusing unit for flow cytometry applications , 2005 .

[61]  R. Tompkins,et al.  Continuous inertial focusing, ordering, and separation of particles in microchannels , 2007, Proceedings of the National Academy of Sciences.

[62]  Alex Terray,et al.  "Off-the-shelf" 3-D microfluidic nozzle. , 2010, Lab on a chip.